Halomonas Avicenniae sp. nov., Isolated from the Salty Leaves of the Black Mangrove Avicennia Germinans in Puerto Rico

A Gram-negative, short rod to oval-shaped bacterium (strain MW2aT) was isolated from the surface of leaves of the black mangrove Avicennia germinans and subjected to a polyphasic taxonomic study. Strain MW2aT was moderately halophilic, growing at NaCl concentrations in the range 0-25% (w/v) with opt...

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Detalles Bibliográficos
Autores: Soto Ramírez, Nelís, Sánchez-Porro Álvarez, Cristina, Rosas, Soniris, González, Wildaomaris, Quiñones, Marian, Ventosa Ucero, Antonio, Montalvo Rodríguez, Rafael
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2007
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/165221
Acceso en línea:https://hdl.handle.net/11441/165221
https://doi.org/10.1099/ijs.0.64818-0
Access Level:acceso abierto
Descripción
Sumario:A Gram-negative, short rod to oval-shaped bacterium (strain MW2aT) was isolated from the surface of leaves of the black mangrove Avicennia germinans and subjected to a polyphasic taxonomic study. Strain MW2aT was moderately halophilic, growing at NaCl concentrations in the range 0-25% (w/v) with optimum growth at 5% (w/v) NaCl. Growth occurred at 12-40°C (optimum, 30-35°C) and at pH 5.0-9.0 (optimum, pH 7.0-8.0). Strain MW2aT was strictly aerobic. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Halomonas. The closest relative was Halomonas marisflavi, with 98.6% 16S rRNA gene sequence similarity. The DNA G + C content of strain MW2aT was 61.5 mol%, which is in the range of values for Halomonas species. DNA-DNA hybridization with H. marisflavi showed a relatedness of 42% and lower values were obtained with respect to other related Halomonas species. The major fatty acids were C16:0, C19:0 cyclo ω8c, C18:1 ω7c and C12:0 3-OH. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain Nw2aT represents a novel species within the genus Halomonas. The name Halomonas avicenniae sp. nov. is proposed, with strain MW2aT (= CECT 7193T =CCM 7396T) as the type strain.

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