Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats

Two milliliters of a fermented, pasteurized orange juice containing 1% alcohol and 2.3 lmol of (poly)phenolic compounds was fed to rats by gavage after which plasma and urine collected over a 36 h period were analyzed by UHPLCmass spectrometry. The main constituents in the juice were hesperetin and...

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Autores: Escudero López, Blanca, Calani, Luca, Fernández-Pachón, María-Soledad, Ortega de la Torre, María de los Ángeles, Brighenti, Furio, Crozier, Alan, Del Rio, Daniele
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad Pablo de Olavide (UPO)
Repositorio:RIO. Repositorio Institucional Olavide
Idioma:inglés
OAI Identifier:oai:rio.upo.es:10433/19608
Acceso en línea:https://hdl.handle.net/10433/19608
Access Level:acceso abierto
Palabra clave:Orange juice
Flavanones
Hydroxycinnamates
Bioavailability
Rats
Colon
Microbiota
Urinary excretion
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spelling Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in ratsEscudero López, BlancaCalani, LucaFernández-Pachón, María-SoledadOrtega de la Torre, María de los ÁngelesBrighenti, FurioCrozier, AlanDel Rio, DanieleOrange juiceFlavanonesHydroxycinnamatesBioavailabilityRatsColonMicrobiotaUrinary excretionTwo milliliters of a fermented, pasteurized orange juice containing 1% alcohol and 2.3 lmol of (poly)phenolic compounds was fed to rats by gavage after which plasma and urine collected over a 36 h period were analyzed by UHPLCmass spectrometry. The main constituents in the juice were hesperetin and naringenin-O-glycosides, apigenin-6,8-C-diglucoside, and ferulic acid-40-O-glucoside. Plasma contained seven flavanone glucuronides, with the principal metabolites, naringenin-7-O-glucuronide, naringenin-40 -O-glucuronide, and an isosakuranetin-O-glucuronide, peaking 6 h after intake at concentrations of 10 nmol/L. Urinary excretion of four hesperetin glucuronides was equivalent to 0.28% of intake while that of the two naringenin glucuronides was 2.8% of intake. The plasma and urine data suggest that while some absorption occurred in the small intestine, the main site of uptake was the colon. Urine also contained dihydroferulic acid-4’-Oglucuronide and dihydroferulic acid-40-O-sulfate which were excreted in quantities corresponding to 48.2% of the ingested ferulic acid-40-glucoside. This indicates that the hydroxycinnamate is much more bioavailable than the flavanones in the rat model. Conversion of the ferulic acid glucoside to the dihydroferulic acid metabolites involves the action of colonic microbial glycosidases and reductases/hydrogenases followed by postabsorption phase II metabolism before renal excretion.John Wiley & Sons20242024-02-0220132013-11-2020132013-11-20journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10433/19608reponame:RIO. Repositorio Institucional Olavideinstname:Universidad Pablo de Olavide (UPO)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:rio.upo.es:10433/196082026-06-13T12:46:27Z
dc.title.none.fl_str_mv Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
title Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
spellingShingle Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
Escudero López, Blanca
Orange juice
Flavanones
Hydroxycinnamates
Bioavailability
Rats
Colon
Microbiota
Urinary excretion
title_short Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
title_full Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
title_fullStr Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
title_full_unstemmed Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
title_sort Absorption, metabolism, and excretion of fermented orange juice (poly)phenols in rats
dc.creator.none.fl_str_mv Escudero López, Blanca
Calani, Luca
Fernández-Pachón, María-Soledad
Ortega de la Torre, María de los Ángeles
Brighenti, Furio
Crozier, Alan
Del Rio, Daniele
author Escudero López, Blanca
author_facet Escudero López, Blanca
Calani, Luca
Fernández-Pachón, María-Soledad
Ortega de la Torre, María de los Ángeles
Brighenti, Furio
Crozier, Alan
Del Rio, Daniele
author_role author
author2 Calani, Luca
Fernández-Pachón, María-Soledad
Ortega de la Torre, María de los Ángeles
Brighenti, Furio
Crozier, Alan
Del Rio, Daniele
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Orange juice
Flavanones
Hydroxycinnamates
Bioavailability
Rats
Colon
Microbiota
Urinary excretion
topic Orange juice
Flavanones
Hydroxycinnamates
Bioavailability
Rats
Colon
Microbiota
Urinary excretion
description Two milliliters of a fermented, pasteurized orange juice containing 1% alcohol and 2.3 lmol of (poly)phenolic compounds was fed to rats by gavage after which plasma and urine collected over a 36 h period were analyzed by UHPLCmass spectrometry. The main constituents in the juice were hesperetin and naringenin-O-glycosides, apigenin-6,8-C-diglucoside, and ferulic acid-40-O-glucoside. Plasma contained seven flavanone glucuronides, with the principal metabolites, naringenin-7-O-glucuronide, naringenin-40 -O-glucuronide, and an isosakuranetin-O-glucuronide, peaking 6 h after intake at concentrations of 10 nmol/L. Urinary excretion of four hesperetin glucuronides was equivalent to 0.28% of intake while that of the two naringenin glucuronides was 2.8% of intake. The plasma and urine data suggest that while some absorption occurred in the small intestine, the main site of uptake was the colon. Urine also contained dihydroferulic acid-4’-Oglucuronide and dihydroferulic acid-40-O-sulfate which were excreted in quantities corresponding to 48.2% of the ingested ferulic acid-40-glucoside. This indicates that the hydroxycinnamate is much more bioavailable than the flavanones in the rat model. Conversion of the ferulic acid glucoside to the dihydroferulic acid metabolites involves the action of colonic microbial glycosidases and reductases/hydrogenases followed by postabsorption phase II metabolism before renal excretion.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-11-20
2013
2013-11-20
2024
2024-02-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10433/19608
url https://hdl.handle.net/10433/19608
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:RIO. Repositorio Institucional Olavide
instname:Universidad Pablo de Olavide (UPO)
instname_str Universidad Pablo de Olavide (UPO)
reponame_str RIO. Repositorio Institucional Olavide
collection RIO. Repositorio Institucional Olavide
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403701517484032
score 15,300719

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