2D-NMR demonstration of lignin removal from wood and non-wood plant feedstocks by fungal versatile peroxidase
The ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escher...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | otro |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/98704 |
| Acceso en línea: | http://hdl.handle.net/10261/98704 |
| Access Level: | acceso abierto |
| Palabra clave: | Lignin Enzymes Versatile peroxidase Veratryl alcohol 2D NMR Eucalyptus |
| Sumario: | The ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escherichia coli and "in vitro" folded. Two-dimensional nuclear magnetic resonance (NMR) of the treated materials, swollen in dimethylsulfoxide-d6, revealed a reduction in the number of lignin side-chain linkages after both treatments. However, a significant removal of lignin by the enzymatic treatment was only observed in the case of eucalypt wood, as shown by the decrease of lignin aromatic signals referred to carbohydrate anomeric signals. |
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