Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum

This article belongs to the Section Organelle Function.

Detalhes bibliográficos
Autores: López Martín, Sergio, Pérez-Linero, Ana María, Manzano-López, Javier, Sabido-Bozo, Susana, Cortés-Gómez, Alejandro, Rodríguez-Gallardo, Sofía, Aguilera-Romero, Auxiliadora, Goder, Veit, Muñíz, Manuel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/228644
Acesso em linha:http://hdl.handle.net/10261/228644
Access Level:acceso abierto
Palavra-chave:Endoplasmic reticulum
Cargo receptors
p24 complex
Secretory cargo
Bulk flow
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spelling Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic ReticulumLópez Martín, Sergio Pérez-Linero, Ana MaríaManzano-López, JavierSabido-Bozo, SusanaCortés-Gómez, AlejandroRodríguez-Gallardo, SofíaAguilera-Romero, AuxiliadoraGoder, VeitMuñíz, ManuelEndoplasmic reticulumCargo receptorsp24 complexSecretory cargoBulk flowThis article belongs to the Section Organelle Function.The cellular mechanisms that ensure the selectivity and fidelity of secretory cargo protein transport from the endoplasmic reticulum (ER) to the Golgi are still not well understood. The p24 protein complex acts as a specific cargo receptor for GPI-anchored proteins by facilitating their ER exit through a specialized export pathway in yeast. In parallel, the p24 complex can also exit the ER using the general pathway that exports the rest of secretory proteins with their respective cargo receptors. Here, we show biochemically that the p24 complex associates at the ER with other cargo receptors in a COPII-dependent manner, forming high-molecular weight multireceptor complexes. Furthermore, live cell imaging analysis reveals that the p24 complex is required to retain in the ER secretory cargos when their specific receptors are absent. This requirement does not involve neither the unfolded protein response nor the retrograde transport from the Golgi. Our results suggest that, in addition to its role as a cargo receptor in the specialized GPI-anchored protein pathway, the p24 complex also plays an independent role in secretory cargo selectivity during its exit through the general ER export pathway, preventing the non-selective bulk flow of native secretory cargos. This mechanism would ensure receptor-regulated cargo transport, providing an additional layer of regulation of secretory cargo selectivity during ER export.This research was funded by the Spanish Ministry of Economy and Competitiveness (MINECO), BFU2017-89700-P to Manuel Muñiz and BFU2016-78265-P to Veit Goder. ‘V Own Research Plan’ of the University of Seville (VPPI-US) contract (cofounded by the European Social Fund) to Sergio Lopez. University of Seville fellowships to Sofia Rodriguez-Gallardo, Ana Maria Perez-Linero and Javier Manzano-Lopez. Ministry of Education, Culture and Sport (MECD) fellowship to Susana Sabido-Bozo.Multidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Ministerio de Economía y Competitividad (España)Universidad de SevillaEuropean CommissionMinisterio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/228644reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-89700-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-78265-Phttp://doi.org/10.3390/cells9051295Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2286442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
spellingShingle Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
López Martín, Sergio
Endoplasmic reticulum
Cargo receptors
p24 complex
Secretory cargo
Bulk flow
title_short Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_full Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_fullStr Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_full_unstemmed Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
title_sort Dual Independent Roles of the p24 Complex in Selectivity of Secretory Cargo Export from the Endoplasmic Reticulum
dc.creator.none.fl_str_mv López Martín, Sergio
Pérez-Linero, Ana María
Manzano-López, Javier
Sabido-Bozo, Susana
Cortés-Gómez, Alejandro
Rodríguez-Gallardo, Sofía
Aguilera-Romero, Auxiliadora
Goder, Veit
Muñíz, Manuel
author López Martín, Sergio
author_facet López Martín, Sergio
Pérez-Linero, Ana María
Manzano-López, Javier
Sabido-Bozo, Susana
Cortés-Gómez, Alejandro
Rodríguez-Gallardo, Sofía
Aguilera-Romero, Auxiliadora
Goder, Veit
Muñíz, Manuel
author_role author
author2 Pérez-Linero, Ana María
Manzano-López, Javier
Sabido-Bozo, Susana
Cortés-Gómez, Alejandro
Rodríguez-Gallardo, Sofía
Aguilera-Romero, Auxiliadora
Goder, Veit
Muñíz, Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Economía y Competitividad (España)
Universidad de Sevilla
European Commission
Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Endoplasmic reticulum
Cargo receptors
p24 complex
Secretory cargo
Bulk flow
topic Endoplasmic reticulum
Cargo receptors
p24 complex
Secretory cargo
Bulk flow
description This article belongs to the Section Organelle Function.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/228644
url http://hdl.handle.net/10261/228644
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-89700-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-78265-P
http://doi.org/10.3390/cells9051295
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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