Might exogenous circular RNAs act as protein-coding transcripts in plants?

Circular RNAs (circRNAs) are regulatory molecules involved in the modulation of gene expression. Although originally assumed as non-coding RNAs, recent studies have evidenced that animal circRNAs can act as translatable transcripts. The study of plant-circRNAs is incipient, and no autonomous coding...

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Autores: Marquez-Molins, Joan, Navarro, José A., Cervera-Seco, Luis, Pallás Benet, Vicente, Gómez, Gustavo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/262231
Acceso en línea:http://hdl.handle.net/10261/262231
Access Level:acceso abierto
Palabra clave:Circular RNAs
Plant coding circRNAs
Non canonical transcripts
Plant pathogenic RNAs
Viroids
Viroid-derived peptides
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spelling Might exogenous circular RNAs act as protein-coding transcripts in plants?Marquez-Molins, JoanNavarro, José A.Cervera-Seco, LuisPallás Benet, VicenteGómez, GustavoCircular RNAsPlant coding circRNAsNon canonical transcriptsPlant pathogenic RNAsViroidsViroid-derived peptidesCircular RNAs (circRNAs) are regulatory molecules involved in the modulation of gene expression. Although originally assumed as non-coding RNAs, recent studies have evidenced that animal circRNAs can act as translatable transcripts. The study of plant-circRNAs is incipient, and no autonomous coding plant-circRNA has been described yet. Viroids are the smallest plant-pathogenic circRNAs known to date. Since their discovery 50 years ago, viroids have been considered valuable systems for the study of the structure-function relationships in RNA, essentially because they have not been shown to have coding capacity. We used two pathogenic circRNAs (Hop stunt viroid and Eggplant latent viroid) as experimental tools to explore the coding potential of plant-circRNAs. Our work supports that the analysed viroids contain putative ORFs able to encode peptides carrying subcellular localization signals coincident with the corresponding replication-specific organelle. Bioassays in well-established hosts revealed that mutations in these ORFs diminish their biological efficiency. Interestingly, circular forms of HSVd and ELVd were found to co-sediment with polysomes, revealing their physical interaction with the translational machinery of the plant cell. Based on this evidence we hypothesize about the possibility that plant circRNAs in general, and viroids in particular, can act, under certain cellular conditions, as non-canonical translatable transcripts.This work was supported by the Spanish Ministry of Economy and Competitiveness (co-supported by FEDER) PID2020-115571RB-I00 (VP) and AGL2016-79825-R (GG), and by the Spanish Ministry of Science and Innovation (co-supported by FEDER) Grant PID2019-104126RB-I00 (GG). JMM was the recipient of a predoctoral contract supported by the Conselleria d´Educació, Investigació, Cultura i Esport Generalitat Valenciana - ACIF Programme (ACIF-2017-114). The funders had no role in the experiment design, data analysis, decision to publish, or preparation of the manuscript; Conselleria d’Educació, Investigació, Cultura i Esport [ACIF-2017-114]; Ministerio de Economia, Industria y Competitividad, Gobierno de España [AGL2016- 79825-R]; Ministerio de Economia, Industria y Competitividad, Gobierno de España [PID2020-115571RB-I00]; inisterio de Ciencia, Innovación y Universidades [PID2019-104126RB-I00].Peer reviewedTaylor & FrancisMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionGeneralitat ValencianaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/262231reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2016-79825-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104126RB-I00https://doi.org/10.1080/15476286.2021.1962670Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2622312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Might exogenous circular RNAs act as protein-coding transcripts in plants?
title Might exogenous circular RNAs act as protein-coding transcripts in plants?
spellingShingle Might exogenous circular RNAs act as protein-coding transcripts in plants?
Marquez-Molins, Joan
Circular RNAs
Plant coding circRNAs
Non canonical transcripts
Plant pathogenic RNAs
Viroids
Viroid-derived peptides
title_short Might exogenous circular RNAs act as protein-coding transcripts in plants?
title_full Might exogenous circular RNAs act as protein-coding transcripts in plants?
title_fullStr Might exogenous circular RNAs act as protein-coding transcripts in plants?
title_full_unstemmed Might exogenous circular RNAs act as protein-coding transcripts in plants?
title_sort Might exogenous circular RNAs act as protein-coding transcripts in plants?
dc.creator.none.fl_str_mv Marquez-Molins, Joan
Navarro, José A.
Cervera-Seco, Luis
Pallás Benet, Vicente
Gómez, Gustavo
author Marquez-Molins, Joan
author_facet Marquez-Molins, Joan
Navarro, José A.
Cervera-Seco, Luis
Pallás Benet, Vicente
Gómez, Gustavo
author_role author
author2 Navarro, José A.
Cervera-Seco, Luis
Pallás Benet, Vicente
Gómez, Gustavo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Generalitat Valenciana
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Circular RNAs
Plant coding circRNAs
Non canonical transcripts
Plant pathogenic RNAs
Viroids
Viroid-derived peptides
topic Circular RNAs
Plant coding circRNAs
Non canonical transcripts
Plant pathogenic RNAs
Viroids
Viroid-derived peptides
description Circular RNAs (circRNAs) are regulatory molecules involved in the modulation of gene expression. Although originally assumed as non-coding RNAs, recent studies have evidenced that animal circRNAs can act as translatable transcripts. The study of plant-circRNAs is incipient, and no autonomous coding plant-circRNA has been described yet. Viroids are the smallest plant-pathogenic circRNAs known to date. Since their discovery 50 years ago, viroids have been considered valuable systems for the study of the structure-function relationships in RNA, essentially because they have not been shown to have coding capacity. We used two pathogenic circRNAs (Hop stunt viroid and Eggplant latent viroid) as experimental tools to explore the coding potential of plant-circRNAs. Our work supports that the analysed viroids contain putative ORFs able to encode peptides carrying subcellular localization signals coincident with the corresponding replication-specific organelle. Bioassays in well-established hosts revealed that mutations in these ORFs diminish their biological efficiency. Interestingly, circular forms of HSVd and ELVd were found to co-sediment with polysomes, revealing their physical interaction with the translational machinery of the plant cell. Based on this evidence we hypothesize about the possibility that plant circRNAs in general, and viroids in particular, can act, under certain cellular conditions, as non-canonical translatable transcripts.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/262231
url http://hdl.handle.net/10261/262231
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2016-79825-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104126RB-I00
https://doi.org/10.1080/15476286.2021.1962670
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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