Monogalactosyldiacylglycerol and digalactosyldiacylglycerol role, physical states, applications and biomimetic monolayer films

The relevance of biomimetic membranes using galactolipids has not been expressed in any extensive experimental study of these lipids. Thus, on the one hand, we present an in-depth article about the presence and role of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) in thyl...

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Detalles Bibliográficos
Autores: Hoyo Pérez, Javier|||0000-0002-9927-2465, Guaus Guerrero, Ester|||0000-0001-9712-5325, Torrent Burgués, Juan|||0000-0002-4952-736X
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/89815
Acceso en línea:https://hdl.handle.net/2117/89815
https://dx.doi.org/10.1140/epje/i2016-16039-0
Access Level:acceso abierto
Palabra clave:Membranes (Biology)
Supported lipid-bilayers
dna-polymerase-activity
air-water-interface
photosystem-ii
dipalmitoylphosphatidylcholine monolayers
glycolipids fraction
force microscopy
membranes
spinach
ito
Membranes (Biologia)
Àrees temàtiques de la UPC::Enginyeria química
Descripción
Sumario:The relevance of biomimetic membranes using galactolipids has not been expressed in any extensive experimental study of these lipids. Thus, on the one hand, we present an in-depth article about the presence and role of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) in thylakoid membranes, their physical states and their applications. On the other hand, we use the Langmuir and Langmuir-Blodgett (LB) techniques to prepare biomimetic monolayers of saturated galactolipids MGDG, DGDG and MGDG:DGDG 2:1 mixture (MD)-biological ratio-. These monolayers are studied using surface pressure-area isotherms and their data are processed to enlighten their physical states and mixing behaviour. These monolayers, once transferred to a solid substrate at several surface pressures are topographically studied on mica using atomic force microscopy (AFM) and using cyclic voltammetry for studying the electrochemical behaviour of the monolayers once transferred to indium-tin oxide (ITO), which has good optical and electrical properties. Moreover, MD presents other differences in comparison with its pure components that are explained by the presence of different kinds of galactosyl headgroups that restrict the optimal orientation of the MGDG headgroups.