Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus

3 pags, 2 figs, 1 tab

Detalhes bibliográficos
Autores: Pérez-Dorado, Inmaculada, Bortolotti, Ana, Cortez, Néstor, Hermoso, Juan A.
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2008
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/249367
Acesso em linha:http://hdl.handle.net/10261/249367
Access Level:Acceso aberto
Palavra-chave:Flavodoxins
Nitrogen fixation
Rhodobacter capsulatus
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spelling Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatusPérez-Dorado, InmaculadaBortolotti, AnaCortez, NéstorHermoso, Juan A.FlavodoxinsNitrogen fixationRhodobacter capsulatus3 pags, 2 figs, 1 tabFlavodoxins are small electron-transfer proteins that contain one molecule of noncovalently bound flavin mononucleotide (FMN). The flavodoxin NifF from the photosynthetic bacterium Rhodobacter capsulatus is reduced by one electron from ferredoxin/flavodoxin:NADP(H) reductase and was postulated to be an electron donor to nitrogenase in vivo. NifF was cloned and overexpressed in Escherichia coli, purified and concentrated for crystallization using the hanging-drop vapour-diffusion method at 291 K. Crystals grew from a mixture of PEG 3350 and PEG 400 at pH 5.5 and belong to the tetragonal space group P412 12, with unit-cell parameters a = b = 66.49, c = 121.32 Å. X-ray data sets have been collected to 2.17 Å resolution. © International Union of Crystallography 2008.IP-D is a fellow of the Consejo Superior de Investigaciones Científicas (Spain). AB is a fellow of CONICET (Argentina) and NC is a staff investigator of the same institution. This work was supported by grants BFU2005-01645, BIO2003-01952 and BFU2006-10288 from Dirección General de Investigación, by 08.2/0030.1/2003 from the Comunidad de Madrid and by grant PIP 5124 from CONICET (Argentina). This is a product of the Project ‘Factoría Española de Cristalización’ Ingenio/Consolider 2010International Union of CrystallographyConsejo Nacional de Investigaciones Científicas y Técnicas (Argentina)Comunidad de MadridFactoría Española de CristalizaciónConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120082021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/249367reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Italianohttp://dx.doi.org/10.1107/S1744309108008038Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2493672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
title Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
spellingShingle Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
Pérez-Dorado, Inmaculada
Flavodoxins
Nitrogen fixation
Rhodobacter capsulatus
title_short Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
title_full Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
title_fullStr Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
title_full_unstemmed Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
title_sort Crystallization of a flavodoxin involved in nitrogen fixation in Rhodobacter capsulatus
dc.creator.none.fl_str_mv Pérez-Dorado, Inmaculada
Bortolotti, Ana
Cortez, Néstor
Hermoso, Juan A.
author Pérez-Dorado, Inmaculada
author_facet Pérez-Dorado, Inmaculada
Bortolotti, Ana
Cortez, Néstor
Hermoso, Juan A.
author_role author
author2 Bortolotti, Ana
Cortez, Néstor
Hermoso, Juan A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
Comunidad de Madrid
Factoría Española de Cristalización
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Flavodoxins
Nitrogen fixation
Rhodobacter capsulatus
topic Flavodoxins
Nitrogen fixation
Rhodobacter capsulatus
description 3 pags, 2 figs, 1 tab
publishDate 2008
dc.date.none.fl_str_mv 2008
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/249367
url http://hdl.handle.net/10261/249367
dc.language.none.fl_str_mv Italiano
language_invalid_str_mv Italiano
dc.relation.none.fl_str_mv http://dx.doi.org/10.1107/S1744309108008038
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv International Union of Crystallography
publisher.none.fl_str_mv International Union of Crystallography
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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score 15,812429

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