Acetylome in Human Fibroblasts From Parkinson's Disease Patients

Parkinson's disease (PD) is amultifactorial neurodegenerative disorder. The pathogenesis of this disease is associated with gene and environmental factors. Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent genetic cause of familial and sporadic PD. Moreover, posttranslatio...

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Detalles Bibliográficos
Autores: Yakhine Diop, Sokhna M. S., Rodríguez Arribas, Mario, Martínez Chacón, Guadalupe, Uribe Carretero, Elisabet, Gómez Sánchez, Rubén, Aiastui, Ana, López de Munain Arregui, Adolfo José, Bravo San Pedro, José M., Niso Santano, Mireia, González Polo, Rosa A., Fuentes Rodríguez, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/30349
Acceso en línea:http://hdl.handle.net/10810/30349
Access Level:acceso abierto
Palabra clave:acetylation
LRRK2
peptides
parkinson
proteins
serotonergic dysfunction
histone acetylation
tubulin acetylation
autophagy
acetyltransferase
hyperacetylation
ubiquitin
kinase
Descripción
Sumario:Parkinson's disease (PD) is amultifactorial neurodegenerative disorder. The pathogenesis of this disease is associated with gene and environmental factors. Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent genetic cause of familial and sporadic PD. Moreover, posttranslational modifications, including protein acetylation, are involved in the molecular mechanism of PD. Acetylation of lysine proteins is a dynamic process that ismodulated in PD. In this descriptive study, we characterized the acetylated proteins and peptides in primary fibroblasts from idiopathic PD (IPD) and genetic PD harboring G2019S or R1441G LRRK2 mutations. Identified acetylated peptides are modulated between individuals' groups. Although acetylated nuclear proteins are the most represented in cells, they are hypoacetylated in IPD. Results display that the level of hyperacetylated and hypoacetylated peptides are, respectively, enhanced in genetic PD and in IPD cells.