Alu RNA modulates the expression of cell cycle genes in human fibroblasts

Alu retroelements, whose retrotransposition requires prior transcription by RNA polymerase III to generate Alu RNAs, represent the most numerous non-coding RNA (ncRNA) gene family in the human genome. Alu transcription is generally kept to extremely low levels by tight epigenetic silencing, but it h...

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Detalles Bibliográficos
Autores: Cantarella, Simona, Carnevali, Davide, Morselli, Marco, Conti, Anastasia, Pellegrini, Matteo, Montanini, Barbara, Dieci, Giorgio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/44060
Acceso en línea:http://hdl.handle.net/10230/44060
http://dx.doi.org/10.3390/ijms20133315
Access Level:acceso abierto
Palabra clave:Alu retrotransposons
Cell cycle
Non-coding RNA
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spelling Alu RNA modulates the expression of cell cycle genes in human fibroblastsCantarella, SimonaCarnevali, DavideMorselli, MarcoConti, AnastasiaPellegrini, MatteoMontanini, BarbaraDieci, GiorgioAlu retrotransposonsCell cycleNon-coding RNAAlu retroelements, whose retrotransposition requires prior transcription by RNA polymerase III to generate Alu RNAs, represent the most numerous non-coding RNA (ncRNA) gene family in the human genome. Alu transcription is generally kept to extremely low levels by tight epigenetic silencing, but it has been reported to increase under different types of cell perturbation, such as viral infection and cancer. Alu RNAs, being able to act as gene expression modulators, may be directly involved in the mechanisms determining cellular behavior in such perturbed states. To directly address the regulatory potential of Alu RNAs, we generated IMR90 fibroblasts and HeLa cell lines stably overexpressing two slightly different Alu RNAs, and analyzed genome-wide the expression changes of protein-coding genes through RNA-sequencing. Among the genes that were upregulated or downregulated in response to Alu overexpression in IMR90, but not in HeLa cells, we found a highly significant enrichment of pathways involved in cell cycle progression and mitotic entry. Accordingly, Alu overexpression was found to promote transition from G1 to S phase, as revealed by flow cytometry. Therefore, increased Alu RNA may contribute to sustained cell proliferation, which is an important factor of cancer development and progression.MDPI202020202019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/44060http://dx.doi.org/10.3390/ijms20133315reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInt J Mol Sci. 2019; 20(13). pii: E3315© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/440602026-05-29T05:05:01Z
dc.title.none.fl_str_mv Alu RNA modulates the expression of cell cycle genes in human fibroblasts
title Alu RNA modulates the expression of cell cycle genes in human fibroblasts
spellingShingle Alu RNA modulates the expression of cell cycle genes in human fibroblasts
Cantarella, Simona
Alu retrotransposons
Cell cycle
Non-coding RNA
title_short Alu RNA modulates the expression of cell cycle genes in human fibroblasts
title_full Alu RNA modulates the expression of cell cycle genes in human fibroblasts
title_fullStr Alu RNA modulates the expression of cell cycle genes in human fibroblasts
title_full_unstemmed Alu RNA modulates the expression of cell cycle genes in human fibroblasts
title_sort Alu RNA modulates the expression of cell cycle genes in human fibroblasts
dc.creator.none.fl_str_mv Cantarella, Simona
Carnevali, Davide
Morselli, Marco
Conti, Anastasia
Pellegrini, Matteo
Montanini, Barbara
Dieci, Giorgio
author Cantarella, Simona
author_facet Cantarella, Simona
Carnevali, Davide
Morselli, Marco
Conti, Anastasia
Pellegrini, Matteo
Montanini, Barbara
Dieci, Giorgio
author_role author
author2 Carnevali, Davide
Morselli, Marco
Conti, Anastasia
Pellegrini, Matteo
Montanini, Barbara
Dieci, Giorgio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Alu retrotransposons
Cell cycle
Non-coding RNA
topic Alu retrotransposons
Cell cycle
Non-coding RNA
description Alu retroelements, whose retrotransposition requires prior transcription by RNA polymerase III to generate Alu RNAs, represent the most numerous non-coding RNA (ncRNA) gene family in the human genome. Alu transcription is generally kept to extremely low levels by tight epigenetic silencing, but it has been reported to increase under different types of cell perturbation, such as viral infection and cancer. Alu RNAs, being able to act as gene expression modulators, may be directly involved in the mechanisms determining cellular behavior in such perturbed states. To directly address the regulatory potential of Alu RNAs, we generated IMR90 fibroblasts and HeLa cell lines stably overexpressing two slightly different Alu RNAs, and analyzed genome-wide the expression changes of protein-coding genes through RNA-sequencing. Among the genes that were upregulated or downregulated in response to Alu overexpression in IMR90, but not in HeLa cells, we found a highly significant enrichment of pathways involved in cell cycle progression and mitotic entry. Accordingly, Alu overexpression was found to promote transition from G1 to S phase, as revealed by flow cytometry. Therefore, increased Alu RNA may contribute to sustained cell proliferation, which is an important factor of cancer development and progression.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/44060
http://dx.doi.org/10.3390/ijms20133315
url http://hdl.handle.net/10230/44060
http://dx.doi.org/10.3390/ijms20133315
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Int J Mol Sci. 2019; 20(13). pii: E3315
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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repository.mail.fl_str_mv
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