Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response

10 pags., 10 figs.

Detalhes bibliográficos
Autores: Lorenzo-Martín, Elvira, Gallego-Muñoz, Patricia, Ibares-Frías, L., Marcos, Susana, Pérez Merino, Pablo, Fernández, Itziar, Kochevar, Irene E., Martínez-García, María Carmen
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/249880
Acesso em linha:http://hdl.handle.net/10261/249880
Access Level:acceso abierto
Palavra-chave:Wound repair
Cross-linking
UVA radiation
Cornea
Rose bengal
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spelling Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair responseLorenzo-Martín, ElviraGallego-Muñoz, PatriciaIbares-Frías, L.Marcos, SusanaPérez Merino, PabloFernández, ItziarKochevar, Irene E.Martínez-García, María CarmenWound repairCross-linkingUVA radiationCorneaRose bengal10 pags., 10 figs.PURPOSE. To study corneal wound healing after two cross-linking techniques using either rose bengal and green light (RGX) or the conventional treatment using riboflavin and UVA radiation (UVX). METHODS. Corneas of New Zealand rabbits were monolaterally treated with UVX (21 eyes) or RGX (25 eyes). Treatments involved corneal de-epithelialization (8-mm diameter), soaking with photosensitizer (0.1% riboflavin in 20% dextran for 30 minutes for UVX; 0.1% rose bengal for 2 minutes for RGX), and light irradiation (370 nm, 3 mW/cm, 30 minutes for UVX; 532 nm, 0.25 W/cm, 7 minutes for RGX). Contralateral eyes were used as controls. Clinical follow-up included fluorescein staining, haze measurement, and pachymetry. Healing events analyzed after euthanasia at 2, 30, and 60 days included cell death (TUNEL assay), cell proliferation (BrdU [bromodeoxyuridine] immunofluorescence), and differentiation to myofibroblasts (α-SMA [alpha smooth muscle actin] immunohistochemistry). RESULTS. Re-epithelialization and pachymetries were similar after RGX and UVX. The haze from day 1 to 15 was greater after UVX. Cell death was deeper after UVX, being localized in the anterior and middle stroma, and was superficial (anterior third) after RGX. Cell proliferation appeared after 2 days and was localized in the middle and posterior stroma in the UVX group but was superficial in the RGX group. After 60 days the number of stromal cells had not returned to the control number in either group. CONCLUSIONS. The deeper and longer-lasting cell damage caused by UVX compared to RGX may underlie the slower cell repopulation after UVX and other differences in healing. Shallower damage and a shorter treatment time suggest that RGX may be appropriate for stiffening thin corneas.Supported by European Research Council ERC-2011-AdC-294099 (SM), Spanish Government Grants FIS2011-25637-R (SM), FIS2014- 56643-R, and FIS2017-84753-R (SM)Association for Research in Vision and OphthalmologyEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120182021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/249880reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/294099info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2011-25637-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2014-56643-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2017-84753http://dx.doi.org/10.1167/iovs.18-24881Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2498802026-05-22T06:33:51Z
dc.title.none.fl_str_mv Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
spellingShingle Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
Lorenzo-Martín, Elvira
Wound repair
Cross-linking
UVA radiation
Cornea
Rose bengal
title_short Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_full Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_fullStr Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_full_unstemmed Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
title_sort Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
dc.creator.none.fl_str_mv Lorenzo-Martín, Elvira
Gallego-Muñoz, Patricia
Ibares-Frías, L.
Marcos, Susana
Pérez Merino, Pablo
Fernández, Itziar
Kochevar, Irene E.
Martínez-García, María Carmen
author Lorenzo-Martín, Elvira
author_facet Lorenzo-Martín, Elvira
Gallego-Muñoz, Patricia
Ibares-Frías, L.
Marcos, Susana
Pérez Merino, Pablo
Fernández, Itziar
Kochevar, Irene E.
Martínez-García, María Carmen
author_role author
author2 Gallego-Muñoz, Patricia
Ibares-Frías, L.
Marcos, Susana
Pérez Merino, Pablo
Fernández, Itziar
Kochevar, Irene E.
Martínez-García, María Carmen
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Wound repair
Cross-linking
UVA radiation
Cornea
Rose bengal
topic Wound repair
Cross-linking
UVA radiation
Cornea
Rose bengal
description 10 pags., 10 figs.
publishDate 2018
dc.date.none.fl_str_mv 2018
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/249880
url http://hdl.handle.net/10261/249880
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/294099
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2011-25637-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2014-56643-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FIS2017-84753
http://dx.doi.org/10.1167/iovs.18-24881
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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