Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain

Background: Canine leishmaniosis caused by Leishmania infantum is a neglected zoonosis transmitted by sand flies like Phlebotomus perniciosus. Clinical signs and disease susceptibility vary according to various factors, including host immune response and breed. In particular, Ibizan hounds appear mo...

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Autores: Burnham, Alexis C., Ordeix, Laura, Alcover Amengual, Maria Magdalena, Martínez-Orellana, Pamela, Montserrat-Sangrà, Sara, Willen, Laura, Spitzova, Tatiana, Volf, Petr, Solano-Gallego, Laia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/159937
Acesso em linha:https://hdl.handle.net/2445/159937
Access Level:acceso abierto
Palavra-chave:Leishmania infantum
Saliva
Dermatitis
Gossos
Patologia veterinària
Eivissa (Illes Balears)
Dogs
Veterinary pathology
Ibiza (Balearic Islands)
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spelling Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, SpainBurnham, Alexis C.Ordeix, LauraAlcover Amengual, Maria MagdalenaMartínez-Orellana, PamelaMontserrat-Sangrà, SaraWillen, LauraSpitzova, TatianaVolf, PetrSolano-Gallego, LaiaLeishmania infantumSalivaDermatitisGossosPatologia veterinàriaEivissa (Illes Balears)Leishmania infantumSalivaDermatitisDogsVeterinary pathologyIbiza (Balearic Islands)Background: Canine leishmaniosis caused by Leishmania infantum is a neglected zoonosis transmitted by sand flies like Phlebotomus perniciosus. Clinical signs and disease susceptibility vary according to various factors, including host immune response and breed. In particular, Ibizan hounds appear more resistant. This immunocompetence could be attributed to a more frequent exposure to uninfected sand flies, eliciting a stronger anti-sand fly saliva antibody response. Methods: This study aimed to investigate the prevalence of anti-P. perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in the Leishmania-endemic area of Mallorca, Spain, and to correlate these antibody levels with clinical, immunological and parasitological parameters. Anti-sand fly saliva IgG was examined in 47 Ibizan hounds and 45 dogs of other breeds using three methods: P. perniciosus whole salivary gland homogenate (SGH) ELISA; recombinant protein rSP03B ELISA; and rSP03B rapid tests (RT). Additionally, diagnostic performance was evaluated between methods. Results: Results indicate significantly higher anti-SGH antibodies (P = 0.0061) and a trend for more positive SGH ELISA and RT results in Ibizan hounds compared to other breeds. General linear model analysis also found breed to be a significant factor in SGH ELISA units and a marginally significant factor in RT result. Although infection rates were similar between groups, Ibizan hounds included significantly more IFN-γ producers (P = 0.0122) and papular dermatitis cases (P < 0.0001). Older age and L. infantum seropositivity were also considered significant factors in sand fly saliva antibody levels according to at least one test. Fair agreement was found between all three tests, with the highest value between SGH and rSP03B RT. Conclusions: To our knowledge, this is the first study elaborating the relationship between anti-P. perniciosus saliva antibodies and extensive clinical data in dogs in an endemic area. Our results suggest that Ibizan hounds experience a higher frequency of exposure to sand flies and have a stronger cellular immune response to L. infantum infection than other breed dogs. Additional sampling is needed to confirm results, but anti-P. perniciosus saliva antibodies appear to negatively correlate with susceptibility to L. infantum infection and could possibly contribute to the resistance observed in Ibizan hounds.BioMed Central2020202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/2445/159937Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1186/s13071-020-3992-8Parasites & Vectors, 2020, vol. 13(1), num. 129https://doi.org/10.1186/s13071-020-3992-8cc-by (c) Burnham, Alexis C. et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1599372026-05-29T05:05:01Z
dc.title.none.fl_str_mv Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
title Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
spellingShingle Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
Burnham, Alexis C.
