Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry

Artificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze-dried dairy cattle feces were used (external archaea) as co-inoculum (Co-i) and structural carbohydrates (CHO: apple pulp; sugar beet pulp; and wheat straw) as...

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Autores: Morazán Nuñez, Henris Jobany, Seradj, Ahmad Reza, Álvarez Rodríguez, Javier, Abecia, Leticia, Babot Gaspa, Daniel, Yañez-Ruiz, David R., Balcells Terés, Joaquim
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/67635
Acesso em linha:https://doi.org/10.1002/ep.11952
http://hdl.handle.net/10459.1/67635
Access Level:acceso abierto
Palavra-chave:Archaea
Biodiversity
Methane
Slurry
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spelling Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurryMorazán Nuñez, Henris JobanySeradj, Ahmad RezaÁlvarez Rodríguez, JavierAbecia, LeticiaBabot Gaspa, DanielYañez-Ruiz, David R.Balcells Terés, JoaquimArchaeaBiodiversityMethaneSlurryArtificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze-dried dairy cattle feces were used (external archaea) as co-inoculum (Co-i) and structural carbohydrates (CHO: apple pulp; sugar beet pulp; and wheat straw) as substrates. Bottles were incubated (39 +/- 1 degrees C for 56 days), and the gas production was measured (mbar) and converted to the volume. A sample of produced gas was taken and analyzed for methane concentration using a gas chromatography. Bottles were opened at days 0, 25, and 56 to determine total bacteria, total, and hydrogenotrophic methanogens archaea (HMA) concentrations using quantitative polymerase chain reaction and population biodiversity using denaturing gradient gel electrophoresis (DGGE). Incubation time reduced the titers of total bacteria and archaea (P<0.01) but did not modify HMA population. Doses of Co-i showed a positive correlation with HMA titers, although interacted with an incubation period (P<0.001); at 5% Co-inoculation, total bacteria decreased significantly (0-25 days) but remained steady until day 56 (P>0.05), whereas at 10% Co inoculation, titers decreased constantly. Most of the archaeal DGGE bands were observed in all samples, suggesting a common microbial population origin but Co-i supply altered the DGGE structure of archaea populationsThis work was supported by the Ministry of Economy and Competitiveness of Spain and the European Union Regional Development Funds (AGL2010-20820). Ahmad Reza Seradj is a recipient of grant from the Government of Catalunya (FI-DGR 2011) and Henris Morazan was a recipient of grant from MAEC-AECID 2009–2011.American Institute of Chemical Engineers (AIChE)2019201920152019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1002/ep.11952http://hdl.handle.net/10459.1/67635http://hdl.handle.net/10459.1/67635reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MICINN//AGL2010-20820Versió postprint del document publicat a: https://doi.org/10.1002/ep.11952Environmental Progress & Sustainable Energy, 2015, vol. 34, num. 1, p. 54-64(c) American Institute of Chemical Engineers, 2015info:eu-repo/semantics/openAccessoai:recercat.cat:10459.1/676352026-05-29T05:05:01Z
dc.title.none.fl_str_mv Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
spellingShingle Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
Morazán Nuñez, Henris Jobany
Archaea
Biodiversity
Methane
Slurry
title_short Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_full Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_fullStr Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_full_unstemmed Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
title_sort Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
dc.creator.none.fl_str_mv Morazán Nuñez, Henris Jobany
Seradj, Ahmad Reza
Álvarez Rodríguez, Javier
Abecia, Leticia
Babot Gaspa, Daniel
Yañez-Ruiz, David R.
Balcells Terés, Joaquim
author Morazán Nuñez, Henris Jobany
author_facet Morazán Nuñez, Henris Jobany
Seradj, Ahmad Reza
Álvarez Rodríguez, Javier
Abecia, Leticia
Babot Gaspa, Daniel
Yañez-Ruiz, David R.
Balcells Terés, Joaquim
author_role author
author2 Seradj, Ahmad Reza
Álvarez Rodríguez, Javier
Abecia, Leticia
Babot Gaspa, Daniel
Yañez-Ruiz, David R.
Balcells Terés, Joaquim
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Archaea
Biodiversity
Methane
Slurry
topic Archaea
Biodiversity
Methane
Slurry
description Artificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze-dried dairy cattle feces were used (external archaea) as co-inoculum (Co-i) and structural carbohydrates (CHO: apple pulp; sugar beet pulp; and wheat straw) as substrates. Bottles were incubated (39 +/- 1 degrees C for 56 days), and the gas production was measured (mbar) and converted to the volume. A sample of produced gas was taken and analyzed for methane concentration using a gas chromatography. Bottles were opened at days 0, 25, and 56 to determine total bacteria, total, and hydrogenotrophic methanogens archaea (HMA) concentrations using quantitative polymerase chain reaction and population biodiversity using denaturing gradient gel electrophoresis (DGGE). Incubation time reduced the titers of total bacteria and archaea (P<0.01) but did not modify HMA population. Doses of Co-i showed a positive correlation with HMA titers, although interacted with an incubation period (P<0.001); at 5% Co-inoculation, total bacteria decreased significantly (0-25 days) but remained steady until day 56 (P>0.05), whereas at 10% Co inoculation, titers decreased constantly. Most of the archaeal DGGE bands were observed in all samples, suggesting a common microbial population origin but Co-i supply altered the DGGE structure of archaea populations
publishDate 2015
dc.date.none.fl_str_mv 2015
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1002/ep.11952
http://hdl.handle.net/10459.1/67635
http://hdl.handle.net/10459.1/67635
url https://doi.org/10.1002/ep.11952
http://hdl.handle.net/10459.1/67635
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2010-20820
Versió postprint del document publicat a: https://doi.org/10.1002/ep.11952
Environmental Progress & Sustainable Energy, 2015, vol. 34, num. 1, p. 54-64
dc.rights.none.fl_str_mv (c) American Institute of Chemical Engineers, 2015
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Institute of Chemical Engineers, 2015
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Institute of Chemical Engineers (AIChE)
publisher.none.fl_str_mv American Institute of Chemical Engineers (AIChE)
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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