Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes
Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two p...
| Autores: | , , |
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| Tipo de recurso: | capítulo de libro |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/14029 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/14029 |
| Access Level: | acceso abierto |
| Palabra clave: | 535 Partially coherent-Light Reconstruction Algorithms Óptica (Física) 2209.19 Óptica Física |
| Sumario: | Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed. |
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