Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke

At present, many studies support the notion that after stroke, remote regions connected to the infarcted area are also affected and may contribute to functional outcome. In the present study, we have analyzed possible microanatomical alterations in pyramidal neurons from the contralesional hemispher...

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Autores: Merino Serrais, Paula, Plaza Alonso, Sergio, Hellal, Farida, Valero Freitag, Susana, Kastanauskaite, Asta, Muñoz Céspedes, Alberto, Plesnila, Nikolaus, Felipe, Javier de
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/72133
Acceso en línea:https://hdl.handle.net/20.500.14352/72133
Access Level:acceso abierto
Palabra clave:576
612.82
cerebral cortex
dendritic spines
intracellular injections
neuronal complexity
tMCAo
Neurociencias (Medicina)
Biología celular (Biología)
2490 Neurociencias
2407 Biología Celular
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spelling Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic strokeMerino Serrais, PaulaPlaza Alonso, SergioHellal, FaridaValero Freitag, SusanaKastanauskaite, AstaMuñoz Céspedes, AlbertoPlesnila, NikolausFelipe, Javier de576612.82cerebral cortexdendritic spinesintracellular injectionsneuronal complexitytMCAoNeurociencias (Medicina)Biología celular (Biología)2490 Neurociencias2407 Biología CelularAt present, many studies support the notion that after stroke, remote regions connected to the infarcted area are also affected and may contribute to functional outcome. In the present study, we have analyzed possible microanatomical alterations in pyramidal neurons from the contralesional hemisphere after induced stroke. We performed intracellular injections of Lucifer yellow in pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere in an ischemic stroke mouse model. A detailed 3-dimensional analysis of the neuronal complexity and morphological alterations of dendritic spines was then performed. Our results demonstrate that pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere show selective changes in their dendritic arbors, namely, less dendritic complexity of the apical dendritic arbor—but no changes in the basal dendritic arbor. In addition, we found differences in spine morphology in both apical and basal dendrites comparing the contralesional hemisphere with the lesional hemisphere. Our results show that pyramidal neurons of remote areas connected to the infarct zone exhibit a series of selective changes in neuronal complexity and morphological distribution of dendritic spines, supporting the hypothesis that remote regions connected to the peri-infarcted area are also affected after stroke.Oxford OpenUniversidad Complutense de Madrid20222022-03-3020222022-03-30journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/72133reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial 3.0 Españahttps://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/721332026-06-02T12:44:21Z
dc.title.none.fl_str_mv Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
title Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
spellingShingle Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
Merino Serrais, Paula
576
612.82
cerebral cortex
dendritic spines
intracellular injections
neuronal complexity
tMCAo
Neurociencias (Medicina)
Biología celular (Biología)
2490 Neurociencias
2407 Biología Celular
title_short Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
title_full Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
title_fullStr Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
title_full_unstemmed Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
title_sort Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
dc.creator.none.fl_str_mv Merino Serrais, Paula
Plaza Alonso, Sergio
Hellal, Farida
Valero Freitag, Susana
Kastanauskaite, Asta
Muñoz Céspedes, Alberto
Plesnila, Nikolaus
Felipe, Javier de
author Merino Serrais, Paula
author_facet Merino Serrais, Paula
Plaza Alonso, Sergio
Hellal, Farida
Valero Freitag, Susana
Kastanauskaite, Asta
Muñoz Céspedes, Alberto
Plesnila, Nikolaus
Felipe, Javier de
author_role author
author2 Plaza Alonso, Sergio
Hellal, Farida
Valero Freitag, Susana
Kastanauskaite, Asta
Muñoz Céspedes, Alberto
Plesnila, Nikolaus
Felipe, Javier de
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 576
612.82
cerebral cortex
dendritic spines
intracellular injections
neuronal complexity
tMCAo
Neurociencias (Medicina)
Biología celular (Biología)
2490 Neurociencias
2407 Biología Celular
topic 576
612.82
cerebral cortex
dendritic spines
intracellular injections
neuronal complexity
tMCAo
Neurociencias (Medicina)
Biología celular (Biología)
2490 Neurociencias
2407 Biología Celular
description At present, many studies support the notion that after stroke, remote regions connected to the infarcted area are also affected and may contribute to functional outcome. In the present study, we have analyzed possible microanatomical alterations in pyramidal neurons from the contralesional hemisphere after induced stroke. We performed intracellular injections of Lucifer yellow in pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere in an ischemic stroke mouse model. A detailed 3-dimensional analysis of the neuronal complexity and morphological alterations of dendritic spines was then performed. Our results demonstrate that pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere show selective changes in their dendritic arbors, namely, less dendritic complexity of the apical dendritic arbor—but no changes in the basal dendritic arbor. In addition, we found differences in spine morphology in both apical and basal dendrites comparing the contralesional hemisphere with the lesional hemisphere. Our results show that pyramidal neurons of remote areas connected to the infarct zone exhibit a series of selective changes in neuronal complexity and morphological distribution of dendritic spines, supporting the hypothesis that remote regions connected to the peri-infarcted area are also affected after stroke.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-03-30
2022
2022-03-30
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/72133
url https://hdl.handle.net/20.500.14352/72133
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial 3.0 España
https://creativecommons.org/licenses/by-nc/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial 3.0 España
https://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Open
publisher.none.fl_str_mv Oxford Open
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.198674

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