Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc

Tesis doctoral inédita leída en la Universida Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 13-03-2015

Detalles Bibliográficos
Autor: Ramachandran, Gayetri
Tipo de recurso: tesis doctoral
Fecha de publicación:2015
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:español
inglés
OAI Identifier:oai:repositorio.uam.es:10486/666404
Acceso en línea:http://hdl.handle.net/10486/666404
Access Level:acceso abierto
Palabra clave:Bacilus subtilis - Tesis doctorales
Biología y Biomedicina / Biología
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spelling Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter PcRamachandran, GayetriBacilus subtilis - Tesis doctoralesBiología y Biomedicina / BiologíaTesis doctoral inédita leída en la Universida Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 13-03-2015Plasmid conjugation plays a significant role in the dissemination of antibiotic resistance and pathogenicity determinants. Understanding how conjugation is regulated is important to gain insights into these features. Little is known about regulation of conjugation systems present on plasmids from Gram-­‐positive bacteria. The plasmid pLS20 is a native conjugative plasmid isolated from Bacillus subtilis “natto”. Our earlier laboratory results reported a global view of the regulatory system of the pLS20 conjugation genes. It was found that about 40 conjugation genes were regulated by a divergently oriented single gene rcoLS20, which encodes for the master regulator of conjugation. RcoLS20 is responsible for keeping the system in its default “OFF” state. Conjugation is activated by anti-­‐repressor RapLS20, which belongs of the family of Rap proteins. However, the activity of RapLS20 is regulated by a signaling peptide Phr*LS20. Even though, the key players involved in the regulation of the conjugation genes have been identified, our knowledge on regulation of the genetic switch responsible for activating conjugation is limited. In the first chapter of the thesis, we have studied in detail the molecular mechanism regulating the pLS20 conjugation genes using different in vivo and in vitro approaches. At least three levels of regulation controls plasmid mediated-­‐ conjugation including overlapping and divergent promoter of differing strengths, maintaining the expression of the genes of conjugation and repression, along with a tri-­‐functional repressor RcoLS20, which regulates its own transcription and finally as a tetramer RcoLS20, by DNA-­‐looping. These complex regulatory levels permits the switching “OFF” and “ON” of the system of conjugation. The second chapter of the thesis studies the formation of the relaxosome of plasmid pLS20. This nucleoprotein is essential during conjugation, permitting the transfer of DNA. In case of plasmid pLS20, we found that besides the relaxase, encoded by gene 58 of pLS20, and that we named RelLS20, the functional processing of oriT requires two additional proteins encoded by genes 56 and 57.Meijer, Wihelmus J. J.Departamento de Biología MolecularFacultad de CienciasCentro de Biología Molecular Severo Ochoa (CBM)20152015-03-13doctoral thesishttp://purl.org/coar/resource_type/c_db06NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10486/666404reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridEspañolspaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6664042026-06-23T12:46:27Z
dc.title.none.fl_str_mv Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
title Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
spellingShingle Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
Ramachandran, Gayetri
Bacilus subtilis - Tesis doctorales
Biología y Biomedicina / Biología
title_short Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
title_full Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
title_fullStr Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
title_full_unstemmed Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
title_sort Functional characterization of the Bacillus subtilis conjugative plasmid pLS20: identification of relaxosome components and mechanism of transcriptional control of main conjugation promoter Pc
dc.creator.none.fl_str_mv Ramachandran, Gayetri
author Ramachandran, Gayetri
author_facet Ramachandran, Gayetri
author_role author
dc.contributor.none.fl_str_mv Meijer, Wihelmus J. J.
Departamento de Biología Molecular
Facultad de Ciencias
Centro de Biología Molecular Severo Ochoa (CBM)
dc.subject.none.fl_str_mv Bacilus subtilis - Tesis doctorales
Biología y Biomedicina / Biología
topic Bacilus subtilis - Tesis doctorales
Biología y Biomedicina / Biología
description Tesis doctoral inédita leída en la Universida Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 13-03-2015
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-03-13
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/666404
url http://hdl.handle.net/10486/666404
dc.language.none.fl_str_mv Español
spa
Inglés
eng
language_invalid_str_mv Español
Inglés
language spa
eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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