Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv

This paper reports the glutenin diversity of a collection of 23 wild populations of the grass Brachypodium distachyon collected in the Mediterranean and southern areas of the Iberian Peninsula. The plant material studied included the three different cytotypes of this species 2n = 10, 2n = 20 and 2n...

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Detalles Bibliográficos
Autores: Hammami, R., Jouve, N., Cuadrado, Angeles, Soler, C., González, Juan M.
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/292296
Acceso en línea:http://hdl.handle.net/10261/292296
Access Level:acceso abierto
Palabra clave:Brachypodium distachyon
Glutenins
Endosperm proteins
Genetic diversity
Allopolyploidy
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spelling Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. BeauvHammami, R.Jouve, N.Cuadrado, AngelesSoler, C.González, Juan M.Brachypodium distachyonGluteninsEndosperm proteinsGenetic diversityAllopolyploidyThis paper reports the glutenin diversity of a collection of 23 wild populations of the grass Brachypodium distachyon collected in the Mediterranean and southern areas of the Iberian Peninsula. The plant material studied included the three different cytotypes of this species 2n = 10, 2n = 20 and 2n = 30. A specific method of extraction was used to isolate the glutenin subunits from the caryopsis. Separation by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed them to correspond to wheat low-molecular-weight glutenin subunits (LMW-GS). Twenty-two LMW-GS-like were identified that showed great diversity within and between populations. All the populations investigated were polymorphic for the endosperm proteins studied. The 2n = 30 forms had the largest number of subunits; these were also more diverse than those of the 2n = 10 or 2n = 20 forms. The 2n = 10 forms, the most common in the higher, interior areas of the Iberian Peninsula, showed the smallest subunit variation. In fact, negative correlations were found between subunit diversity and altitude and longitude. In contrast, a positive correlation was detected with the annual average temperature and the indices of thermicity and Mediterraneity. The similarity between the populations was estimated using the Sorensen-Dice coefficient, calculated on the basis of the presence/absence of the 22 LMW-GS proteins. The similarity indices were used to produce a dendrogram using the unweighted pair-group method with arithmetic means (UPGMA). This method produced three main groups corresponding to the three cytotypes. An analysis was made of the possible correlation between eco-geographic and climatic factors and the gene diversity and polymorphism shown in the populations. The diversity correlated positively with the number of chromosomes (2n), annual mean temperature, index of thermicity and index of Mediterraneity. In contrast, diversity correlated negatively with altitude, longitude and index of precipitation during the summer. The number of chromosomes correlated negatively with altitude, longitude and precipitation during summer and positively with all the other climatic indices. These results are coherent with the fact that diploid forms were more common in areas of the interior and at higher altitude, where the climate is more extreme. © 2011 The Author(s).Peer reviewedSpringer NatureConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/292296reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDepartamento de Medio Ambiente y AgronomíaSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2922962026-05-22T06:33:51Z
dc.title.none.fl_str_mv Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
title Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
spellingShingle Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
Hammami, R.
Brachypodium distachyon
Glutenins
Endosperm proteins
Genetic diversity
Allopolyploidy
title_short Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
title_full Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
title_fullStr Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
title_full_unstemmed Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
title_sort Prolamin storage proteins and alloploidy in wild populations of the small grass Brachypodium distachyon (L.) P. Beauv
dc.creator.none.fl_str_mv Hammami, R.
Jouve, N.
Cuadrado, Angeles
Soler, C.
González, Juan M.
author Hammami, R.
author_facet Hammami, R.
Jouve, N.
Cuadrado, Angeles
Soler, C.
González, Juan M.
author_role author
author2 Jouve, N.
Cuadrado, Angeles
Soler, C.
González, Juan M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Brachypodium distachyon
Glutenins
Endosperm proteins
Genetic diversity
Allopolyploidy
topic Brachypodium distachyon
Glutenins
Endosperm proteins
Genetic diversity
Allopolyploidy
description This paper reports the glutenin diversity of a collection of 23 wild populations of the grass Brachypodium distachyon collected in the Mediterranean and southern areas of the Iberian Peninsula. The plant material studied included the three different cytotypes of this species 2n = 10, 2n = 20 and 2n = 30. A specific method of extraction was used to isolate the glutenin subunits from the caryopsis. Separation by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed them to correspond to wheat low-molecular-weight glutenin subunits (LMW-GS). Twenty-two LMW-GS-like were identified that showed great diversity within and between populations. All the populations investigated were polymorphic for the endosperm proteins studied. The 2n = 30 forms had the largest number of subunits; these were also more diverse than those of the 2n = 10 or 2n = 20 forms. The 2n = 10 forms, the most common in the higher, interior areas of the Iberian Peninsula, showed the smallest subunit variation. In fact, negative correlations were found between subunit diversity and altitude and longitude. In contrast, a positive correlation was detected with the annual average temperature and the indices of thermicity and Mediterraneity. The similarity between the populations was estimated using the Sorensen-Dice coefficient, calculated on the basis of the presence/absence of the 22 LMW-GS proteins. The similarity indices were used to produce a dendrogram using the unweighted pair-group method with arithmetic means (UPGMA). This method produced three main groups corresponding to the three cytotypes. An analysis was made of the possible correlation between eco-geographic and climatic factors and the gene diversity and polymorphism shown in the populations. The diversity correlated positively with the number of chromosomes (2n), annual mean temperature, index of thermicity and index of Mediterraneity. In contrast, diversity correlated negatively with altitude, longitude and index of precipitation during the summer. The number of chromosomes correlated negatively with altitude, longitude and precipitation during summer and positively with all the other climatic indices. These results are coherent with the fact that diploid forms were more common in areas of the interior and at higher altitude, where the climate is more extreme. © 2011 The Author(s).
publishDate 2011
dc.date.none.fl_str_mv 2011
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/292296
url http://hdl.handle.net/10261/292296
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Departamento de Medio Ambiente y Agronomía
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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