Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges

Background: Microsatellite markers were developed for distylous Linum suffruticosum and tested in the monomorphic sister species Linum tenuifolium. These species are perennial herbs endemic to the western and northwestern Mediterranean, respectively, with a partially overlapping distribution area. M...

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Autores: Olmedo Vicente, Erika, Désamoré, Aurélie, Simón Porcar, Violeta, Slotte, Tanja, Arroyo Marín, Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/149233
Acceso en línea:https://hdl.handle.net/11441/149233
https://doi.org/10.1007/s11033-023-08471-9
Access Level:acceso abierto
Palabra clave:Floral polymorphism
Genetic variation
Heterostyly
Hybrid zones
Linum
Microsatellites
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spelling Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping rangesOlmedo Vicente, ErikaDésamoré, AurélieSimón Porcar, VioletaSlotte, TanjaArroyo Marín, JuanFloral polymorphismGenetic variationHeterostylyHybrid zonesLinumMicrosatellitesBackground: Microsatellite markers were developed for distylous Linum suffruticosum and tested in the monomorphic sister species Linum tenuifolium. These species are perennial herbs endemic to the western and northwestern Mediterranean, respectively, with a partially overlapping distribution area. Methods and results: We developed 12 microsatellite markers for L. suffruticosum using next generation sequencing, and assessed their polymorphism and genetic diversity in 152 individuals from seven natural populations. The markers displayed high polymorphism, with two to 16 alleles per locus and population, and average observed and expected heterozygosities of 0.833 and 0.692, respectively. All loci amplified successfully in the sister species L. tenuifolium, and 150 individuals from seven populations were also screened. The polymorphism exhibited was high, with two to ten alleles per locus and population, and average observed and expected heterozygosities of 0.77 and 0.62, respectively. Conclusions: The microsatellite markers identified in L. suffruticosum and tested in L. tenuifolium are a powerful tool to facilitate future investigations of the population genetics, mating patterns and hybridization between both Linum species in their contact zone.European Research Council 757451Ministerio de Ciencia e Innovación CGL2013-45037-P, PGC2018 099608 B 100, PID2021-122715NB-I00European Union 897890Springer NatureBiología Vegetal y EcologíaEuropean Research Council (ERC)Ministerio de Ciencia e Innovación (MICIN). EspañaEuropean Union (UE)2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/149233https://doi.org/10.1007/s11033-023-08471-9reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Biology Reports, 50 (9), 7927-7933.757451CGL2013-45037-PPGC2018 099608 B 100PID2021-122715NB-I00897890https://doi.org/10.1007/s11033-023-08471-9info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1492332026-06-17T12:51:07Z
dc.title.none.fl_str_mv Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
title Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
spellingShingle Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
Olmedo Vicente, Erika
Floral polymorphism
Genetic variation
Heterostyly
Hybrid zones
Linum
Microsatellites
title_short Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
title_full Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
title_fullStr Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
title_full_unstemmed Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
title_sort Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
dc.creator.none.fl_str_mv Olmedo Vicente, Erika
Désamoré, Aurélie
Simón Porcar, Violeta
Slotte, Tanja
Arroyo Marín, Juan
author Olmedo Vicente, Erika
author_facet Olmedo Vicente, Erika
Désamoré, Aurélie
Simón Porcar, Violeta
Slotte, Tanja
Arroyo Marín, Juan
author_role author
author2 Désamoré, Aurélie
Simón Porcar, Violeta
Slotte, Tanja
Arroyo Marín, Juan
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Biología Vegetal y Ecología
European Research Council (ERC)
Ministerio de Ciencia e Innovación (MICIN). España
European Union (UE)
dc.subject.none.fl_str_mv Floral polymorphism
Genetic variation
Heterostyly
Hybrid zones
Linum
Microsatellites
topic Floral polymorphism
Genetic variation
Heterostyly
Hybrid zones
Linum
Microsatellites
description Background: Microsatellite markers were developed for distylous Linum suffruticosum and tested in the monomorphic sister species Linum tenuifolium. These species are perennial herbs endemic to the western and northwestern Mediterranean, respectively, with a partially overlapping distribution area. Methods and results: We developed 12 microsatellite markers for L. suffruticosum using next generation sequencing, and assessed their polymorphism and genetic diversity in 152 individuals from seven natural populations. The markers displayed high polymorphism, with two to 16 alleles per locus and population, and average observed and expected heterozygosities of 0.833 and 0.692, respectively. All loci amplified successfully in the sister species L. tenuifolium, and 150 individuals from seven populations were also screened. The polymorphism exhibited was high, with two to ten alleles per locus and population, and average observed and expected heterozygosities of 0.77 and 0.62, respectively. Conclusions: The microsatellite markers identified in L. suffruticosum and tested in L. tenuifolium are a powerful tool to facilitate future investigations of the population genetics, mating patterns and hybridization between both Linum species in their contact zone.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/149233
https://doi.org/10.1007/s11033-023-08471-9
url https://hdl.handle.net/11441/149233
https://doi.org/10.1007/s11033-023-08471-9
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Biology Reports, 50 (9), 7927-7933.
757451
CGL2013-45037-P
PGC2018 099608 B 100
PID2021-122715NB-I00
897890
https://doi.org/10.1007/s11033-023-08471-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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