Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES

[EN] Background: Exposure to arsenic (As) and its metabolites can affect normal growth in children, but the combine effects at simultaneous low-level exposures, remain uncertain. Hence, this study aims to analyze how the combined effects of As and its metabolites can impact growth indicators in 1,79...

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Autores: García Villarino, Miguel, Fernández Iglesias, Rocío, García, Ana Victoria, Villa Fernández, Elsa, Fernández Arce, Lucía, Riaño Galán, Isolina, Lambert, Carmen, Martín Sánchez, Vicente, Karagas, Margaret R., Delgado Álvarez, Elías, Fernández Somoano, Ana, Signes Pastor, Antonio José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:dnet:buleria_____::6b60d110c6d593e9f7f9d3d0df502ddf
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0160412025000984?via%3Dihub
https://hdl.handle.net/10612/28066
Access Level:acceso abierto
Palabra clave:Medicina. Salud
Toxicología
Inorganic arsenic
Children
BKMR
As methylation
Mixture
3212 Salud Publica
3214 Toxicología
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spelling Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANESGarcía Villarino, MiguelFernández Iglesias, RocíoGarcía, Ana VictoriaVilla Fernández, ElsaFernández Arce, LucíaRiaño Galán, IsolinaLambert, CarmenMartín Sánchez, VicenteKaragas, Margaret R.Delgado Álvarez, ElíasFernández Somoano, AnaSignes Pastor, Antonio JoséMedicina. SaludToxicologíaInorganic arsenicChildrenBKMRAs methylationMixture3212 Salud Publica3214 Toxicología[EN] Background: Exposure to arsenic (As) and its metabolites can affect normal growth in children, but the combine effects at simultaneous low-level exposures, remain uncertain. Hence, this study aims to analyze how the combined effects of As and its metabolites can impact growth indicators in 1,792 US children aged 6–12 years, from the NHANES. Methods: Levels of arsenic species in urine were measured using HPLC coupled with ICP-DRC-MS during the 2007–2020 NHANES cycles. The sum of iAs ([AsIII + AsV]), MMA, and DMA was used as a biomarker of internal iAs exposure (∑As), and methylation efficiency was assessed using the primary and secondary methylation indices (PMI, SMI). Linear regression and BKMR models were applied to identify adverse effects, nonlinear associations, interactions, and combined effects. Results: Median concentrations of MMA, DMA, iAs, and ∑As were 0.56 μg/L, 4.07 μg/L, 1.33 μg/L, and 6.40 μg/L, respectively. In the linear regression analyses, higher urinary concentrations of MMA were associated with reductions in several growth indicators. Specifically, each interquartile range (IQR) increase in MMA concentration was linked to decreases of −0.18 (95 % CI: −0.29, −0.06) in Body Mass Index (BMI) Z-score, −0.18 (95 % CI: −0.29, −0.06) in Weight Z-score, and −0.01 (95 % CI: −0.02, −0.01) in Waist circumference/Height ratio. Additionally, higher DMA concentrations were negatively associated with Height Z-score, with a reduction of −0.08 (95 % CI: −0.15, −0.01). In the BKMR analysis, DMA consistently emerged as the dominant contributor across multiple outcomes, showing the highest Posterior Inclusion Probabilities (PIPs) for indicators such as BMI Z-score and Waist circumference/Height ratio. While MMA exhibited notable PIPs in certain models, its influence was generally weaker than that of DMA. Conclusion: Childhood exposure to a mixture of arsenic species, even at low levels, appears to influence growth indicators and adversely affect physical development in children enrolled in NHANESSIThis study has been funded by Instituto de Salud Carlos III (ISCIII) through the project PI19/01162 and co-funded by the European Union and Universidad de Oviedo. This study was also funded by grants from CIDEGENT/2020/050 (Antonio J. Signes-Pastor); P01ES022832, RD83544201, and R25CA134286 (Margaret R. Karagas)ElsevierMedicina PreventivaFacultad de Veterinaria2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.sciencedirect.com/science/article/pii/S0160412025000984?via%3Dihubhttps://hdl.handle.net/10612/28066reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/ ISCIII/ Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/ PI19http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:dnet:buleria_____::6b60d110c6d593e9f7f9d3d0df502ddf2026-06-24T12:43:27Z
dc.title.none.fl_str_mv Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
title Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
spellingShingle Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
García Villarino, Miguel
Medicina. Salud
Toxicología
Inorganic arsenic
Children
BKMR
As methylation
Mixture
3212 Salud Publica
3214 Toxicología
title_short Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
title_full Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
title_fullStr Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
title_full_unstemmed Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
title_sort Exposure to a mixture of arsenic species and growth indicators in 6–12-year-old children from the cycles 2007–2020 NHANES
dc.creator.none.fl_str_mv García Villarino, Miguel
Fernández Iglesias, Rocío
García, Ana Victoria
Villa Fernández, Elsa
Fernández Arce, Lucía
Riaño Galán, Isolina
Lambert, Carmen
Martín Sánchez, Vicente
Karagas, Margaret R.
