Use of targeted SNP selection for an improved anchoring of the melon (Cucumis melo L.) scaffold genome assembly

[Background]: The genome of the melon (Cucumis melo L.) double-haploid line DHL92 was recently sequenced, with 87.5 and 80.8% of the scaffold assembly anchored and oriented to the 12 linkage groups, respectively. However, insufficient marker coverage and a lack of recombination left several large, g...

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Detalles Bibliográficos
Autores: Argyris, Jason, Ruiz-Herrera, Aurora, Madriz-Masis, Pablo, Sanseverino, Walter, Morata, Jordi, Pujol, Marta, Ramos-Onsins, Sebastian E., García-Mas, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/250751
Acceso en línea:http://hdl.handle.net/10261/250751
Access Level:acceso abierto
Descripción
Sumario:[Background]: The genome of the melon (Cucumis melo L.) double-haploid line DHL92 was recently sequenced, with 87.5 and 80.8% of the scaffold assembly anchored and oriented to the 12 linkage groups, respectively. However, insufficient marker coverage and a lack of recombination left several large, gene rich scaffolds unanchored, and some anchored scaffolds unoriented. To improve the anchoring and orientation of the melon genome assembly, we used resequencing data between the parental lines of DHL92 to develop a new set of SNP markers from unanchored scaffolds.

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