Immune dysregulation, apoptosis impairment, and enhanced seroreactivity to Anisakis simplex in Crohn's disease: interplay of IL-7/IL-7R signalling and CD132 deficiency
BACKGROUND: In previous studies, we identified a deficiency of gammadelta T cells and an increased prevalence of anti-Anisakis simplex antibodies in patients with Crohn's disease (CD). Additionally, decreased gene expression of the interleukin 2 (IL-2) receptor gamma subunit (CD132) was observe...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO) |
| Repositorio: | r-FISABIO. Repositorio Institucional de Producción Científica |
| OAI Identifier: | oai:dnet:r-fisabio___::82699f240567edbf784c26e1d0c4b352 |
| Acceso en línea: | https://fisabio.portalinvestigacion.com/publicaciones/21116 |
| Access Level: | acceso abierto |
| Palabra clave: | Crohn's disease anti-Anisakis simplex antibodies IL-7 gene expression IL-2 receptor subunit gamma (CD 132) caspase-3 |
| Sumario: | BACKGROUND: In previous studies, we identified a deficiency of gammadelta T cells and an increased prevalence of anti-Anisakis simplex antibodies in patients with Crohn's disease (CD). Additionally, decreased gene expression of the interleukin 2 (IL-2) receptor gamma subunit (CD132) was observed in tissues from CD patients. OBJECTIVE: To analyse the gene expression of IL-7 and its receptors in tissues from CD patients and to explore its relationship with anti-A. simplex antibodies. METHODS: 52 patients diagnosed with CD were compared with a control group of 52 healthy individuals. Peripheral blood samples were analysed to assess levels of anti-A. simplex antibodies and IL-7. In addition, intestinal tissue samples from 20 subjects in each group were examined to evaluate IL-7 gene expression, IL-7 protein levels, the IL-2 receptor gamma subunit (CD132), the IL-7 receptor alpha subunit (CD127), and caspase-3 expression. FINDINGS: Anti-A. simplex antibody levels were elevated in patients with CD. Caspase-3 expression was significantly reduced in the tissues of CD patients with anti-A. simplex IgA, and this reduction extended to IgG and IgE in healthy individuals. A negative correlation was observed between caspase-3 levels and serum anti-A. simplex IgA, as well as IL-7 levels in the tissues of CD patients. In healthy subjects, tissue IL-7 levels were lower in those positive for anti-A. simplex IgA, while serum IL-7 levels were higher in individuals positive for anti-A. simplex IgG. MAIN CONCLUSIONS: This study revealed the interplay between IL-7 signalling, gammadelta T cell deficiency, and immune responses to A. simplex in CD. Our findings underscored a cause-effect relationship between CD132 deficiency, gammadelta T cell depletion, and defective mucosal immunity, which may drive both CD inflammation and susceptibility to parasitic infections like A. simplex. |
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