Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes

Type 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte-endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition...

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Autores: Iannantuoni F, M de Marañon A, Abad-Jiménez Z, Canet F, Díaz-Pozo P, López-Domènech S, Morillas C, Rocha M, Víctor VM
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p7808
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/7808
Access Level:acceso abierto
Palabra clave:type 1 diabetes
mitochondria
endothelium
inflammation
cardiovascular risk
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spelling Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 DiabetesIannantuoni FM de Marañon AAbad-Jiménez ZCanet FDíaz-Pozo PLópez-Domènech SMorillas CRocha MVíctor VMtype 1 diabetesmitochondriaendotheliuminflammationcardiovascular riskType 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte-endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition and age-matched controls. We assessed anthropometric and metabolic parameters, oxidative stress and mitochondrial function by evaluating reactive oxygen species (ROS) production, mitochondrial ROS production, mitochondrial membrane potential and superoxide dismutase (SOD) and catalase (CAT) expression in polymorphonuclear leukocytes from type 1 diabetic patients. In addition, we evaluated interactions between leukocytes and human umbilical vein endothelial cells (HUVEC), and serum expression of adhesion molecules (P-selectin, VCAM-1 and ICAM-1), proinflammatory cytokines (IL-6 and TNF alpha) and myeloperoxidase (MPO). HbA(1C)and glucose levels were higher in diabetic patients than in control subjects, as expected. Mitochondrial function was altered and leukocyte-endothelium interactions were enhanced in diabetic patients, which was evident in the increase in total and mitochondrial ROS production, higher mitochondrial membrane potential, enhanced leukocyte rolling and adhesion, and decreased rolling velocity. Furthermore, we observed an increase in levels of adhesion molecules P-selectin, VCAM-1, and ICAM-1 in these subjects. In addition, type 1 diabetic patients exhibited an increase in proinflammatory mediators TNF alpha and MPO, and a decreased expression of SOD. The enhancement of leukocyte-endothelium interactions and proinflammatory markers correlated with glucose and HbA(1C)levels. Mitochondrial alteration, oxidative stress, and enhanced leukocyte-endothelium interactions are features of type 1 diabetes and may be related to cardiovascular implications.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/7808Journal of Clinical MedicineISSN: 20770383reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p78082026-06-11T12:45:17Z
dc.title.none.fl_str_mv Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
spellingShingle Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
Iannantuoni F
type 1 diabetes
mitochondria
endothelium
inflammation
cardiovascular risk
title_short Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_full Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_fullStr Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_full_unstemmed Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
title_sort Mitochondrial Alterations and Enhanced Human Leukocyte/Endothelial Cell Interactions in Type 1 Diabetes
dc.creator.none.fl_str_mv Iannantuoni F
M de Marañon A
Abad-Jiménez Z
Canet F
Díaz-Pozo P
López-Domènech S
Morillas C
Rocha M
Víctor VM
author Iannantuoni F
author_facet Iannantuoni F
M de Marañon A
Abad-Jiménez Z
Canet F
Díaz-Pozo P
López-Domènech S
Morillas C
Rocha M
Víctor VM
author_role author
author2 M de Marañon A
Abad-Jiménez Z
Canet F
Díaz-Pozo P
López-Domènech S
Morillas C
Rocha M
Víctor VM
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv type 1 diabetes
mitochondria
endothelium
inflammation
cardiovascular risk
topic type 1 diabetes
mitochondria
endothelium
inflammation
cardiovascular risk
description Type 1 diabetes has been associated with oxidative stress. This study evaluates the rates of oxidative stress, mitochondrial function, leukocyte-endothelium interactions and adhesion molecules in type 1 diabetic patients. The study population consisted of 52 diabetic patients and 46 body-composition and age-matched controls. We assessed anthropometric and metabolic parameters, oxidative stress and mitochondrial function by evaluating reactive oxygen species (ROS) production, mitochondrial ROS production, mitochondrial membrane potential and superoxide dismutase (SOD) and catalase (CAT) expression in polymorphonuclear leukocytes from type 1 diabetic patients. In addition, we evaluated interactions between leukocytes and human umbilical vein endothelial cells (HUVEC), and serum expression of adhesion molecules (P-selectin, VCAM-1 and ICAM-1), proinflammatory cytokines (IL-6 and TNF alpha) and myeloperoxidase (MPO). HbA(1C)and glucose levels were higher in diabetic patients than in control subjects, as expected. Mitochondrial function was altered and leukocyte-endothelium interactions were enhanced in diabetic patients, which was evident in the increase in total and mitochondrial ROS production, higher mitochondrial membrane potential, enhanced leukocyte rolling and adhesion, and decreased rolling velocity. Furthermore, we observed an increase in levels of adhesion molecules P-selectin, VCAM-1, and ICAM-1 in these subjects. In addition, type 1 diabetic patients exhibited an increase in proinflammatory mediators TNF alpha and MPO, and a decreased expression of SOD. The enhancement of leukocyte-endothelium interactions and proinflammatory markers correlated with glucose and HbA(1C)levels. Mitochondrial alteration, oxidative stress, and enhanced leukocyte-endothelium interactions are features of type 1 diabetes and may be related to cardiovascular implications.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/7808
url https://fisabio.portalinvestigacion.com/publicaciones/7808
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Journal of Clinical Medicine
ISSN: 20770383
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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