Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae

Ochratoxin A (OTA) is a nephrotoxic mycotoxin which may contaminate various foods and feed products worldwide. Aspergillus niger is one of the species responsible for OTA contamination in grapes and derived products. This species has recently been split into A. niger and Aspergillus welwitschiae. Bo...

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Autores: Abarca Salat, Maria Lourdes|||0000-0002-4151-0758, Bragulat, M. Rosa|||0000-0003-1853-1777, Castellá, Gemma|||0000-0002-8404-0688, Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:199377
Acceso en línea:https://ddd.uab.cat/record/199377
https://dx.doi.org/urn:doi:10.1016/j.ijfoodmicro.2018.11.001
Access Level:acceso abierto
Palabra clave:Microbiologia
Aspergillus niger
Aspergillus welwitschiae
Ecophysiology
Grapes
Ochratoxin A
Raisins
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spelling Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiaeAbarca Salat, Maria Lourdes|||0000-0002-4151-0758Bragulat, M. Rosa|||0000-0003-1853-1777Castellá, Gemma|||0000-0002-8404-0688Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778MicrobiologiaAspergillus nigerAspergillus welwitschiaeEcophysiologyGrapesOchratoxin ARaisinsOchratoxin A (OTA) is a nephrotoxic mycotoxin which may contaminate various foods and feed products worldwide. Aspergillus niger is one of the species responsible for OTA contamination in grapes and derived products. This species has recently been split into A. niger and Aspergillus welwitschiae. Both species can not be distinguished by phenotypic or extrolite profiles and to date there is no ecophysiological information of A. welwitschiae. The aim of this study was to determine the effects of water activity (aw) (0.90; 0.95 and 0.98-0.99), culture media (Yeast Extract Sucrose Broth (YESB); Synthetic Grape Juice Medium (SGM); White grape juice (WGJ)) and temperature (15 °C, 25 °C and 35 °C) on the growth and OTA production of four strains of A. niger and six strains of A. welwitschiae. The assay was performed in microtiter plates, determining the absorbance at 530 nm and the concentration of OTA at 1, 2, 4 and 10 days. No significant differences were observed in absorbance and OTA values between the two species under study. The highest absorbance values were recorded in YESB, followed by SGM and WGJ. Absorbance values increased with increasing aw and temperature. The highest OTA values were obtained at 0.98-0.99 aw and the best culture media for OTA production was YESB, followed by WGJ and SGM. The studied strains of A. niger produced the highest mean OTA level at 25 °C whereas A. welwitschiae strains produced the highest mean OTA concentration at 15 °C, although not differing significantly from concentration produced at 25 °C. To our knowledge, this is the first report on the impact of some environmental factors on growth and OTA production by A. welwitschiae. 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/199377https://dx.doi.org/urn:doi:10.1016/j.ijfoodmicro.2018.11.001reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2014-52516-Ropen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1993772026-06-06T12:50:31Z
dc.title.none.fl_str_mv Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
title Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
spellingShingle Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
Abarca Salat, Maria Lourdes|||0000-0002-4151-0758
Microbiologia
Aspergillus niger
Aspergillus welwitschiae
Ecophysiology
Grapes
Ochratoxin A
Raisins
title_short Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
title_full Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
title_fullStr Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
title_full_unstemmed Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
title_sort Impact of some environmental factors on growth and ochratoxin A production by Aspergillus niger and Aspergillus welwitschiae
dc.creator.none.fl_str_mv Abarca Salat, Maria Lourdes|||0000-0002-4151-0758
Bragulat, M. Rosa|||0000-0003-1853-1777
Castellá, Gemma|||0000-0002-8404-0688
Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
author Abarca Salat, Maria Lourdes|||0000-0002-4151-0758
author_facet Abarca Salat, Maria Lourdes|||0000-0002-4151-0758
Bragulat, M. Rosa|||0000-0003-1853-1777
Castellá, Gemma|||0000-0002-8404-0688
Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
author_role author
author2 Bragulat, M. Rosa|||0000-0003-1853-1777
Castellá, Gemma|||0000-0002-8404-0688
Cabañes Sáenz, Francisco Javier|||0000-0002-2244-5778
author2_role author
author
author
dc.subject.none.fl_str_mv Microbiologia
Aspergillus niger
Aspergillus welwitschiae
Ecophysiology
Grapes
Ochratoxin A
Raisins
topic Microbiologia
Aspergillus niger
Aspergillus welwitschiae
Ecophysiology
Grapes
Ochratoxin A
Raisins
description Ochratoxin A (OTA) is a nephrotoxic mycotoxin which may contaminate various foods and feed products worldwide. Aspergillus niger is one of the species responsible for OTA contamination in grapes and derived products. This species has recently been split into A. niger and Aspergillus welwitschiae. Both species can not be distinguished by phenotypic or extrolite profiles and to date there is no ecophysiological information of A. welwitschiae. The aim of this study was to determine the effects of water activity (aw) (0.90; 0.95 and 0.98-0.99), culture media (Yeast Extract Sucrose Broth (YESB); Synthetic Grape Juice Medium (SGM); White grape juice (WGJ)) and temperature (15 °C, 25 °C and 35 °C) on the growth and OTA production of four strains of A. niger and six strains of A. welwitschiae. The assay was performed in microtiter plates, determining the absorbance at 530 nm and the concentration of OTA at 1, 2, 4 and 10 days. No significant differences were observed in absorbance and OTA values between the two species under study. The highest absorbance values were recorded in YESB, followed by SGM and WGJ. Absorbance values increased with increasing aw and temperature. The highest OTA values were obtained at 0.98-0.99 aw and the best culture media for OTA production was YESB, followed by WGJ and SGM. The studied strains of A. niger produced the highest mean OTA level at 25 °C whereas A. welwitschiae strains produced the highest mean OTA concentration at 15 °C, although not differing significantly from concentration produced at 25 °C. To our knowledge, this is the first report on the impact of some environmental factors on growth and OTA production by A. welwitschiae.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/199377
https://dx.doi.org/urn:doi:10.1016/j.ijfoodmicro.2018.11.001
url https://ddd.uab.cat/record/199377
https://dx.doi.org/urn:doi:10.1016/j.ijfoodmicro.2018.11.001
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2014-52516-R
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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