High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome

In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited infor...

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Authors: López Guimet, Júlia, Andilla i Salla, Jordi, Loza Álvarez, Pablo, Egea Guri, Gustavo
Format: article
Status:Published version
Publication Date:2017
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/126543
Online Access:https://hdl.handle.net/2445/126543
Access Level:Open access
Keyword:Malalties hereditàries
Malalties del teixit connectiu
Aneurismes aòrtics
Genetic diseases
Connective tissues diseases
Aortic aneurysms
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spelling High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan SyndromeLópez Guimet, JúliaAndilla i Salla, JordiLoza Álvarez, PabloEgea Guri, GustavoMalalties hereditàriesMalalties del teixit connectiuAneurismes aòrticsGenetic diseasesConnective tissues diseasesAortic aneurysmsIn Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited information about the precise location, progression and 3D extension of the microstructural changes that occur in each lamina. We implemented a method using multiphoton excitation fluorescence microscopy and computational image processing, which provides high-resolution en-face images of segmented individual laminae from unstained whole aortic samples. We showed that internal elastic laminae and successive 2(nd) laminae are injured to a different extent in murine Marfan aortae; in particular, the density and size of fenestrae changed. Moreover, microstructural injuries were concentrated in the aortic proximal and convex anatomical regions. Other parameters such as the waviness and thickness of each lamina remained unaltered. In conclusion, the method reported here is a useful, unique tool for en-face laminae microstructure assessment that can obtain quantitative three-dimensional information about vascular tissue. The application of this method to murine Marfan aortae clearly shows that the microstructural damage in elastic laminae is not equal throughout the thickness of the tunica media and in the different anatomical regions of the ascending aorta.Nature Publishing Group2018201820172018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/126543Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-017-01620-8Scientific Reports, 2017, vol. 7https://doi.org/10.1038/s41598-017-01620-8info:eu-repo/grantAgreement/EC/H2020/654148cc-by (c) Lopez-Guimet, Julia et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1265432026-05-29T05:05:01Z
dc.title.none.fl_str_mv High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
title High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
spellingShingle High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
López Guimet, Júlia
Malalties hereditàries
Malalties del teixit connectiu
Aneurismes aòrtics
Genetic diseases
Connective tissues diseases
Aortic aneurysms
title_short High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
title_full High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
title_fullStr High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
title_full_unstemmed High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
title_sort High-Resolution morphological approach to analyse elastic laminae injuries of the ascending aorta in a murine model of Marfan Syndrome
dc.creator.none.fl_str_mv López Guimet, Júlia
Andilla i Salla, Jordi
Loza Álvarez, Pablo
Egea Guri, Gustavo
author López Guimet, Júlia
author_facet López Guimet, Júlia
Andilla i Salla, Jordi
Loza Álvarez, Pablo
Egea Guri, Gustavo
author_role author
author2 Andilla i Salla, Jordi
Loza Álvarez, Pablo
Egea Guri, Gustavo
author2_role author
author
author
dc.subject.none.fl_str_mv Malalties hereditàries
Malalties del teixit connectiu
Aneurismes aòrtics
Genetic diseases
Connective tissues diseases
Aortic aneurysms
topic Malalties hereditàries
Malalties del teixit connectiu
Aneurismes aòrtics
Genetic diseases
Connective tissues diseases
Aortic aneurysms
description In Marfan syndrome, the tunica media is disrupted, which leads to the formation of ascending aortic aneurysms. Marfan aortic samples are histologically characterized by the fragmentation of elastic laminae. However, conventional histological techniques using transverse sections provide limited information about the precise location, progression and 3D extension of the microstructural changes that occur in each lamina. We implemented a method using multiphoton excitation fluorescence microscopy and computational image processing, which provides high-resolution en-face images of segmented individual laminae from unstained whole aortic samples. We showed that internal elastic laminae and successive 2(nd) laminae are injured to a different extent in murine Marfan aortae; in particular, the density and size of fenestrae changed. Moreover, microstructural injuries were concentrated in the aortic proximal and convex anatomical regions. Other parameters such as the waviness and thickness of each lamina remained unaltered. In conclusion, the method reported here is a useful, unique tool for en-face laminae microstructure assessment that can obtain quantitative three-dimensional information about vascular tissue. The application of this method to murine Marfan aortae clearly shows that the microstructural damage in elastic laminae is not equal throughout the thickness of the tunica media and in the different anatomical regions of the ascending aorta.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/126543
url https://hdl.handle.net/2445/126543
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-017-01620-8
Scientific Reports, 2017, vol. 7
https://doi.org/10.1038/s41598-017-01620-8
info:eu-repo/grantAgreement/EC/H2020/654148
dc.rights.none.fl_str_mv cc-by (c) Lopez-Guimet, Julia et al., 2017
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Lopez-Guimet, Julia et al., 2017
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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