3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine

The current study reports on the manufacturing by rapid prototyping technique of three-dimensional (3D) scaffolds based on silicon substituted hydroxyapatite with Elastin-like Recombinamers (ELRs) functionalized surfaces. Silicon doped hydroxyapatite (Si-HA), with Ca-10(PO4)(5.7)(SiO4)(0.3)(OH)(1.7)...

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Detalles Bibliográficos
Autores: Vila, Mercedes, García, Ana, Girotti, Alessandra, Alonso, Matilde, Rodriguez Cabello, José Carlos, González Vázquez, Arlyng, Planell, Josep A., Engel, Elisabeth, Buján, Julia, Garcia-Honduvilla, Natalio, Vallet Regí, María Dulce Nombre
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/23100
Acceso en línea:https://hdl.handle.net/20.500.14352/23100
Access Level:acceso abierto
Palabra clave:546
615.46
Bone repair
Tissue engineering
Elastin-like recombinamers (ELRs)
Silicon doped hydroxyapatite (Si-HA)
Rapid prototyped 3D scaffolds
Bone marrow Mesenchymal Stromal Cells (BMSCs)
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/23100
network_acronym_str ES
network_name_str España
repository_id_str
spelling 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicineVila, MercedesGarcía, AnaGirotti, AlessandraAlonso, MatildeRodriguez Cabello, José CarlosGonzález Vázquez, ArlyngPlanell, Josep A.Engel, ElisabethBuján, JuliaGarcia-Honduvilla, NatalioVallet Regí, María Dulce Nombre546615.46Bone repairTissue engineeringElastin-like recombinamers (ELRs)Silicon doped hydroxyapatite (Si-HA)Rapid prototyped 3D scaffoldsBone marrow Mesenchymal Stromal Cells (BMSCs)MaterialesQuímica inorgánica (Química)3312 Tecnología de Materiales2303 Química InorgánicaThe current study reports on the manufacturing by rapid prototyping technique of three-dimensional (3D) scaffolds based on silicon substituted hydroxyapatite with Elastin-like Recombinamers (ELRs) functionalized surfaces. Silicon doped hydroxyapatite (Si-HA), with Ca-10(PO4)(5.7)(SiO4)(0.3)(OH)(1.7)h(0.3) nominal formula, was surface functionalized with two different types of polymers designed by genetic engineering: ELR-RGD that contain cell attachment specific sequences and ELR-SNA15/RGD with both hydroxyapatite and cells domains that interact with the inorganic phase and with the cells, respectively. These hybrid materials were subjected to in vitro assays in order to clarify if the ELRs coating improved the well-known biocompatible and bone regeneration properties of calcium phosphates materials. The in vitro tests showed that there was a total and homogeneous colonization of the 3D scaffolds by Bone marrow Mesenchymal Stromal Cells (BMSCs). In addition, the BMSCs were viable and able to proliferate and differentiate into osteoblasts. Statement of Significance Bone tissue engineering is an area of increasing interest because its main applications are directly related to the rising life expectancy of the population, which promotes higher rates of several bone pathologies, so innovative strategies are needed for bone tissue regeneration therapies. Here we use the rapid prototyping technology to allow moulding ceramic 3D scaffolds and we use different bio-polymers for the functionalization of their surfaces in order to enhance the biological response. Combining the ceramic material (silicon doped hydroxyapatite, Si-HA) and the Elastin like Recombinamers (ELRs) polymers with the presence of the integrin-mediate adhesion domain alone or in combination with SNAI 5 peptide that possess high affinity for hydroxyapatite, provided an improved Bone marrow Mesenchymal Stromal Cells (BMSCs) differentiation into osteoblastic linkage. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.ElsevierUniversidad Complutense de Madrid20162016-01-0120162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/23100reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/231002026-06-02T12:44:21Z
dc.title.none.fl_str_mv 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
title 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
spellingShingle 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
Vila, Mercedes
546
615.46
Bone repair
Tissue engineering
Elastin-like recombinamers (ELRs)
Silicon doped hydroxyapatite (Si-HA)
Rapid prototyped 3D scaffolds
Bone marrow Mesenchymal Stromal Cells (BMSCs)
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
title_short 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
title_full 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
title_fullStr 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
title_full_unstemmed 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
title_sort 3D silicon doped hydroxyapatite scaffolds decorated with Elastin-like Recombinamers for bone regenerative medicine
dc.creator.none.fl_str_mv Vila, Mercedes
García, Ana
Girotti, Alessandra
Alonso, Matilde
Rodriguez Cabello, José Carlos
González Vázquez, Arlyng
Planell, Josep A.
