Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity

Understanding the genetic factors involved in seed longevity is of paramount importance in agricultural and ecological contexts. The polygenic nature of this trait suggests that many of them remain undiscovered. Here, we exploited the contrasting seed longevity found amongst Arabidopsis thaliana acc...

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Detalles Bibliográficos
Autores: Niñoles, Regina, Planes, Dolores, Arjona, Paloma, Ruiz-Pastor, Carmen, Chazarra, Rubén, Renard, Joan, Bueso, Eduardo, Forment, Javier, Serrano, Ramón, Kranner, Ilse, Roach, Thomas, Gadea, José
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304825
Acceso en línea:http://hdl.handle.net/10261/304825
https://api.elsevier.com/content/abstract/scopus_id/85132374308
Access Level:acceso abierto
Palabra clave:arabidopsis
glutathione
longevity
natural variation
seed
transcriptomics
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spelling Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevityNiñoles, ReginaPlanes, DoloresArjona, PalomaRuiz-Pastor, CarmenChazarra, RubénRenard, JoanBueso, EduardoForment, JavierSerrano, RamónKranner, IlseRoach, ThomasGadea, Joséarabidopsisglutathionelongevitynatural variationseedtranscriptomicsUnderstanding the genetic factors involved in seed longevity is of paramount importance in agricultural and ecological contexts. The polygenic nature of this trait suggests that many of them remain undiscovered. Here, we exploited the contrasting seed longevity found amongst Arabidopsis thaliana accessions to further understand this phenomenon. Concentrations of glutathione were higher in longer-lived than shorter-lived accessions, supporting that redox poise plays a prominent role in seed longevity. However, high seed permeability, normally associated with shorter longevity, is also present in long-lived accessions. Dry seed transcriptome analysis indicated that the contribution to longevity of stored messenger RNA (mRNAs) is complex, including mainly accession-specific mechanisms. The detrimental effect on longevity caused by other factors may be counterbalanced by higher levels of specific mRNAs stored in dry seeds, for instance those of heat-shock proteins. Indeed, loss-of-function mutant analysis demonstrated that heat-shock factors HSF1A and 1B contributed to longevity. Furthermore, mutants of the stress-granule zinc-finger protein TZF9 or the spliceosome subunits MOS4 or MAC3A/MAC3B, extended seed longevity, positioning RNA as a novel player in the regulation of seed viability. mRNAs of proteins with putative relevance to longevity were also abundant in shorter-lived accessions, reinforcing the idea that resistance to ageing is determined by multiple factors.Peer reviewed0000-0003-1797-15780000-0002-3612-7914Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/304825https://api.elsevier.com/content/abstract/scopus_id/85132374308reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésPlant, cell & environmentSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3048252026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
spellingShingle Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
Niñoles, Regina
arabidopsis
glutathione
longevity
natural variation
seed
transcriptomics
title_short Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_full Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_fullStr Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_full_unstemmed Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
title_sort Comparative analysis of wild-type accessions reveals novel determinants of Arabidopsis seed longevity
dc.creator.none.fl_str_mv Niñoles, Regina
Planes, Dolores
Arjona, Paloma
Ruiz-Pastor, Carmen
Chazarra, Rubén
Renard, Joan
Bueso, Eduardo
Forment, Javier
Serrano, Ramón
Kranner, Ilse
Roach, Thomas
Gadea, José
author Niñoles, Regina
author_facet Niñoles, Regina
Planes, Dolores
Arjona, Paloma
Ruiz-Pastor, Carmen
Chazarra, Rubén
Renard, Joan
Bueso, Eduardo
Forment, Javier
Serrano, Ramón
Kranner, Ilse
Roach, Thomas
Gadea, José
author_role author
author2 Planes, Dolores
Arjona, Paloma
Ruiz-Pastor, Carmen
Chazarra, Rubén
Renard, Joan
Bueso, Eduardo
Forment, Javier
Serrano, Ramón
Kranner, Ilse
Roach, Thomas
Gadea, José
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv 0000-0003-1797-1578
0000-0002-3612-7914
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv arabidopsis
glutathione
longevity
natural variation
seed
transcriptomics
topic arabidopsis
glutathione
longevity
natural variation
seed
transcriptomics
description Understanding the genetic factors involved in seed longevity is of paramount importance in agricultural and ecological contexts. The polygenic nature of this trait suggests that many of them remain undiscovered. Here, we exploited the contrasting seed longevity found amongst Arabidopsis thaliana accessions to further understand this phenomenon. Concentrations of glutathione were higher in longer-lived than shorter-lived accessions, supporting that redox poise plays a prominent role in seed longevity. However, high seed permeability, normally associated with shorter longevity, is also present in long-lived accessions. Dry seed transcriptome analysis indicated that the contribution to longevity of stored messenger RNA (mRNAs) is complex, including mainly accession-specific mechanisms. The detrimental effect on longevity caused by other factors may be counterbalanced by higher levels of specific mRNAs stored in dry seeds, for instance those of heat-shock proteins. Indeed, loss-of-function mutant analysis demonstrated that heat-shock factors HSF1A and 1B contributed to longevity. Furthermore, mutants of the stress-granule zinc-finger protein TZF9 or the spliceosome subunits MOS4 or MAC3A/MAC3B, extended seed longevity, positioning RNA as a novel player in the regulation of seed viability. mRNAs of proteins with putative relevance to longevity were also abundant in shorter-lived accessions, reinforcing the idea that resistance to ageing is determined by multiple factors.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/304825
https://api.elsevier.com/content/abstract/scopus_id/85132374308
url http://hdl.handle.net/10261/304825
https://api.elsevier.com/content/abstract/scopus_id/85132374308
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plant, cell & environment
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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