Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall

19 páginas, 6 figuras, 1 tabla

Detalles Bibliográficos
Autores: Serrat, Judit, Torres Valle, María, López-García, Marta, Becerro Recio, David, Siles Lucas, Mar, González Miguel, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/311044
Acceso en línea:http://hdl.handle.net/10261/311044
https://api.elsevier.com/content/abstract/scopus_id/85159353244
Access Level:acceso abierto
Palabra clave:Fasciola hepatica newly excysted juveniles
Adhesion
Extracellular matrix
Fasciolosis
Host–parasite relationships
Laminin
Migration
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dc.title.none.fl_str_mv Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
title Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
spellingShingle Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
Serrat, Judit
Fasciola hepatica newly excysted juveniles
Adhesion
Extracellular matrix
Fasciolosis
Host–parasite relationships
Laminin
Migration
title_short Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
title_full Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
title_fullStr Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
title_full_unstemmed Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
title_sort Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wall
dc.creator.none.fl_str_mv Serrat, Judit
Torres Valle, María
López-García, Marta
Becerro Recio, David
Siles Lucas, Mar
González Miguel, Javier
author Serrat, Judit
author_facet Serrat, Judit
Torres Valle, María
López-García, Marta
Becerro Recio, David
Siles Lucas, Mar
González Miguel, Javier
author_role author
author2 Torres Valle, María
López-García, Marta
Becerro Recio, David
Siles Lucas, Mar
González Miguel, Javier
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Junta de Castilla y León
Serrat, Judit [0000-0002-1182-1088]
López García, Marta [0000-0002-7412-4597]
Siles Lucas, Mar [0000-0002-1257-2562]
González Miguel, Javier [0000-0003-4279-4761]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fasciola hepatica newly excysted juveniles
Adhesion
Extracellular matrix
Fasciolosis
Host–parasite relationships
Laminin
Migration
topic Fasciola hepatica newly excysted juveniles
Adhesion
Extracellular matrix
Fasciolosis
Host–parasite relationships
Laminin
Migration
description 19 páginas, 6 figuras, 1 tabla
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/311044
https://api.elsevier.com/content/abstract/scopus_id/85159353244
url http://hdl.handle.net/10261/311044
https://api.elsevier.com/content/abstract/scopus_id/85159353244
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093463-J-100
Serrat, Judit (2025). The role of the fibrinolytic system in the migration of Fasciola hepatica juveniles across the host intestine. http://hdl.handle.net/10261/414641
http://dx.doi.org/10.3390/ijms24098165
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Molecular characterization of the interplay between Fasciola hepatica juveniles and laminin as a mechanism to adhere to and break through the host intestinal wallSerrat, JuditTorres Valle, MaríaLópez-García, MartaBecerro Recio, DavidSiles Lucas, MarGonzález Miguel, JavierFasciola hepatica newly excysted juvenilesAdhesionExtracellular matrixFasciolosisHost–parasite relationshipsLamininMigration19 páginas, 6 figuras, 1 tablaFasciola hepatica is the main causative agent of fasciolosis, a zoonotic parasitic disease of growing public health concern. F. hepatica metacercariae are ingested by the host and excyst in the intestine, thereby releasing the newly excysted juveniles (FhNEJ), which traverse the gut wall and migrate towards the biliary ducts. Since blocking F. hepatica development is challenging after crossing of the intestinal wall, targeting this first step of migration might result in increased therapeutic success. The intestinal extracellular matrix (ECM) is constituted by a network of structural proteins, including laminin (LM) and fibronectin (FN), that provide mechanical support while acting as physical barrier against intestinal pathogens. Here, we employed ELISA and immunofluorescent assays to test for the presence of LM- and FN-binding proteins on a tegument-enriched antigenic fraction of FhNEJ, and further determined their identity by two-dimensional electrophoresis coupled to mass spectrometry. Additionally, we performed enzymatic assays that revealed for the first time the capability of the juvenile-specific cathepsin L3 to degrade LM, and that LM degradation by FhNEJ proteins is further potentiated in the presence of host plasminogen. Finally, a proteomic analysis showed that the interaction with LM triggers protein changes in FhNEJ that may be relevant for parasite growth and adaptation inside the mammalian host. Altogether, our study provides valuable insights into the molecular interplay between FhNEJ and the intestinal ECM, which may lead to the identification of targetable candidates for the development of more effective control strategies against fasciolosis.This work was supported by the JIN project ‘ULYSSES’ (RTI2018-093463-J-100) funded by the Ministerio de Ciencia, Innovación y Universidades (MCIU), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER, UE). We also acknowledge the financial support of the project “CLU-2019-05–IRNASA-CSIC Unit of Excellence”, funded by the Junta de Castilla y León and co-funded by the European Union (FEDER “Europe drives our growth”), and the Programme for strengthening research structures “Stairway to excellence” internationalisation aid, co-funded by the European Regional Development Fund. M.S.L. acknowledges the financial support of the Spanish Ministry of Science and Innovation (Project PID2019-108782RB-C22). J.S. and D.B.R. acknowledge the support of the Junta de Castilla y León for their Predoctoral contracts. M.L.G. acknowledges the support of the Spanish Ministry of Science and Innovation for her FPU Predoctoral contract. J.G.M. is supported by the ‘Ramón y Cajal’ program (RYC2020-030575-I) of the Ministerio de Ciencia e Innovación. The proteomics laboratory where LC-MS/MS analysis was performed is a member of Proteored, PRB3 and is supported by grant PT17/0019, of the PE I + D+i 2013–2016, funded by the Instituto de Salud Carlos III (ISCIII) and the European Regional Development Fund (ERDF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscriptPeer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Junta de Castilla y LeónSerrat, Judit [0000-0002-1182-1088]López García, Marta [0000-0002-7412-4597]Siles Lucas, Mar [0000-0002-1257-2562]González Miguel, Javier [0000-0003-4279-4761]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/311044https://api.elsevier.com/content/abstract/scopus_id/85159353244reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093463-J-100Serrat, Judit (2025). The role of the fibrinolytic system in the migration of Fasciola hepatica juveniles across the host intestine. http://hdl.handle.net/10261/414641http://dx.doi.org/10.3390/ijms24098165Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3110442026-05-22T06:33:51Z
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