The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana
We have previously described a simple method to analyze the chromatin structure of Arabidopsis te- lomeres independently of that of Interstitial Telome- ric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/71033 |
| Acceso en línea: | https://hdl.handle.net/11441/71033 https://doi.org/10.4236/ajmb.2012.23025 |
| Access Level: | acceso abierto |
| Palabra clave: | Arabidopsis thaliana Heterochromatin ITSs Euchromatin Telomeres |
| Sumario: | We have previously described a simple method to analyze the chromatin structure of Arabidopsis te- lomeres independently of that of Interstitial Telome- ric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]. Some con- cerns have been recently raised about the accuracy of this procedure [2]. Here, we summarize these concerns and justify our experimental approaches and inter- pretation of results. |
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