Delgado Álvarez, Elías
Fernández Somoano, Ana
Signes Pastor, Antonio José
author García Villarino, Miguel
author_facet García Villarino, Miguel
Fernández Iglesias, Rocío
García, Ana Victoria
Villa Fernández, Elsa
Fernández Arce, Lucía
Riaño Galán, Isolina
Lambert, Carmen
Martín Sánchez, Vicente
Karagas, Margaret R.
Delgado Álvarez, Elías
Fernández Somoano, Ana
Signes Pastor, Antonio José
author_role author
author2 Fernández Iglesias, Rocío
García, Ana Victoria
Villa Fernández, Elsa
Fernández Arce, Lucía
Riaño Galán, Isolina
Lambert, Carmen
Martín Sánchez, Vicente
Karagas, Margaret R.
Delgado Álvarez, Elías
Fernández Somoano, Ana
Signes Pastor, Antonio José
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Medicina Preventiva
Facultad de Veterinaria
dc.subject.none.fl_str_mv Medicina. Salud
Toxicología
Inorganic arsenic
Children
BKMR
As methylation
Mixture
3212 Salud Publica
3214 Toxicología
topic Medicina. Salud
Toxicología
Inorganic arsenic
Children
BKMR
As methylation
Mixture
3212 Salud Publica
3214 Toxicología
description [EN] Background: Exposure to arsenic (As) and its metabolites can affect normal growth in children, but the combine effects at simultaneous low-level exposures, remain uncertain. Hence, this study aims to analyze how the combined effects of As and its metabolites can impact growth indicators in 1,792 US children aged 6–12 years, from the NHANES. Methods: Levels of arsenic species in urine were measured using HPLC coupled with ICP-DRC-MS during the 2007–2020 NHANES cycles. The sum of iAs ([AsIII + AsV]), MMA, and DMA was used as a biomarker of internal iAs exposure (∑As), and methylation efficiency was assessed using the primary and secondary methylation indices (PMI, SMI). Linear regression and BKMR models were applied to identify adverse effects, nonlinear associations, interactions, and combined effects. Results: Median concentrations of MMA, DMA, iAs, and ∑As were 0.56 μg/L, 4.07 μg/L, 1.33 μg/L, and 6.40 μg/L, respectively. In the linear regression analyses, higher urinary concentrations of MMA were associated with reductions in several growth indicators. Specifically, each interquartile range (IQR) increase in MMA concentration was linked to decreases of −0.18 (95 % CI: −0.29, −0.06) in Body Mass Index (BMI) Z-score, −0.18 (95 % CI: −0.29, −0.06) in Weight Z-score, and −0.01 (95 % CI: −0.02, −0.01) in Waist circumference/Height ratio. Additionally, higher DMA concentrations were negatively associated with Height Z-score, with a reduction of −0.08 (95 % CI: −0.15, −0.01). In the BKMR analysis, DMA consistently emerged as the dominant contributor across multiple outcomes, showing the highest Posterior Inclusion Probabilities (PIPs) for indicators such as BMI Z-score and Waist circumference/Height ratio. While MMA exhibited notable PIPs in certain models, its influence was generally weaker than that of DMA. Conclusion: Childhood exposure to a mixture of arsenic species, even at low levels, appears to influence growth indicators and adversely affect physical development in children enrolled in NHANES
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0160412025000984?via%3Dihub
https://hdl.handle.net/10612/28066
url https://www.sciencedirect.com/science/article/pii/S0160412025000984?via%3Dihub
https://hdl.handle.net/10612/28066
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ ISCIII/ Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/ PI19
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
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