Increased Levels of Diadenosine Polyphosphates in Dry Eye

Purpose. To analyze the levels of the diadenosine polyphosphates Ap4A and Ap5A in tears, in a set of control subjects and in groups of symptomatic and nonsymptomatic persons with dry eye. Methods. Ninety-seven subjects participated in the study. The subjects were divided into five experimental group...

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Detalles Bibliográficos
Autores: Peral Cerda, María Asunción, Carracedo Rodríguez, Juan Gonzalo, Acosta Boj, María Carmen, Gallar Martínez, Juana, Pintor Just, Jesús Jerónimo
Tipo de recurso: artículo
Fecha de publicación:2006
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/51961
Acceso en línea:https://hdl.handle.net/20.500.14352/51961
Access Level:acceso abierto
Palabra clave:577.15
617.764
Bodily secretions
Diadenosine poliphosphates
Dry eye
Bioquímica (Química)
Oftalmología
Anatomía ocular
3201.09 Oftalmología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/51961
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network_name_str España
repository_id_str
spelling Increased Levels of Diadenosine Polyphosphates in Dry EyePeral Cerda, María AsunciónCarracedo Rodríguez, Juan GonzaloAcosta Boj, María CarmenGallar Martínez, JuanaPintor Just, Jesús Jerónimo577.15617.764Bodily secretionsDiadenosine poliphosphatesDry eyeBioquímica (Química)OftalmologíaAnatomía ocular3201.09 OftalmologíaPurpose. To analyze the levels of the diadenosine polyphosphates Ap4A and Ap5A in tears, in a set of control subjects and in groups of symptomatic and nonsymptomatic persons with dry eye. Methods. Ninety-seven subjects participated in the study. The subjects were divided into five experimental groups: control subjects; symptomatic patients with normal tear secretion; symptomatic patients with low tear secretion; forced blink; and corneal mechanical stimulation provided by a gas esthesiometer. The Schirmer I test was used to measure and collect tear secretions from each subject. All samples were processed by high pressure liquid chromatography (HPLC) and their Ap4A and Ap5A levels determined. Results. The levels of Ap4A and Ap5A in tears were greater in all symptomatic patients than in control subjects, especially in symptomatic subjects with low tear secretion. Within the symptomatic subjects with normal tear secretion, significant differences in concentrations of Ap4A and Ap5A were found between men and women. In the forced blink experiments, concentrations of the Ap4A and Ap5A rose with increasing blink frequency. When the cornea was mechanically stimulated, the levels of Ap4A and Ap5A rose significantly during both moderate and high-flow rate tests. Conclusions. The increased levels of Ap4A and Ap5A in tears of patients with dry eye allow these dinucleotides to be used as objective biomarkers in dry eye conditions.The Association for Research in Vision and Ophthalmology, Inc.Universidad Complutense de Madrid20062006-09-0120062006-09-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/51961reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/519612026-06-02T12:44:21Z
dc.title.none.fl_str_mv Increased Levels of Diadenosine Polyphosphates in Dry Eye
title Increased Levels of Diadenosine Polyphosphates in Dry Eye
spellingShingle Increased Levels of Diadenosine Polyphosphates in Dry Eye
Peral Cerda, María Asunción
577.15
617.764
Bodily secretions
Diadenosine poliphosphates
Dry eye
Bioquímica (Química)
Oftalmología
Anatomía ocular
3201.09 Oftalmología
title_short Increased Levels of Diadenosine Polyphosphates in Dry Eye
title_full Increased Levels of Diadenosine Polyphosphates in Dry Eye
title_fullStr Increased Levels of Diadenosine Polyphosphates in Dry Eye
title_full_unstemmed Increased Levels of Diadenosine Polyphosphates in Dry Eye
title_sort Increased Levels of Diadenosine Polyphosphates in Dry Eye
dc.creator.none.fl_str_mv Peral Cerda, María Asunción
Carracedo Rodríguez, Juan Gonzalo
Acosta Boj, María Carmen
Gallar Martínez, Juana
Pintor Just, Jesús Jerónimo
author Peral Cerda, María Asunción
author_facet Peral Cerda, María Asunción
Carracedo Rodríguez, Juan Gonzalo
Acosta Boj, María Carmen
Gallar Martínez, Juana
Pintor Just, Jesús Jerónimo
author_role author
author2 Carracedo Rodríguez, Juan Gonzalo
Acosta Boj, María Carmen
Gallar Martínez, Juana
Pintor Just, Jesús Jerónimo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.15
617.764
Bodily secretions
Diadenosine poliphosphates
Dry eye
Bioquímica (Química)
Oftalmología
Anatomía ocular
3201.09 Oftalmología
topic 577.15
617.764
Bodily secretions
Diadenosine poliphosphates
Dry eye
Bioquímica (Química)
Oftalmología
Anatomía ocular
3201.09 Oftalmología
description Purpose. To analyze the levels of the diadenosine polyphosphates Ap4A and Ap5A in tears, in a set of control subjects and in groups of symptomatic and nonsymptomatic persons with dry eye. Methods. Ninety-seven subjects participated in the study. The subjects were divided into five experimental groups: control subjects; symptomatic patients with normal tear secretion; symptomatic patients with low tear secretion; forced blink; and corneal mechanical stimulation provided by a gas esthesiometer. The Schirmer I test was used to measure and collect tear secretions from each subject. All samples were processed by high pressure liquid chromatography (HPLC) and their Ap4A and Ap5A levels determined. Results. The levels of Ap4A and Ap5A in tears were greater in all symptomatic patients than in control subjects, especially in symptomatic subjects with low tear secretion. Within the symptomatic subjects with normal tear secretion, significant differences in concentrations of Ap4A and Ap5A were found between men and women. In the forced blink experiments, concentrations of the Ap4A and Ap5A rose with increasing blink frequency. When the cornea was mechanically stimulated, the levels of Ap4A and Ap5A rose significantly during both moderate and high-flow rate tests. Conclusions. The increased levels of Ap4A and Ap5A in tears of patients with dry eye allow these dinucleotides to be used as objective biomarkers in dry eye conditions.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-09-01
2006
2006-09-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/51961
url https://hdl.handle.net/20.500.14352/51961
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Association for Research in Vision and Ophthalmology, Inc.
publisher.none.fl_str_mv The Association for Research in Vision and Ophthalmology, Inc.
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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