Effect of eicosapentaenoic acid-derived prostaglandin E3 on intestinal epitelial barrier function
<p>Prostaglandins (PG) are inflammatory mediators derived from arachidonic or eicosapentaenoic acid giving rise to the 2-series or the 3-series prostanoids, respectively. Previously, we have observed that PGE 2 disrupts epithelial barrier function. Considering the beneficial effect of fish oil...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/207865 |
| Acceso en línea: | https://hdl.handle.net/2445/207865 |
| Access Level: | acceso abierto |
| Palabra clave: | Malalties inflamatòries intestinals Epiteli Cèl·lules epitelials Inflammatory bowel diseases Epithelium Epithelial cells |
| Sumario: | <p>Prostaglandins (PG) are inflammatory mediators derived from arachidonic or eicosapentaenoic acid giving rise to the 2-series or the 3-series prostanoids, respectively. Previously, we have observed that PGE 2 disrupts epithelial barrier function. Considering the beneficial effect of fish oil consumption in intestinal inflammatory processes, the aim of this study was to assess the role of PGE 3 on epithelial barrier function assessed from transepithelial electrical resistance and dextran fluxes in Caco-2 cells. The results indicate that PGE 3 increased paracellular permeability (PP) to the same extent as PGE 2, through the interaction with EP 1 and EP 4 receptors and with intracellular Ca 2+ and cAMP as the downstream targets. Moreover, we observed a redistribution of tight junction proteins, occludin and claudin-4. In conclusion, PGE 3 is able to increase PP thus leading to reconsider the role of PGE 2/PGE 3 ratio in the beneficial effects of dietary fish oil supplementation in the disruption of barrier function.</p> |
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