Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration

Limbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have...

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Autores: Nieto-Nicolau, Núria|||0000-0002-3527-0110, Martínez-Conesa, Eva M.|||0000-0002-3696-7702, Fuentes-Julián, S., Arnalich-Montiel, Francisco, García-Tuñón, I., De Miguel, M.P., Casaroli-Marano, Ricardo Pedro|||0000-0003-1812-9323
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:269725
Acceso en línea:https://ddd.uab.cat/record/269725
https://dx.doi.org/urn:doi:10.1111/jcmm.16501
Access Level:acceso abierto
Palabra clave:Cell culture priming
Cytokines
Directed differentiation media
Limbal stem cells
Limbal stem cells deficiency
Paracrine secretion
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spelling Priming human adipose-derived mesenchymal stem cells for corneal surface regenerationNieto-Nicolau, Núria|||0000-0002-3527-0110Martínez-Conesa, Eva M.|||0000-0002-3696-7702Fuentes-Julián, S.Arnalich-Montiel, FranciscoGarcía-Tuñón, I.De Miguel, M.P.Casaroli-Marano, Ricardo Pedro|||0000-0003-1812-9323Cell culture primingCytokinesDirected differentiation mediaLimbal stem cellsLimbal stem cells deficiencyParacrine secretionLimbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have shown promise in animal models to treat LSC deficiency (LSCD), but there are not studies showing their efficacy when primed with different media before transplantation. We cultured AT-MSC with standard medium and media used to culture LSC for clinical application. We demonstrated that different media changed the AT-MSC paracrine secretion showing different paracrine effector functions in an in vivo model of chemical burn and in response to a novel in vitro model of corneal inflammation by alkali induction. Treatment of LSCD with AT-MSC changed the angiogenic and inflammatory cytokine profile of mice corneas. AT-MSC cultured with the medium that improved their cytokine secretion, enhanced the anti-angiogenic and anti-inflammatory profile of the treated corneas. Those corneas also presented better outcome in terms of corneal transparency, neovascularization and histologic reconstruction. Priming human AT-MSC with LSC specific medium can potentiate their ability to improve corneal wound healing, decrease neovascularization and inflammation modulating paracrine effector functions in an in vivo optimized rat model of LSCD. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/269725https://dx.doi.org/urn:doi:10.1111/jcmm.16501reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 FIS14-PI00196Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FIS18-PI00355Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI15-01447Ministerio de Educación, Cultura y Deporte https://doi.org/10.13039/501100003176 AP2010-0659open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2697252026-06-06T12:50:31Z
dc.title.none.fl_str_mv Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
title Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
spellingShingle Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
Nieto-Nicolau, Núria|||0000-0002-3527-0110
Cell culture priming
Cytokines
Directed differentiation media
Limbal stem cells
Limbal stem cells deficiency
Paracrine secretion
title_short Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
title_full Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
title_fullStr Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
title_full_unstemmed Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
title_sort Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration
dc.creator.none.fl_str_mv Nieto-Nicolau, Núria|||0000-0002-3527-0110
Martínez-Conesa, Eva M.|||0000-0002-3696-7702
Fuentes-Julián, S.
Arnalich-Montiel, Francisco
García-Tuñón, I.
De Miguel, M.P.
Casaroli-Marano, Ricardo Pedro|||0000-0003-1812-9323
author Nieto-Nicolau, Núria|||0000-0002-3527-0110
author_facet Nieto-Nicolau, Núria|||0000-0002-3527-0110
Martínez-Conesa, Eva M.|||0000-0002-3696-7702
Fuentes-Julián, S.
Arnalich-Montiel, Francisco
García-Tuñón, I.
De Miguel, M.P.
Casaroli-Marano, Ricardo Pedro|||0000-0003-1812-9323
author_role author
author2 Martínez-Conesa, Eva M.|||0000-0002-3696-7702
Fuentes-Julián, S.
Arnalich-Montiel, Francisco
García-Tuñón, I.
De Miguel, M.P.
Casaroli-Marano, Ricardo Pedro|||0000-0003-1812-9323
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell culture priming
Cytokines
Directed differentiation media
Limbal stem cells
Limbal stem cells deficiency
Paracrine secretion
topic Cell culture priming
Cytokines
Directed differentiation media
Limbal stem cells
Limbal stem cells deficiency
Paracrine secretion
description Limbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have shown promise in animal models to treat LSC deficiency (LSCD), but there are not studies showing their efficacy when primed with different media before transplantation. We cultured AT-MSC with standard medium and media used to culture LSC for clinical application. We demonstrated that different media changed the AT-MSC paracrine secretion showing different paracrine effector functions in an in vivo model of chemical burn and in response to a novel in vitro model of corneal inflammation by alkali induction. Treatment of LSCD with AT-MSC changed the angiogenic and inflammatory cytokine profile of mice corneas. AT-MSC cultured with the medium that improved their cytokine secretion, enhanced the anti-angiogenic and anti-inflammatory profile of the treated corneas. Those corneas also presented better outcome in terms of corneal transparency, neovascularization and histologic reconstruction. Priming human AT-MSC with LSC specific medium can potentiate their ability to improve corneal wound healing, decrease neovascularization and inflammation modulating paracrine effector functions in an in vivo optimized rat model of LSCD.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/269725
https://dx.doi.org/urn:doi:10.1111/jcmm.16501
url https://ddd.uab.cat/record/269725
https://dx.doi.org/urn:doi:10.1111/jcmm.16501
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FIS14-PI00196
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FIS18-PI00355
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI15-01447
Ministerio de Educación, Cultura y Deporte https://doi.org/10.13039/501100003176 AP2010-0659
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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