Differential effects of carbohydrates on arabidopsis pollen germination

Pollen germination as a crucial process in plant development strongly depends on the accessibility of carbon as energy source. Carbohydrates, however, function not only as a primary energy source, but also as important signaling components. In a comprehensive study, we analyzed various aspects of th...

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Autores: Hirsche, Jörg, García Fernández, José Manuel, Stabentheiner, Edith, Großkinsky, Dominik K., Roitsch, Thomas Georg
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/102490
Acceso en línea:https://hdl.handle.net/11441/102490
https://doi.org/10.1093/pcp/pcx020
Access Level:acceso abierto
Palabra clave:Arabidopsis thaliana
Carbohydrates
Metabolic regulation
Pollen germination
Signaling
Structure-function relationship
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spelling Differential effects of carbohydrates on arabidopsis pollen germinationHirsche, JörgGarcía Fernández, José ManuelStabentheiner, EdithGroßkinsky, Dominik K.Roitsch, Thomas GeorgArabidopsis thalianaCarbohydratesMetabolic regulationPollen germinationSignalingStructure-function relationshipPollen germination as a crucial process in plant development strongly depends on the accessibility of carbon as energy source. Carbohydrates, however, function not only as a primary energy source, but also as important signaling components. In a comprehensive study, we analyzed various aspects of the impact of 32 different sugars on in vitro germination of Arabidopsis pollen comprising about 150 variations of individual sugars and combinations. Twenty-six structurally different mono-, di- and oligosaccharides, and sugar analogs were initially tested for their ability to support pollen germination. Whereas several di- and oligosaccharides supported pollen germination, hexoses such as glucose, fructose and mannose did not support and even considerably inhibited pollen germination when added to germination-supporting medium. Complementary experiments using glucose analogs with varying functional features, the hexokinase inhibitor mannoheptulose and the glucose-insensitive hexokinase-deficient Arabidopsis mutant gin2-1 suggested that mannose- and glucose-mediated inhibition of sucrose-supported pollen germination depends partially on hexokinase signaling. The results suggest that, in addition to their role as energy source, sugars act as signaling molecules differentially regulating the complex process of pollen germination depending on their structural properties. Thus, a sugar-dependent multilayer regulation of Arabidopsis pollen germination is supported, which makes this approach a valuable experimental system for future studies addressing sugar sensing and signaling.Ministerio de Economía y Competitividad CTQ2015-64425-C2-1-RJunta de Andalucía FQM2012-1467Oxford University Press2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/102490https://doi.org/10.1093/pcp/pcx020reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPlant and Cell Physiology, 58 (4), 691-701.CTQ2015-64425-C2-1-RFQM2012-1467https://doi.org/10.1093/pcp/pcx020info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1024902026-06-17T12:51:07Z
dc.title.none.fl_str_mv Differential effects of carbohydrates on arabidopsis pollen germination
title Differential effects of carbohydrates on arabidopsis pollen germination
spellingShingle Differential effects of carbohydrates on arabidopsis pollen germination
Hirsche, Jörg
Arabidopsis thaliana
Carbohydrates
Metabolic regulation
Pollen germination
Signaling
Structure-function relationship
title_short Differential effects of carbohydrates on arabidopsis pollen germination
title_full Differential effects of carbohydrates on arabidopsis pollen germination
title_fullStr Differential effects of carbohydrates on arabidopsis pollen germination
title_full_unstemmed Differential effects of carbohydrates on arabidopsis pollen germination
title_sort Differential effects of carbohydrates on arabidopsis pollen germination
dc.creator.none.fl_str_mv Hirsche, Jörg
García Fernández, José Manuel
Stabentheiner, Edith
Großkinsky, Dominik K.
Roitsch, Thomas Georg
author Hirsche, Jörg
author_facet Hirsche, Jörg
García Fernández, José Manuel
Stabentheiner, Edith
Großkinsky, Dominik K.
Roitsch, Thomas Georg
author_role author
author2 García Fernández, José Manuel
Stabentheiner, Edith
Großkinsky, Dominik K.
Roitsch, Thomas Georg
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis thaliana
Carbohydrates
Metabolic regulation
Pollen germination
Signaling
Structure-function relationship
topic Arabidopsis thaliana
Carbohydrates
Metabolic regulation
Pollen germination
Signaling
Structure-function relationship
description Pollen germination as a crucial process in plant development strongly depends on the accessibility of carbon as energy source. Carbohydrates, however, function not only as a primary energy source, but also as important signaling components. In a comprehensive study, we analyzed various aspects of the impact of 32 different sugars on in vitro germination of Arabidopsis pollen comprising about 150 variations of individual sugars and combinations. Twenty-six structurally different mono-, di- and oligosaccharides, and sugar analogs were initially tested for their ability to support pollen germination. Whereas several di- and oligosaccharides supported pollen germination, hexoses such as glucose, fructose and mannose did not support and even considerably inhibited pollen germination when added to germination-supporting medium. Complementary experiments using glucose analogs with varying functional features, the hexokinase inhibitor mannoheptulose and the glucose-insensitive hexokinase-deficient Arabidopsis mutant gin2-1 suggested that mannose- and glucose-mediated inhibition of sucrose-supported pollen germination depends partially on hexokinase signaling. The results suggest that, in addition to their role as energy source, sugars act as signaling molecules differentially regulating the complex process of pollen germination depending on their structural properties. Thus, a sugar-dependent multilayer regulation of Arabidopsis pollen germination is supported, which makes this approach a valuable experimental system for future studies addressing sugar sensing and signaling.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/102490
https://doi.org/10.1093/pcp/pcx020
url https://hdl.handle.net/11441/102490
https://doi.org/10.1093/pcp/pcx020
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plant and Cell Physiology, 58 (4), 691-701.
CTQ2015-64425-C2-1-R
FQM2012-1467
https://doi.org/10.1093/pcp/pcx020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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