Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea

Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with p...

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Detalles Bibliográficos
Autores: Tzlil, Gali, Molina Márquez, Ana María, León Bañares, Rosa María, Béjà, Oded
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad de Huelva (UHU)
Repositorio:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Idioma:inglés
OAI Identifier:oai:ariasmontano.uhu.es:10272/27517
Acceso en línea:https://hdl.handle.net/10272/27517
Access Level:acceso abierto
Palabra clave:2302 Bioquímica
2414 Microbiología
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spelling Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaeaTzlil, GaliMolina Márquez, Ana MaríaLeón Bañares, Rosa MaríaBéjà, Oded2302 Bioquímica2414 MicrobiologíaAquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of ‘Candidatus Kariarchaeaceae’ (‘Candidatus Asgardarchaeota’). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea.Nature Research20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10272/27517reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/275172026-06-02T14:58:11Z
dc.title.none.fl_str_mv Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
title Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
spellingShingle Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
Tzlil, Gali
2302 Bioquímica
2414 Microbiología
title_short Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
title_full Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
title_fullStr Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
title_full_unstemmed Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
title_sort Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea
dc.creator.none.fl_str_mv Tzlil, Gali
Molina Márquez, Ana María
León Bañares, Rosa María
Béjà, Oded
author Tzlil, Gali
author_facet Tzlil, Gali
Molina Márquez, Ana María
León Bañares, Rosa María
Béjà, Oded
author_role author
author2 Molina Márquez, Ana María
León Bañares, Rosa María
Béjà, Oded
author2_role author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv 2302 Bioquímica
2414 Microbiología
topic 2302 Bioquímica
2414 Microbiología
description Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of ‘Candidatus Kariarchaeaceae’ (‘Candidatus Asgardarchaeota’). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10272/27517
url https://hdl.handle.net/10272/27517
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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