A midzone-based ruler adjusts chromosome compaction to analphase spindle length

Partitioning of chromatids during mitosis requires that chromosome compaction and spindle length scale appropriately with each other. However, it is not clear whether chromosome condensation and spindle elongation are linked. Here we have used chromosome fusions to examine the impact of increased ch...

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Detalles Bibliográficos
Autor: Neurohr, Gabriel Erich
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/81932
Acceso en línea:http://hdl.handle.net/10803/81932
Access Level:acceso abierto
Palabra clave:Saccharomyces
Mitosis
Anaphase
Mitotic
Chromosome condensation
Chromosome segregation
Aurora
Ipl1
576
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spelling A midzone-based ruler adjusts chromosome compaction to analphase spindle lengthNeurohr, Gabriel ErichSaccharomycesMitosisAnaphaseMitoticChromosome condensationChromosome segregationAuroraIpl1576Partitioning of chromatids during mitosis requires that chromosome compaction and spindle length scale appropriately with each other. However, it is not clear whether chromosome condensation and spindle elongation are linked. Here we have used chromosome fusions to examine the impact of increased chromosome length during yeast mitosis. We find that yeast cells could cope with a >50% increase in the length of their longest chromosome arm by decreasing the physical length of the mitotic chromosome arm through 1) reducing the number of copies of the repetitive rDNA array and 2) by increasing the level of mitotic condensation. Consistently, cells carrying the fused chromosomes became more sensitive to loss of condensin- and its regulator polo kinase/Cdc5. Length-dependent stimulation of condensation took place during anaphase and depended on aurora/Ipl1 activity, its localization to the spindle midzone, and phosphorylation of histone H3 on Ser10, a known Ipl1 substrate. The anaphase spindle therefore may function as a ruler to adapt the condensation of chromosomes to spindle length. Consistent with this, chromosome condensation levels correlate with the length of anaphase spindles.Programa de doctorat en BiomedicinaUniversitat Pompeu FabraMendoza, ManuelUniversitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut201220122012info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion138 p.application/pdfapplication/pdfhttp://hdl.handle.net/10803/81932TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/819322026-06-14T12:46:07Z
dc.title.none.fl_str_mv A midzone-based ruler adjusts chromosome compaction to analphase spindle length
title A midzone-based ruler adjusts chromosome compaction to analphase spindle length
spellingShingle A midzone-based ruler adjusts chromosome compaction to analphase spindle length
Neurohr, Gabriel Erich
Saccharomyces
Mitosis
Anaphase
Mitotic
Chromosome condensation
Chromosome segregation
Aurora
Ipl1
576
title_short A midzone-based ruler adjusts chromosome compaction to analphase spindle length
title_full A midzone-based ruler adjusts chromosome compaction to analphase spindle length
title_fullStr A midzone-based ruler adjusts chromosome compaction to analphase spindle length
title_full_unstemmed A midzone-based ruler adjusts chromosome compaction to analphase spindle length
title_sort A midzone-based ruler adjusts chromosome compaction to analphase spindle length
dc.creator.none.fl_str_mv Neurohr, Gabriel Erich
author Neurohr, Gabriel Erich
author_facet Neurohr, Gabriel Erich
author_role author
dc.contributor.none.fl_str_mv Mendoza, Manuel
Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
dc.subject.none.fl_str_mv Saccharomyces
Mitosis
Anaphase
Mitotic
Chromosome condensation
Chromosome segregation
Aurora
Ipl1
576
topic Saccharomyces
Mitosis
Anaphase
Mitotic
Chromosome condensation
Chromosome segregation
Aurora
Ipl1
576
description Partitioning of chromatids during mitosis requires that chromosome compaction and spindle length scale appropriately with each other. However, it is not clear whether chromosome condensation and spindle elongation are linked. Here we have used chromosome fusions to examine the impact of increased chromosome length during yeast mitosis. We find that yeast cells could cope with a >50% increase in the length of their longest chromosome arm by decreasing the physical length of the mitotic chromosome arm through 1) reducing the number of copies of the repetitive rDNA array and 2) by increasing the level of mitotic condensation. Consistently, cells carrying the fused chromosomes became more sensitive to loss of condensin- and its regulator polo kinase/Cdc5. Length-dependent stimulation of condensation took place during anaphase and depended on aurora/Ipl1 activity, its localization to the spindle midzone, and phosphorylation of histone H3 on Ser10, a known Ipl1 substrate. The anaphase spindle therefore may function as a ruler to adapt the condensation of chromosomes to spindle length. Consistent with this, chromosome condensation levels correlate with the length of anaphase spindles.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/81932
url http://hdl.handle.net/10803/81932
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 138 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403824046735360
score 15,300724

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