Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis

BACKGROUND: Canine leishmaniasis (CanL) is a severe chronic disease caused by Leishmania infantum and transmitted by sand flies of which the main vector in the Western part of the Mediterranean basin is Phlebotomus perniciosus. Previously, an immunochromatographic test (ICT) was proposed to allow ra...

Descripción completa

Detalles Bibliográficos
Autores: Willen, Laura, Lestinova, Tereza, Kalousková, Barbora, Sumova, Petra, Spitzova, Tatiana, Velez, Maria Rita Perdigão, Domènech Viñolas, Ester, Vaněk, Ondřej, Gállego Culleré, M. (Montserrat), Mertens, Pascal, Volf, Petr
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/147806
Acceso en línea:https://hdl.handle.net/2445/147806
Access Level:acceso abierto
Palabra clave:Leishmaniosi
Patologia veterinària
Leishmaniasis
Veterinary pathology
id ES_10fa30445b14db97dbf1a7690cddd4df
oai_identifier_str oai:diposit.ub.edu:2445/147806
network_acronym_str ES
network_name_str España
repository_id_str
spelling Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasisWillen, LauraLestinova, TerezaKalousková, BarboraSumova, PetraSpitzova, TatianaVelez, Maria Rita PerdigãoDomènech Viñolas, EsterVaněk, OndřejGállego Culleré, M. (Montserrat)Mertens, PascalVolf, PetrLeishmaniosiPatologia veterinàriaLeishmaniasisVeterinary pathologyBACKGROUND: Canine leishmaniasis (CanL) is a severe chronic disease caused by Leishmania infantum and transmitted by sand flies of which the main vector in the Western part of the Mediterranean basin is Phlebotomus perniciosus. Previously, an immunochromatographic test (ICT) was proposed to allow rapid evaluation of dog exposure to P. perniciosus. In the present study, we optimized the prototype and evaluated the detection accuracy of the ICT in field conditions. Possible cross-reactions with other hematophagous arthropods were also assessed. METHODOLOGY/PRINCIPAL FINDINGS: The ICT was optimized by expressing the rSP03B protein in a HEK293 cell line, which delivered an increased specificity (94.92%). The ICT showed an excellent reproducibility and inter-person reliability, and was optimized for use with whole canine blood which rendered an excellent degree of agreement with the use of serum. Field detectability of the ICT was assessed by screening 186 dogs from different CanL endemic areas with both the SGH-ELISA and the ICT, and 154 longitudinally sampled dogs only with the ICT. The ICT results corresponded to the SGH-ELISA for most areas, depending on the statistical measure used. Furthermore, the ICT was able to show a clear seasonal fluctuation in the proportion of bitten dogs. Finally, we excluded cross-reactions between non-vector species and confirmed favorable cross-reactions with other L. infantum vectors belonging to the subgenus Larroussius. CONCLUSIONS/SIGNIFICANCE: We have successfully optimized the ICT, now also suitable to be used with whole canine blood. The test is able to reflect the seasonal fluctuation in dog exposure and showed a good detectability in a field population of naturally exposed dogs, particularly in areas with a high seroprevalence of bitten dogs. Furthermore, our study showed the existence of favorable cross-reactions with other sand fly vectors thereby expanding its use in the field.Public Library of Science (PLoS)2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/147806Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1371/journal.pntd.0007832PLoS Neglected Tropical Diseases, 2019, vol. 13, num. 11, p. e0007832https://doi.org/10.1371/journal.pntd.0007832info:eu-repo/grantAgreement/EC/H2020/642609cc-by (c) Willen, Laura et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1478062026-05-27T06:46:51Z
dc.title.none.fl_str_mv Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
title Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
spellingShingle Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
Willen, Laura
Leishmaniosi
Patologia veterinària
Leishmaniasis
Veterinary pathology
title_short Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
title_full Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
title_fullStr Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
title_full_unstemmed Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
title_sort Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis
dc.creator.none.fl_str_mv Willen, Laura
Lestinova, Tereza
Kalousková, Barbora
Sumova, Petra
Spitzova, Tatiana
Velez, Maria Rita Perdigão
Domènech Viñolas, Ester
Vaněk, Ondřej
Gállego Culleré, M. (Montserrat)
Mertens, Pascal
Volf, Petr
author Willen, Laura
author_facet Willen, Laura
Lestinova, Tereza
Kalousková, Barbora
Sumova, Petra
Spitzova, Tatiana
Velez, Maria Rita Perdigão
Domènech Viñolas, Ester
Vaněk, Ondřej
Gállego Culleré, M. (Montserrat)
Mertens, Pascal
Volf, Petr
author_role author
author2 Lestinova, Tereza
Kalousková, Barbora
Sumova, Petra
Spitzova, Tatiana
Velez, Maria Rita Perdigão
Domènech Viñolas, Ester
Vaněk, Ondřej
Gállego Culleré, M. (Montserrat)
Mertens, Pascal
Volf, Petr
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Leishmaniosi
Patologia veterinària
Leishmaniasis
Veterinary pathology
topic Leishmaniosi
Patologia veterinària
Leishmaniasis
Veterinary pathology
description BACKGROUND: Canine leishmaniasis (CanL) is a severe chronic disease caused by Leishmania infantum and transmitted by sand flies of which the main vector in the Western part of the Mediterranean basin is Phlebotomus perniciosus. Previously, an immunochromatographic test (ICT) was proposed to allow rapid evaluation of dog exposure to P. perniciosus. In the present study, we optimized the prototype and evaluated the detection accuracy of the ICT in field conditions. Possible cross-reactions with other hematophagous arthropods were also assessed. METHODOLOGY/PRINCIPAL FINDINGS: The ICT was optimized by expressing the rSP03B protein in a HEK293 cell line, which delivered an increased specificity (94.92%). The ICT showed an excellent reproducibility and inter-person reliability, and was optimized for use with whole canine blood which rendered an excellent degree of agreement with the use of serum. Field detectability of the ICT was assessed by screening 186 dogs from different CanL endemic areas with both the SGH-ELISA and the ICT, and 154 longitudinally sampled dogs only with the ICT. The ICT results corresponded to the SGH-ELISA for most areas, depending on the statistical measure used. Furthermore, the ICT was able to show a clear seasonal fluctuation in the proportion of bitten dogs. Finally, we excluded cross-reactions between non-vector species and confirmed favorable cross-reactions with other L. infantum vectors belonging to the subgenus Larroussius. CONCLUSIONS/SIGNIFICANCE: We have successfully optimized the ICT, now also suitable to be used with whole canine blood. The test is able to reflect the seasonal fluctuation in dog exposure and showed a good detectability in a field population of naturally exposed dogs, particularly in areas with a high seroprevalence of bitten dogs. Furthermore, our study showed the existence of favorable cross-reactions with other sand fly vectors thereby expanding its use in the field.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/147806
url https://hdl.handle.net/2445/147806
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pntd.0007832
PLoS Neglected Tropical Diseases, 2019, vol. 13, num. 11, p. e0007832
https://doi.org/10.1371/journal.pntd.0007832
info:eu-repo/grantAgreement/EC/H2020/642609
dc.rights.none.fl_str_mv cc-by (c) Willen, Laura et al., 2019
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Willen, Laura et al., 2019
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403540054605824
score 15,301629

Similares en LA Referencia