Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit

Tocopherols are plant-derived isoprenoids with potent antioxidant activity, which have been implicated in the tolerance of plants to different stresses. However, tocopherol accumulation and biosynthesis in fruit, and their potential implication in postharvest chilling injury (CI), has been scarcely...

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Autores: Rey, Florencia, Rodrigo, María Jesús, Zacarías, Lorenzo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/242215
Acceso en línea:http://hdl.handle.net/10261/242215
Access Level:acceso abierto
Palabra clave:Antioxidants
Chilling injury
Gene expression
Mandarin
Tocopherols
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spelling Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruitRey, FlorenciaRodrigo, María JesúsZacarías, LorenzoAntioxidantsChilling injuryGene expressionMandarinTocopherolsTocopherols are plant-derived isoprenoids with potent antioxidant activity, which have been implicated in the tolerance of plants to different stresses. However, tocopherol accumulation and biosynthesis in fruit, and their potential implication in postharvest chilling injury (CI), has been scarcely studied. Therefore, in this work, we have investigated tocopherol accumulation and biosynthesis in the peel of mandarin fruit of three cultivars with contrasting susceptibility to CI during storage at 2 °C (‘Fortune’>‘Nova’> ‘Nadorcott’). α- and γ-tocopherol were the isoforms detected in the flavedo of the fruit, and a direct relationship between tocopherols content and CI-tolerance was found, since CI-tolerant fruit accumulated the highest tocopherol content whereas CI-sensitive fruit the lowest. Moreover, the transcriptional profiling of 14 genes related to the specific steps of tocopherol biosynthesis, and to their precursor’s synthesis, were analyzed. Upstream genes DXS1 and DXS2 (1-deoxy-d-xylulose-5-phosphate synthase) and GGDR (geranylgeranyl diphosphate reductase), involved in the supply of phytyl pyrophosphate, and the VTE3 (2-methyl-6-phytyl-1,4-benzoquinol methyltransferase) isoforms appear to be key for the differences in total tocopherol content among the cultivars at harvest. During cold storage, most genes involved in the precursors supply were up-regulated, whereas genes of the tocopherol-core pathway were in general repressed. Changes in VTE4 during cold storage may account for the differences in γ-tocopherol among cultivars. Collectively, results suggest that the concentration of tocopherols at harvest may play a function in the natural tolerance of mandarin fruit to CI, and that changes in the expression of genes during storage appear to be cold-regulated responses, rather than involved in CI tolerance.This work was funded by the research grant RTI2018-095131-B-I00 of the Ministry of Science and Innovation (Spanish Government). F.R. is the recipient of a PhD scholarship (POS_EXT_2016_1_133720) from ANII (Uruguay)Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/242215reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095131-B-I00https://doi.org/10.1016/j.postharvbio.2021.111594Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2422152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
title Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
spellingShingle Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
Rey, Florencia
Antioxidants
Chilling injury
Gene expression
Mandarin
Tocopherols
title_short Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
title_full Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
title_fullStr Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
title_full_unstemmed Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
title_sort Accumulation of tocopherols and transcriptional regulation of their biosynthesis during cold storage of mandarin fruit
dc.creator.none.fl_str_mv Rey, Florencia
Rodrigo, María Jesús
Zacarías, Lorenzo
author Rey, Florencia
author_facet Rey, Florencia
Rodrigo, María Jesús
Zacarías, Lorenzo
author_role author
author2 Rodrigo, María Jesús
Zacarías, Lorenzo
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antioxidants
Chilling injury
Gene expression
Mandarin
Tocopherols
topic Antioxidants
Chilling injury
Gene expression
Mandarin
Tocopherols
description Tocopherols are plant-derived isoprenoids with potent antioxidant activity, which have been implicated in the tolerance of plants to different stresses. However, tocopherol accumulation and biosynthesis in fruit, and their potential implication in postharvest chilling injury (CI), has been scarcely studied. Therefore, in this work, we have investigated tocopherol accumulation and biosynthesis in the peel of mandarin fruit of three cultivars with contrasting susceptibility to CI during storage at 2 °C (‘Fortune’>‘Nova’> ‘Nadorcott’). α- and γ-tocopherol were the isoforms detected in the flavedo of the fruit, and a direct relationship between tocopherols content and CI-tolerance was found, since CI-tolerant fruit accumulated the highest tocopherol content whereas CI-sensitive fruit the lowest. Moreover, the transcriptional profiling of 14 genes related to the specific steps of tocopherol biosynthesis, and to their precursor’s synthesis, were analyzed. Upstream genes DXS1 and DXS2 (1-deoxy-d-xylulose-5-phosphate synthase) and GGDR (geranylgeranyl diphosphate reductase), involved in the supply of phytyl pyrophosphate, and the VTE3 (2-methyl-6-phytyl-1,4-benzoquinol methyltransferase) isoforms appear to be key for the differences in total tocopherol content among the cultivars at harvest. During cold storage, most genes involved in the precursors supply were up-regulated, whereas genes of the tocopherol-core pathway were in general repressed. Changes in VTE4 during cold storage may account for the differences in γ-tocopherol among cultivars. Collectively, results suggest that the concentration of tocopherols at harvest may play a function in the natural tolerance of mandarin fruit to CI, and that changes in the expression of genes during storage appear to be cold-regulated responses, rather than involved in CI tolerance.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/242215
url http://hdl.handle.net/10261/242215
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095131-B-I00
https://doi.org/10.1016/j.postharvbio.2021.111594
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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