The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor

In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)-dependent transcriptio...

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Authors: Ivanova, Tsvetomira Georgieva, 1978-, Alves-Rodrigues, Isabel, Gómez Escoda, Blanca, Dutta, Chaitali, DeCaprio, James A., Rhind, Nick, Hidalgo Hernando, Elena, Ayté del Olmo, José
Format: article
Status:Published version
Publication Date:2013
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/25284
Online Access:http://hdl.handle.net/10230/25284
http://dx.doi.org/10.1091/mbc.E13-05-0257
Access Level:Open access
Keyword:ADN
Proteïnes quinases -- Metabolisme
Schizosaccharomyces pombe -- Metabolisme
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spelling The DNA damage and the DNA replication checkpoints converge at the MBF transcription factorIvanova, Tsvetomira Georgieva, 1978-Alves-Rodrigues, IsabelGómez Escoda, BlancaDutta, ChaitaliDeCaprio, James A.Rhind, NickHidalgo Hernando, ElenaAyté del Olmo, JoséADNProteïnes quinases -- MetabolismeSchizosaccharomyces pombe -- MetabolismeIn fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)-dependent transcription. This is essential to reinitiate DNA synthesis and for correct G1-to-S transition. Here we show that Cdc10, which is an essential part of the MBF core, is the target of the DNA damage checkpoint. When fission yeast cells are treated with DNA-damaging agents, Chk1 is activated and phosphorylates Cdc10 at its carboxy-terminal domain. This modification is responsible for the repression of MBF-dependent transcription through induced release of MBF from chromatin. This inactivation of MBF is important for survival of cells challenged with DNA-damaging agents. Thus Yox1 and Cdc10 couple normal cell cycle regulation in unperturbed conditions and the DNA replication and DNA damage checkpoints into a single transcriptional complex.This work was supported by grants from the Spanish Ministry of Science and Innovation (BFU2009–07453 and BFU2012-31939), PLAN E and FEDER, Consolider-Ingenio 2007–0020, and SGR2009-195 from the Generalitat de Catalunya. J.A. and E.H. are recipients of ICREA Academia Awards (Generalitat de Catalunya)American Society for Cell Biology201520152013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25284http://dx.doi.org/10.1091/mbc.E13-05-0257reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésMolecular biology of the cell. 2013;24(21):3350-7info:eu-repo/grantAgreement/ES/3PN/BFU2009-07453info:eu-repo/grantAgreement/ES/3PN/BFU2012-31939© 2013 Ivanova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0)./n“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.http://creativecommons.org/licenses/by-nc-sa/3.0info:eu-repo/semantics/openAccessoai:recercat.cat:10230/252842026-05-29T05:05:01Z
dc.title.none.fl_str_mv The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
spellingShingle The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
Ivanova, Tsvetomira Georgieva, 1978-
ADN
Proteïnes quinases -- Metabolisme
Schizosaccharomyces pombe -- Metabolisme
title_short The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_full The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_fullStr The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_full_unstemmed The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_sort The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
dc.creator.none.fl_str_mv Ivanova, Tsvetomira Georgieva, 1978-
Alves-Rodrigues, Isabel
Gómez Escoda, Blanca
Dutta, Chaitali
DeCaprio, James A.
Rhind, Nick
Hidalgo Hernando, Elena
Ayté del Olmo, José
author Ivanova, Tsvetomira Georgieva, 1978-
author_facet Ivanova, Tsvetomira Georgieva, 1978-
Alves-Rodrigues, Isabel
Gómez Escoda, Blanca
Dutta, Chaitali
DeCaprio, James A.
Rhind, Nick
Hidalgo Hernando, Elena
Ayté del Olmo, José
author_role author
author2 Alves-Rodrigues, Isabel
Gómez Escoda, Blanca
Dutta, Chaitali
DeCaprio, James A.
Rhind, Nick
Hidalgo Hernando, Elena
Ayté del Olmo, José
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADN
Proteïnes quinases -- Metabolisme
Schizosaccharomyces pombe -- Metabolisme
topic ADN
Proteïnes quinases -- Metabolisme
Schizosaccharomyces pombe -- Metabolisme
description In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)-dependent transcription. This is essential to reinitiate DNA synthesis and for correct G1-to-S transition. Here we show that Cdc10, which is an essential part of the MBF core, is the target of the DNA damage checkpoint. When fission yeast cells are treated with DNA-damaging agents, Chk1 is activated and phosphorylates Cdc10 at its carboxy-terminal domain. This modification is responsible for the repression of MBF-dependent transcription through induced release of MBF from chromatin. This inactivation of MBF is important for survival of cells challenged with DNA-damaging agents. Thus Yox1 and Cdc10 couple normal cell cycle regulation in unperturbed conditions and the DNA replication and DNA damage checkpoints into a single transcriptional complex.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25284
http://dx.doi.org/10.1091/mbc.E13-05-0257
url http://hdl.handle.net/10230/25284
http://dx.doi.org/10.1091/mbc.E13-05-0257
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular biology of the cell. 2013;24(21):3350-7
info:eu-repo/grantAgreement/ES/3PN/BFU2009-07453
info:eu-repo/grantAgreement/ES/3PN/BFU2012-31939
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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