Leishmania infantum
Saliva
Dermatitis
Gossos
Patologia veterinària
Eivissa (Illes Balears)
Leishmania infantum
Saliva
Dermatitis
Dogs
Veterinary pathology
Ibiza (Balearic Islands)
title_short Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
title_full Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
title_fullStr Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
title_full_unstemmed Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
title_sort Exploring the Relationship Between Susceptibility to Canine Leishmaniosis and anti-Phlebotomus Perniciosus Saliva Antibodies in Ibizan Hounds and Dogs of Other Breeds in Mallorca, Spain
dc.creator.none.fl_str_mv Burnham, Alexis C.
Ordeix, Laura
Alcover Amengual, Maria Magdalena
Martínez-Orellana, Pamela
Montserrat-Sangrà, Sara
Willen, Laura
Spitzova, Tatiana
Volf, Petr
Solano-Gallego, Laia
author Burnham, Alexis C.
author_facet Burnham, Alexis C.
Ordeix, Laura
Alcover Amengual, Maria Magdalena
Martínez-Orellana, Pamela
Montserrat-Sangrà, Sara
Willen, Laura
Spitzova, Tatiana
Volf, Petr
Solano-Gallego, Laia
author_role author
author2 Ordeix, Laura
Alcover Amengual, Maria Magdalena
Martínez-Orellana, Pamela
Montserrat-Sangrà, Sara
Willen, Laura
Spitzova, Tatiana
Volf, Petr
Solano-Gallego, Laia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Leishmania infantum
Saliva
Dermatitis
Gossos
Patologia veterinària
Eivissa (Illes Balears)
Leishmania infantum
Saliva
Dermatitis
Dogs
Veterinary pathology
Ibiza (Balearic Islands)
topic Leishmania infantum
Saliva
Dermatitis
Gossos
Patologia veterinària
Eivissa (Illes Balears)
Leishmania infantum
Saliva
Dermatitis
Dogs
Veterinary pathology
Ibiza (Balearic Islands)
description Background: Canine leishmaniosis caused by Leishmania infantum is a neglected zoonosis transmitted by sand flies like Phlebotomus perniciosus. Clinical signs and disease susceptibility vary according to various factors, including host immune response and breed. In particular, Ibizan hounds appear more resistant. This immunocompetence could be attributed to a more frequent exposure to uninfected sand flies, eliciting a stronger anti-sand fly saliva antibody response. Methods: This study aimed to investigate the prevalence of anti-P. perniciosus saliva antibodies in Ibizan hounds and dogs of other breeds in the Leishmania-endemic area of Mallorca, Spain, and to correlate these antibody levels with clinical, immunological and parasitological parameters. Anti-sand fly saliva IgG was examined in 47 Ibizan hounds and 45 dogs of other breeds using three methods: P. perniciosus whole salivary gland homogenate (SGH) ELISA; recombinant protein rSP03B ELISA; and rSP03B rapid tests (RT). Additionally, diagnostic performance was evaluated between methods. Results: Results indicate significantly higher anti-SGH antibodies (P = 0.0061) and a trend for more positive SGH ELISA and RT results in Ibizan hounds compared to other breeds. General linear model analysis also found breed to be a significant factor in SGH ELISA units and a marginally significant factor in RT result. Although infection rates were similar between groups, Ibizan hounds included significantly more IFN-γ producers (P = 0.0122) and papular dermatitis cases (P < 0.0001). Older age and L. infantum seropositivity were also considered significant factors in sand fly saliva antibody levels according to at least one test. Fair agreement was found between all three tests, with the highest value between SGH and rSP03B RT. Conclusions: To our knowledge, this is the first study elaborating the relationship between anti-P. perniciosus saliva antibodies and extensive clinical data in dogs in an endemic area. Our results suggest that Ibizan hounds experience a higher frequency of exposure to sand flies and have a stronger cellular immune response to L. infantum infection than other breed dogs. Additional sampling is needed to confirm results, but anti-P. perniciosus saliva antibodies appear to negatively correlate with susceptibility to L. infantum infection and could possibly contribute to the resistance observed in Ibizan hounds.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/159937
url https://hdl.handle.net/2445/159937
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/s13071-020-3992-8
Parasites & Vectors, 2020, vol. 13(1), num. 129
https://doi.org/10.1186/s13071-020-3992-8
dc.rights.none.fl_str_mv cc-by (c) Burnham, Alexis C. et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Burnham, Alexis C. et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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