Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways

Direct visualization of pathways followed by single molecules while they spontaneously self-assemble into supramolecular biological machines may provide fundamental knowledge to guide molecular therapeutics and the bottom-up design of nanomaterials and nanodevices. Here, high-speed atomic force micr...

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Detalles Bibliográficos
Autores: Valbuena, Alejandro, Maity, Sourav, Mateu, Mauricio G., Roos, Wouter H.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/241852
Acceso en línea:http://hdl.handle.net/10261/241852
Access Level:acceso abierto
Palabra clave:Self-assembly
Nanomaterials
Virus
Capsid proteins
Single-molecule
High-speed atomic force microscopy
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spelling Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic PathwaysValbuena, AlejandroMaity, SouravMateu, Mauricio G.Roos, Wouter H.Self-assemblyNanomaterialsVirusCapsid proteinsSingle-moleculeHigh-speed atomic force microscopyDirect visualization of pathways followed by single molecules while they spontaneously self-assemble into supramolecular biological machines may provide fundamental knowledge to guide molecular therapeutics and the bottom-up design of nanomaterials and nanodevices. Here, high-speed atomic force microscopy is used to visualize self-assembly of the bidimensional lattice of protein molecules that constitutes the framework of the mature human immunodeficiency virus capsid. By real-time imaging of the assembly reaction, individual transient intermediates and reaction pathways followed by single molecules could be revealed. As when assembling a jigsaw puzzle, the capsid protein lattice is randomly built. Lattice patches grow independently from separate nucleation events whereby individual molecules follow different paths. Protein subunits can be added individually, while others form oligomers before joining a lattice or are occasionally removed from the latter. Direct real-time imaging of supramolecular self-assembly has revealed a complex, chaotic process involving multiple routes followed by individual molecules that are inaccessible to bulk (averaging) techniques.MICINN/FEDER EU (Spain, BIO2015-69928-R and RTI2018-096635-B-100) to M.G.M. and a VIDI grant from the Nederlandse Organisatie voor Wetenschappelijk Onderzoek and a STW-Perspectief grant “Cancer-ID” to W.H.R. A travel grant to A.V. by the European ARBRE-Mobieu ConsortiumAmerican Chemical SocietyMinisterio de Ciencia e Innovación (España)Netherlands Organization for Scientific ResearchConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/241852reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1021/acsnano.0c03207Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2418522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
title Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
spellingShingle Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
Valbuena, Alejandro
Self-assembly
Nanomaterials
Virus
Capsid proteins
Single-molecule
High-speed atomic force microscopy
title_short Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
title_full Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
title_fullStr Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
title_full_unstemmed Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
title_sort Visualization of Single Molecules Building a Viral Capsid Protein Lattice through Stochastic Pathways
dc.creator.none.fl_str_mv Valbuena, Alejandro
Maity, Sourav
Mateu, Mauricio G.
Roos, Wouter H.
author Valbuena, Alejandro
author_facet Valbuena, Alejandro
Maity, Sourav
Mateu, Mauricio G.
Roos, Wouter H.
author_role author
author2 Maity, Sourav
Mateu, Mauricio G.
Roos, Wouter H.
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Netherlands Organization for Scientific Research
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Self-assembly
Nanomaterials
Virus
Capsid proteins
Single-molecule
High-speed atomic force microscopy
topic Self-assembly
Nanomaterials
Virus
Capsid proteins
Single-molecule
High-speed atomic force microscopy
description Direct visualization of pathways followed by single molecules while they spontaneously self-assemble into supramolecular biological machines may provide fundamental knowledge to guide molecular therapeutics and the bottom-up design of nanomaterials and nanodevices. Here, high-speed atomic force microscopy is used to visualize self-assembly of the bidimensional lattice of protein molecules that constitutes the framework of the mature human immunodeficiency virus capsid. By real-time imaging of the assembly reaction, individual transient intermediates and reaction pathways followed by single molecules could be revealed. As when assembling a jigsaw puzzle, the capsid protein lattice is randomly built. Lattice patches grow independently from separate nucleation events whereby individual molecules follow different paths. Protein subunits can be added individually, while others form oligomers before joining a lattice or are occasionally removed from the latter. Direct real-time imaging of supramolecular self-assembly has revealed a complex, chaotic process involving multiple routes followed by individual molecules that are inaccessible to bulk (averaging) techniques.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/241852
url http://hdl.handle.net/10261/241852
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1021/acsnano.0c03207
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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