Nuclear factor-kappa B in the liver of patients with chronic hepatitis C: decreased RelA expression is associated with enhanced fibrosis progression

The mechanisms of liver damage in chronic hepatitis C virus (HCV) infection are poorly understood. The transcription factor, nuclear factor-kappa B (NF-kappa B), regulates the expression of genes involved in apoptosis, inflammation, and antiviral response. It plays a protective role in several forms...

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Detalles Bibliográficos
Autores: Boya, P. (Patricia)|||/items/f7ca6760-aa78-4f66-b773-f0b54eec3919, Larrea-Leoz, E. (Esther)|||/items/37a0521f-3293-4001-85d1-bc71241d8a83, Sola, J. (Josu)|||/items/b0d68be5-9ec1-463f-a613-995f298887ab, Majano, P.L. (Pedro Lorenzo)|||/items/8dbdb316-3e6e-43cd-a56e-dc811892b47c, Jimenez, C. (Carlos)|||/items/22d4f16b-23e6-45e2-8b0e-5be59715d944, Civeira, M.P. (María Pilar)|||/items/9aa9621a-63e5-4891-b19c-686ce6f2c29b, Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71
Tipo de recurso: artículo
Fecha de publicación:2001
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/23300
Acceso en línea:https://hdl.handle.net/10171/23300
Access Level:acceso abierto
Palabra clave:Hepatitis C, Chronic/metabolism
Liver/metabolism
NF-kappa B/metabolism
Anatomía patológica
Hepatología
Descripción
Sumario:The mechanisms of liver damage in chronic hepatitis C virus (HCV) infection are poorly understood. The transcription factor, nuclear factor-kappa B (NF-kappa B), regulates the expression of genes involved in apoptosis, inflammation, and antiviral response. It plays a protective role in several forms of liver damage. In this study, we analyzed NF-kappa B by gel mobility shift assay and immunohistochemistry in liver biopsies from HCV-infected patients, and we have determined the hepatic levels of the components of the NF-kappa B system by semiquantitative polymerase chain reaction (PCR). We found that NF-kappa B was activated in the liver of patients with chronic hepatitis C. Neither NF-kappa B activity nor the RNA levels of NF-kappa B subunits showed correlation with liver inflammatory activity, viral load, or HCV genotype. By contrast, hepatic mRNA values of RelA, the main element of active NF-kappa B, correlated inversely with apoptosis (r = -.68; P <.05) and with the rate of fibrosis progression (r = -.51; P <.04). In intermediate/rapid fibrosers, RelA mRNA levels were significantly decreased as compared with slow fibrosers (P <.003) and with normal livers (P <.03). In conclusion, we found that NF-kappa B is activated in chronic HCV-infected livers, and that the expression of RelA is inversely correlated with liver cell apoptosis and with the rate of fibrosis progression. Our data thus suggest that RelA expression may protect against liver fibrosis and hepatocellular damage.

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