Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants

A collection of 163 strains of black yeast-like fungi from the CBS Fungal Biodiversity Center (Utrecht, The Netherlands), has been screened for the ability to grow on hexadecane, toluene and polychlorinated biphenyl 126 (PCB126) as the sole carbon and energy source. These compounds were chosen as re...

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Autores: Blasi, Barbara, Poyntner, Caroline, Rudavsky, Tamara, Prenafeta-Boldú, Francesc X., de Hoog, Sybren, Tafer, Hakim, Sterflinger, Katja
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/1861
Acceso en línea:http://hdl.handle.net/20.500.12327/1861
https://doi.org/10.1080/01490451.2015.1052118
Access Level:acceso abierto
Palabra clave:502
504
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spelling Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of PollutantsBlasi, BarbaraPoyntner, CarolineRudavsky, TamaraPrenafeta-Boldú, Francesc X.de Hoog, SybrenTafer, HakimSterflinger, Katja502504A collection of 163 strains of black yeast-like fungi from the CBS Fungal Biodiversity Center (Utrecht, The Netherlands), has been screened for the ability to grow on hexadecane, toluene and polychlorinated biphenyl 126 (PCB126) as the sole carbon and energy source. These compounds were chosen as representatives of relevant environmental pollutants. A microtiter plate-based culture assay was set up in order to screen the fungal strains for growth on the selected xenobiotics versus glucose, as a positive control. Growth was observed in 25 strains on at least two of the tested substrates. Confirmation of substrate assimilation was performed by cultivation on closed vials and analysis of the headspace composition with regard to the added volatile substrates and the generated carbon dioxide. Exophiala mesophila (CBS 120910) and Cladophialophora immunda (CBS 110551), both of the order Chaetothyriales and isolated from a patient with chronic sinusitis and a polluted soil sample, respectively, showed the ability to grow on toluene as the sole carbon and energy source. Toluene assimilation has previously been described for C. immunda but this is the first account for E. mesophila. Also, this is the first time that the capacity to grow on alkylbenzenes has been demonstrated for a clinical isolate. Assimilation of toluene could not be demonstrated for the human opportunistic pathogen Pseudoallescheria boydii (CBS 115.59, Microascales), but the results from microtiter plate assays suggest that strains of this species are promising candidates for further studies. The outstanding abilities of black yeast-like fungi to thrive in extreme environments makes them ideal agents for the bioremediation of polluted soils, and for the treatment of contaminated gas streams in biofilters. However, interrelations between hydrocarbonoclastic and potentially pathogenic strains need to be elucidated in order to avoid the possibility of biohazards occurring.info:eu-repo/semantics/publishedVersionTaylor and FrancisProducció AnimalSostenibilitat en Biosistemes202220222016info:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/20.500.12327/1861https://doi.org/10.1080/01490451.2015.1052118reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésGeomicrobiology JournalAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/18612026-06-16T08:51:17Z
dc.title.none.fl_str_mv Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
title Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
spellingShingle Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
Blasi, Barbara
502
504
title_short Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
title_full Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
title_fullStr Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
title_full_unstemmed Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
title_sort Pathogenic Yet Environmentally Friendly? Black Fungal Candidates for Bioremediation of Pollutants
dc.creator.none.fl_str_mv Blasi, Barbara
Poyntner, Caroline
Rudavsky, Tamara
Prenafeta-Boldú, Francesc X.
de Hoog, Sybren
Tafer, Hakim
Sterflinger, Katja
author Blasi, Barbara
author_facet Blasi, Barbara
Poyntner, Caroline
Rudavsky, Tamara
Prenafeta-Boldú, Francesc X.
de Hoog, Sybren
Tafer, Hakim
Sterflinger, Katja
author_role author
author2 Poyntner, Caroline
Rudavsky, Tamara
Prenafeta-Boldú, Francesc X.
de Hoog, Sybren
Tafer, Hakim
Sterflinger, Katja
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sostenibilitat en Biosistemes
dc.subject.none.fl_str_mv 502
504
topic 502
504
description A collection of 163 strains of black yeast-like fungi from the CBS Fungal Biodiversity Center (Utrecht, The Netherlands), has been screened for the ability to grow on hexadecane, toluene and polychlorinated biphenyl 126 (PCB126) as the sole carbon and energy source. These compounds were chosen as representatives of relevant environmental pollutants. A microtiter plate-based culture assay was set up in order to screen the fungal strains for growth on the selected xenobiotics versus glucose, as a positive control. Growth was observed in 25 strains on at least two of the tested substrates. Confirmation of substrate assimilation was performed by cultivation on closed vials and analysis of the headspace composition with regard to the added volatile substrates and the generated carbon dioxide. Exophiala mesophila (CBS 120910) and Cladophialophora immunda (CBS 110551), both of the order Chaetothyriales and isolated from a patient with chronic sinusitis and a polluted soil sample, respectively, showed the ability to grow on toluene as the sole carbon and energy source. Toluene assimilation has previously been described for C. immunda but this is the first account for E. mesophila. Also, this is the first time that the capacity to grow on alkylbenzenes has been demonstrated for a clinical isolate. Assimilation of toluene could not be demonstrated for the human opportunistic pathogen Pseudoallescheria boydii (CBS 115.59, Microascales), but the results from microtiter plate assays suggest that strains of this species are promising candidates for further studies. The outstanding abilities of black yeast-like fungi to thrive in extreme environments makes them ideal agents for the bioremediation of polluted soils, and for the treatment of contaminated gas streams in biofilters. However, interrelations between hydrocarbonoclastic and potentially pathogenic strains need to be elucidated in order to avoid the possibility of biohazards occurring.
publishDate 2016
dc.date.none.fl_str_mv 2016
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1861
https://doi.org/10.1080/01490451.2015.1052118
url http://hdl.handle.net/20.500.12327/1861
https://doi.org/10.1080/01490451.2015.1052118
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Geomicrobiology Journal
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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