Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae

Streptococcus pneumoniae is the leading cause of vaccine-preventable deaths globally. The objective of this study was to determine the distribution and clonal type variability of three potential vaccine antigens: Pneumococcal serine-rich repeat protein (PsrP), Pilus-1, and Pneumococcal choline bindi...

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Autores: Selva Jové, Laura, Ciruela, Pilar, Blanchette, Krystle, Amo Moran, Eva del, Pallarés Giner, Roman, Orihuela, Carlos J., Muñoz-Almagro, Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/101480
Acceso en línea:https://hdl.handle.net/2445/101480
Access Level:acceso abierto
Palabra clave:Pneumococs
Infeccions per pneumococs
Vacuna antipneumocòccica
Vacunes
Vacunació dels infants
Resistència als medicaments
Pediatria
Streptococcus pneumonia
Pneumococcal Infections
Pneumococcal vaccine
Vaccines
Vaccination of children
Drug resistance
Pediatrics
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spelling Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniaeSelva Jové, LauraCiruela, PilarBlanchette, KrystleAmo Moran, Eva delPallarés Giner, RomanOrihuela, Carlos J.Muñoz-Almagro, CarmenPneumococsInfeccions per pneumococsVacuna antipneumocòccicaVacunesVacunació dels infantsResistència als medicamentsPediatriaStreptococcus pneumoniaPneumococcal InfectionsPneumococcal vaccineVaccinesVaccination of childrenDrug resistancePediatricsStreptococcus pneumoniae is the leading cause of vaccine-preventable deaths globally. The objective of this study was to determine the distribution and clonal type variability of three potential vaccine antigens: Pneumococcal serine-rich repeat protein (PsrP), Pilus-1, and Pneumococcal choline binding protein A (PcpA) among pneumococcal isolates from children with invasive pneumococcal disease and healthy nasopharyngeal carriers. We studied by Real-Time PCR a total of 458 invasive pneumococcal isolates and 89 nasopharyngeal pneumococcal isolates among children (total = 547 strains) collected in Barcelona, Spain, from January 2004 to July 2010. pcpA, psrP and pilus-1 were detected in 92.8%, 51.7% and 14.4% of invasive isolates and in 92.1%, 48.3% and 18% of carrier isolates, respectively. Within individual serotypes the prevalence of psrP and pilus-1 was highly dependent on the clonal type. pcpA was highly prevalent in all strains with the exception of those belonging to serotype 3 (33.3% in serotype 3 isolates vs. 95.1% in other serotypes; P<.001). psrP was significantly more frequent in those serotypes that are less apt to be detected in carriage than in disease; 58.7% vs. 39.1% P<.001. Antibiotic resistance was associated with the presence of pilus-1 and showed a negative correlation with psrP. These results indicate that PcpA, and subsequently Psrp and Pilus-1 together might be good candidates to be used in a next-generation of multivalent pneumococcal protein vaccine.Public Library of Science (PLoS)2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/101480Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0041587PLoS One, 2012, vol. 7, num. 7, p. e41587http://dx.doi.org/10.1371/journal.pone.0041587cc-by (c) Selva Jové, Laura et al., 2012http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1014802026-05-27T06:46:51Z
dc.title.none.fl_str_mv Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
title Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
spellingShingle Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
Selva Jové, Laura
Pneumococs
Infeccions per pneumococs
Vacuna antipneumocòccica
Vacunes
Vacunació dels infants
Resistència als medicaments
Pediatria
Streptococcus pneumonia
Pneumococcal Infections
Pneumococcal vaccine
Vaccines
Vaccination of children
Drug resistance
Pediatrics
title_short Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
title_full Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
title_fullStr Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
title_full_unstemmed Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
title_sort Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae
dc.creator.none.fl_str_mv Selva Jové, Laura
Ciruela, Pilar
Blanchette, Krystle
Amo Moran, Eva del
Pallarés Giner, Roman
Orihuela, Carlos J.
Muñoz-Almagro, Carmen
author Selva Jové, Laura
author_facet Selva Jové, Laura
Ciruela, Pilar
Blanchette, Krystle
Amo Moran, Eva del
Pallarés Giner, Roman
Orihuela, Carlos J.
Muñoz-Almagro, Carmen
author_role author
author2 Ciruela, Pilar
Blanchette, Krystle
Amo Moran, Eva del
Pallarés Giner, Roman
Orihuela, Carlos J.
Muñoz-Almagro, Carmen
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Pneumococs
Infeccions per pneumococs
Vacuna antipneumocòccica
Vacunes
Vacunació dels infants
Resistència als medicaments
Pediatria
Streptococcus pneumonia
Pneumococcal Infections
Pneumococcal vaccine
Vaccines
Vaccination of children
Drug resistance
Pediatrics
topic Pneumococs
Infeccions per pneumococs
Vacuna antipneumocòccica
Vacunes
Vacunació dels infants
Resistència als medicaments
Pediatria
Streptococcus pneumonia
Pneumococcal Infections
Pneumococcal vaccine
Vaccines
Vaccination of children
Drug resistance
Pediatrics
description Streptococcus pneumoniae is the leading cause of vaccine-preventable deaths globally. The objective of this study was to determine the distribution and clonal type variability of three potential vaccine antigens: Pneumococcal serine-rich repeat protein (PsrP), Pilus-1, and Pneumococcal choline binding protein A (PcpA) among pneumococcal isolates from children with invasive pneumococcal disease and healthy nasopharyngeal carriers. We studied by Real-Time PCR a total of 458 invasive pneumococcal isolates and 89 nasopharyngeal pneumococcal isolates among children (total = 547 strains) collected in Barcelona, Spain, from January 2004 to July 2010. pcpA, psrP and pilus-1 were detected in 92.8%, 51.7% and 14.4% of invasive isolates and in 92.1%, 48.3% and 18% of carrier isolates, respectively. Within individual serotypes the prevalence of psrP and pilus-1 was highly dependent on the clonal type. pcpA was highly prevalent in all strains with the exception of those belonging to serotype 3 (33.3% in serotype 3 isolates vs. 95.1% in other serotypes; P<.001). psrP was significantly more frequent in those serotypes that are less apt to be detected in carriage than in disease; 58.7% vs. 39.1% P<.001. Antibiotic resistance was associated with the presence of pilus-1 and showed a negative correlation with psrP. These results indicate that PcpA, and subsequently Psrp and Pilus-1 together might be good candidates to be used in a next-generation of multivalent pneumococcal protein vaccine.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/101480
url https://hdl.handle.net/2445/101480
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0041587
PLoS One, 2012, vol. 7, num. 7, p. e41587
http://dx.doi.org/10.1371/journal.pone.0041587
dc.rights.none.fl_str_mv cc-by (c) Selva Jové, Laura et al., 2012
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Selva Jové, Laura et al., 2012
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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