Engel, Elisabeth
Buján, Julia
Garcia-Honduvilla, Natalio
Vallet Regí, María Dulce Nombre
author Vila, Mercedes
author_facet Vila, Mercedes
García, Ana
Girotti, Alessandra
Alonso, Matilde
Rodriguez Cabello, José Carlos
González Vázquez, Arlyng
Planell, Josep A.
Engel, Elisabeth
Buján, Julia
Garcia-Honduvilla, Natalio
Vallet Regí, María Dulce Nombre
author_role author
author2 García, Ana
Girotti, Alessandra
Alonso, Matilde
Rodriguez Cabello, José Carlos
González Vázquez, Arlyng
Planell, Josep A.
Engel, Elisabeth
Buján, Julia
Garcia-Honduvilla, Natalio
Vallet Regí, María Dulce Nombre
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 546
615.46
Bone repair
Tissue engineering
Elastin-like recombinamers (ELRs)
Silicon doped hydroxyapatite (Si-HA)
Rapid prototyped 3D scaffolds
Bone marrow Mesenchymal Stromal Cells (BMSCs)
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
topic 546
615.46
Bone repair
Tissue engineering
Elastin-like recombinamers (ELRs)
Silicon doped hydroxyapatite (Si-HA)
Rapid prototyped 3D scaffolds
Bone marrow Mesenchymal Stromal Cells (BMSCs)
Materiales
Química inorgánica (Química)
3312 Tecnología de Materiales
2303 Química Inorgánica
description The current study reports on the manufacturing by rapid prototyping technique of three-dimensional (3D) scaffolds based on silicon substituted hydroxyapatite with Elastin-like Recombinamers (ELRs) functionalized surfaces. Silicon doped hydroxyapatite (Si-HA), with Ca-10(PO4)(5.7)(SiO4)(0.3)(OH)(1.7)h(0.3) nominal formula, was surface functionalized with two different types of polymers designed by genetic engineering: ELR-RGD that contain cell attachment specific sequences and ELR-SNA15/RGD with both hydroxyapatite and cells domains that interact with the inorganic phase and with the cells, respectively. These hybrid materials were subjected to in vitro assays in order to clarify if the ELRs coating improved the well-known biocompatible and bone regeneration properties of calcium phosphates materials. The in vitro tests showed that there was a total and homogeneous colonization of the 3D scaffolds by Bone marrow Mesenchymal Stromal Cells (BMSCs). In addition, the BMSCs were viable and able to proliferate and differentiate into osteoblasts. Statement of Significance Bone tissue engineering is an area of increasing interest because its main applications are directly related to the rising life expectancy of the population, which promotes higher rates of several bone pathologies, so innovative strategies are needed for bone tissue regeneration therapies. Here we use the rapid prototyping technology to allow moulding ceramic 3D scaffolds and we use different bio-polymers for the functionalization of their surfaces in order to enhance the biological response. Combining the ceramic material (silicon doped hydroxyapatite, Si-HA) and the Elastin like Recombinamers (ELRs) polymers with the presence of the integrin-mediate adhesion domain alone or in combination with SNAI 5 peptide that possess high affinity for hydroxyapatite, provided an improved Bone marrow Mesenchymal Stromal Cells (BMSCs) differentiation into osteoblastic linkage. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/23100
url https://hdl.handle.net/20.500.14352/23100
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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