Cell bioenergetics and ATP production of boar spermatozoa

Cellular metabolism is an important feature of spermatozoa that deserves more insights to be fully understood, in particular in porcine semen physiology. The present study aims to characterize the balance between glycolytic and oxidative metabolism in boar sperm cells. Agilent Seahorse technology wa...

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Detalhes bibliográficos
Autores: Blanco-Prieto, Olga, Algieri, Cristina, Spinaci, Marcella, Trombetti, Fabiana, Nesci, Salvatore, Bucci, Diego
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2023
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/468050
Acesso em linha:https://doi.org/10.1016/j.theriogenology.2023.07.018
https://hdl.handle.net/10459.1/468050
Access Level:Acceso aberto
Palavra-chave:Pig sperm cells
Mitochondria
Glycolysis
Metabolism
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spelling Cell bioenergetics and ATP production of boar spermatozoaBlanco-Prieto, OlgaAlgieri, CristinaSpinaci, MarcellaTrombetti, FabianaNesci, SalvatoreBucci, DiegoPig sperm cellsMitochondriaGlycolysisMetabolismCellular metabolism is an important feature of spermatozoa that deserves more insights to be fully understood, in particular in porcine semen physiology. The present study aims to characterize the balance between glycolytic and oxidative metabolism in boar sperm cells. Agilent Seahorse technology was used to assess both oxygen consumption rate (OCR), as an oxidative metabolism index, and extracellular acidification rate (ECAR), as a glycolytic index. Different metabolic parameters were studied on freshly ejaculated sperm cells (identified as day zero sample, d0) and after one day of storage at 17 °C in Androhep extender (d1). Mitochondrial ATP production rate (MitoATP) was higher than the glycolytic ATP production rate (glycoATP) at both d0 and d1 while at d1 the amount of ATP production decreased, in particular, due to OXPHOS reduction. Conversely, glycoATP was not significantly different between d0 and d1. Interestingly, OCR profile showed no different bioenergetic parameters (i.e. ATP turnover, basal or maximal respiration, and spare respiration) between d0 and d1, thus indicating that sperm cell metabolism was reversibly decreased by preservation conditions. Other metabolic parameters showed the same trend, irrespective of the storage time: under stressed conditions (oligomycin plus FCCP), spermatozoa showed an increase in mitochondrial respiration while the metabolic potential of glycolysis did not undergo variations when compared to baseline metabolism. The rate of oxidation of fuel substrates - glucose, fatty acids, and glutamine - showed that sperm reliance on glucose oxidation to maintain baseline respiration was higher than fatty acids or glutamine. Interestingly spermatozoa demonstrated to have a low 'capacity' parameter, which indicates that they cannot use only a single fuel substrate to produce energy. This feature of sperm metabolism to be unable to increase oxidation of a particular fuel to compensate for inhibition of alternative fuel pathway(s) was demonstrated by the negative value of 'flexibility'. Our results showed that ATP production in boar sperm cells relied on mitochondrial oxidative metabolism in freshly ejaculated cells, while, under liquid storage conditions, their oxidative metabolism decreased while the glycolysis remained constant. These results open new fields of research in the preservation techniques of boar sperm cells.This research was supported by FONDAZIONE CASSA DI RISPARMIO IN BOLOGNA Grant n° 2020.0381.Elsevier2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.theriogenology.2023.07.018https://hdl.handle.net/10459.1/468050reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.theriogenology.2023.07.018Theriogenology, 2023, vol. 210, p. 162-168cc-by, (c) Blanco-Prieto et al., 2023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/4680502026-05-29T05:05:01Z
dc.title.none.fl_str_mv Cell bioenergetics and ATP production of boar spermatozoa
title Cell bioenergetics and ATP production of boar spermatozoa
spellingShingle Cell bioenergetics and ATP production of boar spermatozoa
Blanco-Prieto, Olga
Pig sperm cells
Mitochondria
Glycolysis
Metabolism
title_short Cell bioenergetics and ATP production of boar spermatozoa
title_full Cell bioenergetics and ATP production of boar spermatozoa
title_fullStr Cell bioenergetics and ATP production of boar spermatozoa
title_full_unstemmed Cell bioenergetics and ATP production of boar spermatozoa
title_sort Cell bioenergetics and ATP production of boar spermatozoa
dc.creator.none.fl_str_mv Blanco-Prieto, Olga
Algieri, Cristina
Spinaci, Marcella
Trombetti, Fabiana
Nesci, Salvatore
Bucci, Diego
author Blanco-Prieto, Olga
author_facet Blanco-Prieto, Olga
Algieri, Cristina
Spinaci, Marcella
Trombetti, Fabiana
Nesci, Salvatore
Bucci, Diego
author_role author
author2 Algieri, Cristina
Spinaci, Marcella
Trombetti, Fabiana
Nesci, Salvatore
Bucci, Diego
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pig sperm cells
Mitochondria
Glycolysis
Metabolism
topic Pig sperm cells
Mitochondria
Glycolysis
Metabolism
description Cellular metabolism is an important feature of spermatozoa that deserves more insights to be fully understood, in particular in porcine semen physiology. The present study aims to characterize the balance between glycolytic and oxidative metabolism in boar sperm cells. Agilent Seahorse technology was used to assess both oxygen consumption rate (OCR), as an oxidative metabolism index, and extracellular acidification rate (ECAR), as a glycolytic index. Different metabolic parameters were studied on freshly ejaculated sperm cells (identified as day zero sample, d0) and after one day of storage at 17 °C in Androhep extender (d1). Mitochondrial ATP production rate (MitoATP) was higher than the glycolytic ATP production rate (glycoATP) at both d0 and d1 while at d1 the amount of ATP production decreased, in particular, due to OXPHOS reduction. Conversely, glycoATP was not significantly different between d0 and d1. Interestingly, OCR profile showed no different bioenergetic parameters (i.e. ATP turnover, basal or maximal respiration, and spare respiration) between d0 and d1, thus indicating that sperm cell metabolism was reversibly decreased by preservation conditions. Other metabolic parameters showed the same trend, irrespective of the storage time: under stressed conditions (oligomycin plus FCCP), spermatozoa showed an increase in mitochondrial respiration while the metabolic potential of glycolysis did not undergo variations when compared to baseline metabolism. The rate of oxidation of fuel substrates - glucose, fatty acids, and glutamine - showed that sperm reliance on glucose oxidation to maintain baseline respiration was higher than fatty acids or glutamine. Interestingly spermatozoa demonstrated to have a low 'capacity' parameter, which indicates that they cannot use only a single fuel substrate to produce energy. This feature of sperm metabolism to be unable to increase oxidation of a particular fuel to compensate for inhibition of alternative fuel pathway(s) was demonstrated by the negative value of 'flexibility'. Our results showed that ATP production in boar sperm cells relied on mitochondrial oxidative metabolism in freshly ejaculated cells, while, under liquid storage conditions, their oxidative metabolism decreased while the glycolysis remained constant. These results open new fields of research in the preservation techniques of boar sperm cells.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.theriogenology.2023.07.018
https://hdl.handle.net/10459.1/468050
url https://doi.org/10.1016/j.theriogenology.2023.07.018
https://hdl.handle.net/10459.1/468050
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.theriogenology.2023.07.018
Theriogenology, 2023, vol. 210, p. 162-168
dc.rights.none.fl_str_mv cc-by, (c) Blanco-Prieto et al., 2023
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by, (c) Blanco-Prieto et al., 2023
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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