Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase

Exposure of yeast to high osmolarity induces a transient activation of the Hog1 stress-activated protein kinase (SAPK), which is required for cell survival under these conditions. However, sustained activation of the SAPK results in a severe growth defect. We found that prolonged SAPK activation lea...

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Autores: Vendrell Arasa, Alexandre, Martínez Pastor, Mar, González Novo, Alberto, Pascual Ahuir, Amparo, Sinclair, David A., Proft, Markus, Posas Garriga, Francesc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/25460
Acceso en línea:http://hdl.handle.net/10230/25460
http://dx.doi.org/10.1038/embor.2011.154
Access Level:acceso abierto
Palabra clave:Saccharomyces cerevisiae -- Metabolisme
Factors de transcripció
Proteïnes
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spelling Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinaseVendrell Arasa, AlexandreMartínez Pastor, MarGonzález Novo, AlbertoPascual Ahuir, AmparoSinclair, David A.Proft, MarkusPosas Garriga, FrancescSaccharomyces cerevisiae -- MetabolismeFactors de transcripcióProteïnesExposure of yeast to high osmolarity induces a transient activation of the Hog1 stress-activated protein kinase (SAPK), which is required for cell survival under these conditions. However, sustained activation of the SAPK results in a severe growth defect. We found that prolonged SAPK activation leads to cell death, which is not observed in nma111 cells, by causing accumulation of reactive oxygen species (ROS). Mutations of the SCF(CDC4) ubiquitin ligase complex suppress cell death by preventing the degradation of Msn2 and Msn4 transcription factors. Accumulation of Msn2 and Msn4 leads to the induction of PNC1, which is an activator of the Sir2 histone acetylase. Sir2 is involved in protection against Hog1-induced cell death and can suppress Hog1-induced ROS accumulation. Therefore, cell death seems to be dictated by the balance of ROS induced by Hog1 and the protective effect of Sir2.This work was supported by grants from the Ministerio de Ciència y Innovación (BIO2009-07762) and Consolider Ingenio 2010 programme (grant CSD2007-0015), UNICELLSYS from FP7, as well as supported byFundación Marcelino Botín. F.P. is the recipient of the Institució Catalana de Recerca i Estudis Avançats Acadèmia (Generalitat de Catalunya). D.A.S. is supported by grants from the National Institute on Aging National Institutes of Health, the Ellison Medical Foundation and the Glenn Foundation for Medical Research.Nature Publishing Group201520152011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25460http://dx.doi.org/10.1038/embor.2011.154reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésEMBO reports. 2011;12(10):1062-608info:eu-repo/grantAgreement/ES/3PN/BIO2009-07762© Nature Publishing Group. http://dx.doi.org/10.1038/embor.2011.154. This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported licencehttp://creativecommons.org/licenses/by-nc-sa/3.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/254602026-05-29T05:05:01Z
dc.title.none.fl_str_mv Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
title Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
spellingShingle Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
Vendrell Arasa, Alexandre
Saccharomyces cerevisiae -- Metabolisme
Factors de transcripció
Proteïnes
title_short Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
title_full Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
title_fullStr Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
title_full_unstemmed Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
title_sort Sir2 histone deacetylase prevents programmed cell death caused by sustained activation of the Hog1 stress-activated protein kinase
dc.creator.none.fl_str_mv Vendrell Arasa, Alexandre
Martínez Pastor, Mar
González Novo, Alberto
Pascual Ahuir, Amparo
Sinclair, David A.
Proft, Markus
Posas Garriga, Francesc
author Vendrell Arasa, Alexandre
author_facet Vendrell Arasa, Alexandre
Martínez Pastor, Mar
González Novo, Alberto
Pascual Ahuir, Amparo
Sinclair, David A.
Proft, Markus
Posas Garriga, Francesc
author_role author
author2 Martínez Pastor, Mar
González Novo, Alberto
Pascual Ahuir, Amparo
Sinclair, David A.
Proft, Markus
Posas Garriga, Francesc
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Saccharomyces cerevisiae -- Metabolisme
Factors de transcripció
Proteïnes
topic Saccharomyces cerevisiae -- Metabolisme
Factors de transcripció
Proteïnes
description Exposure of yeast to high osmolarity induces a transient activation of the Hog1 stress-activated protein kinase (SAPK), which is required for cell survival under these conditions. However, sustained activation of the SAPK results in a severe growth defect. We found that prolonged SAPK activation leads to cell death, which is not observed in nma111 cells, by causing accumulation of reactive oxygen species (ROS). Mutations of the SCF(CDC4) ubiquitin ligase complex suppress cell death by preventing the degradation of Msn2 and Msn4 transcription factors. Accumulation of Msn2 and Msn4 leads to the induction of PNC1, which is an activator of the Sir2 histone acetylase. Sir2 is involved in protection against Hog1-induced cell death and can suppress Hog1-induced ROS accumulation. Therefore, cell death seems to be dictated by the balance of ROS induced by Hog1 and the protective effect of Sir2.
publishDate 2011
dc.date.none.fl_str_mv 2011
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25460
http://dx.doi.org/10.1038/embor.2011.154
url http://hdl.handle.net/10230/25460
http://dx.doi.org/10.1038/embor.2011.154
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv EMBO reports. 2011;12(10):1062-608
info:eu-repo/grantAgreement/ES/3PN/BIO2009-07762
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
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