Relocation of the NIb gene in the tobacco etch potyvirus genome
[EN] Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all poss...
| Autores: | , , , , , |
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| Tipo de documento: | artigo |
| Data de publicação: | 2014 |
| País: | España |
| Recursos: | Universitat Politècnica de València (UPV) |
| Repositório: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglês |
| OAI Identifier: | oai:riunet.upv.es:10251/63481 |
| Acesso em linha: | https://riunet.upv.es/handle/10251/63481 |
| Access Level: | Acceso aberto |
| Palavra-chave: | VPG-PROTEINASE NIA HETEROLOGOUS PROTEINS VIRUS GENOME EXPRESSION STABILITY VECTOR PLANTS INSERTION MUTANTS PURIFICATION |
| Resumo: | [EN] Potyviruses express most of their proteins from a long open reading frame that is translated into a large polyprotein processed by three viral proteases. To understand the constraints on potyvirus genome organization, we relocated the viral RNA-dependent RNA polymerase (NIb) cistron to all possible intercistronic positions of the Tobacco etch virus (TEV) polyprotein. Only viruses with NIb at the amino terminus of the polyprotein or in between P1 and HC-Pro were viable in tobacco plants. |
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