Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription

[EN]In nature, cells and in particular unicellular microorganisms are exposed to a variety of nutritional environments. Fission yeast cells cultured in nitrogen-rich media grow fast, divide with a large size and show a short G1 and a long G2. However, when cultured in nitrogenpoor media, they exhibi...

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Detalhes bibliográficos
Autores: Rubio, Angela, García-Blanco, Natalia, Vázquez-Bolado, Alicia, Belén Suárez, María, Moreno, Sergio
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/161732
Acesso em linha:http://hdl.handle.net/10366/161732
Access Level:acceso abierto
Palavra-chave:Cell growth
Cell cycle
Nitrogen
Rum1
Ste9
MBF
Schizosaccharomyces pombe
24 Ciencias de la Vida
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spelling Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcriptionRubio, AngelaGarcía-Blanco, NataliaVázquez-Bolado, AliciaBelén Suárez, MaríaMoreno, SergioCell growthCell cycleNitrogenRum1Ste9MBFSchizosaccharomyces pombe24 Ciencias de la Vida[EN]In nature, cells and in particular unicellular microorganisms are exposed to a variety of nutritional environments. Fission yeast cells cultured in nitrogen-rich media grow fast, divide with a large size and show a short G1 and a long G2. However, when cultured in nitrogenpoor media, they exhibit reduced growth rate and cell size and a long G1 and a short G2. In this study, we compared the phenotypes of cells lacking the highly conserved cyclin-dependent kinase (Cdk) inhibitor Rum1 and the anaphase promoting complex/cyclosome (APC/C) activator Ste9 in nitrogen-rich and nitrogen-poor media. Rum1 and Ste9 are dispensable for cell division in nitrogen-rich medium. However, in nitrogen-poor medium they are essential for generating a proper wave of MluI cell-cycle box binding factor (MBF)-dependent transcription at the end of G1, which is crucial for promoting a successful S phase. Mutants lacking Rum1 and Ste9 showed premature entry into S phase and a reduced wave of MBFdependent transcription, leading to replication stress, DNA damage and G2 cell cycle arrest. This work demonstrates how reprogramming the cell cycle by changing the nutritional environment may reveal new roles for cell cycle regulators.Ministerio de Economı́a y Competitividad ; Consejería de Educación y Cultura de Castilla y LeónThe Company of Biologists202520252018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/161732reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésBFU2011-28274BFU2014-55439-RBFU2017-88335-RCSI084U16Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1617322026-06-07T06:28:51Z
dc.title.none.fl_str_mv Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
title Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
spellingShingle Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
Rubio, Angela
Cell growth
Cell cycle
Nitrogen
Rum1
Ste9
MBF
Schizosaccharomyces pombe
24 Ciencias de la Vida
title_short Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
title_full Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
title_fullStr Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
title_full_unstemmed Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
title_sort Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription
dc.creator.none.fl_str_mv Rubio, Angela
García-Blanco, Natalia
Vázquez-Bolado, Alicia
Belén Suárez, María
Moreno, Sergio
author Rubio, Angela
author_facet Rubio, Angela
García-Blanco, Natalia
Vázquez-Bolado, Alicia
Belén Suárez, María
Moreno, Sergio
author_role author
author2 García-Blanco, Natalia
Vázquez-Bolado, Alicia
Belén Suárez, María
Moreno, Sergio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cell growth
Cell cycle
Nitrogen
Rum1
Ste9
MBF
Schizosaccharomyces pombe
24 Ciencias de la Vida
topic Cell growth
Cell cycle
Nitrogen
Rum1
Ste9
MBF
Schizosaccharomyces pombe
24 Ciencias de la Vida
description [EN]In nature, cells and in particular unicellular microorganisms are exposed to a variety of nutritional environments. Fission yeast cells cultured in nitrogen-rich media grow fast, divide with a large size and show a short G1 and a long G2. However, when cultured in nitrogenpoor media, they exhibit reduced growth rate and cell size and a long G1 and a short G2. In this study, we compared the phenotypes of cells lacking the highly conserved cyclin-dependent kinase (Cdk) inhibitor Rum1 and the anaphase promoting complex/cyclosome (APC/C) activator Ste9 in nitrogen-rich and nitrogen-poor media. Rum1 and Ste9 are dispensable for cell division in nitrogen-rich medium. However, in nitrogen-poor medium they are essential for generating a proper wave of MluI cell-cycle box binding factor (MBF)-dependent transcription at the end of G1, which is crucial for promoting a successful S phase. Mutants lacking Rum1 and Ste9 showed premature entry into S phase and a reduced wave of MBFdependent transcription, leading to replication stress, DNA damage and G2 cell cycle arrest. This work demonstrates how reprogramming the cell cycle by changing the nutritional environment may reveal new roles for cell cycle regulators.
publishDate 2018
dc.date.none.fl_str_mv 2018
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/161732
url http://hdl.handle.net/10366/161732
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BFU2011-28274
BFU2014-55439-R
BFU2017-88335-R
CSI084U16
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv The Company of Biologists
publisher.none.fl_str_mv The Company of Biologists
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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