CONSTANS alters the circadian clock in Arabidopsis thaliana

Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic p...

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Autores: Reyes, Pedro de los, Serrano Bueno, Gloria, Romero Campero, Francisco José, Gao, He, Romero Rodríguez, José María, Valverde, Federico
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/164966
Acceso en línea:https://hdl.handle.net/11441/164966
https://doi.org/10.1016/j.molp.2024.06.006
Access Level:acceso abierto
Palabra clave:Circadian clock
CONSTANS
Photoperiod
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spelling CONSTANS alters the circadian clock in Arabidopsis thalianaReyes, Pedro de losSerrano Bueno, GloriaRomero Campero, Francisco JoséGao, HeRomero Rodríguez, José MaríaValverde, FedericoCircadian clockCONSTANSPhotoperiodPlants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic pathway, which controls plant response to day length, is one of the most important pathways controlling flowering. In Arabidopsis photoperiodic flowering, CONSTANS (CO) is the central gene activating the expression of the florigen FLOWERING LOCUS T (FT) in the leaves at the end of a long day. The circadian clock strongly regulates CO expression. However, to date, no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock. Using transcriptional networks, we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway. Gene expression, chromatin immunoprecipitation experiments, and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock. Plants with altered CO expression showed a different internal clock period, measured by daily leaf rhythmic movements. We showed that CO upregulates the expression of key genes related to the circadian clock, such as CCA1, LHY, PRR5, and GI, at the end of a long day by binding to specific sites on their promoters. Moreover, a high number of PRR5-repressed target genes are upregulated by CO, and this could explain the phase transition promoted by CO. The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes. Taken together, our results indicate that there may be a feedback loop in which CO communicates back to the circadian clock, providing seasonal information to the circadian system.Ministerio de Ciencia, Innovación y Universidades (MICINN) PID2020-117018RB-I00ElsevierBioquímica Vegetal y Biología MolecularCiencias de la Computación e Inteligencia ArtificialMinisterio de Ciencia, Innovación y Universidades (MICINN). España2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/164966https://doi.org/10.1016/j.molp.2024.06.006reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Plant, 17 (8), 1204-1220.PID2020-117018RB-I00https://doi.org/10.1016/j.molp.2024.06.006info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1649662026-06-17T12:51:07Z
dc.title.none.fl_str_mv CONSTANS alters the circadian clock in Arabidopsis thaliana
title CONSTANS alters the circadian clock in Arabidopsis thaliana
spellingShingle CONSTANS alters the circadian clock in Arabidopsis thaliana
Reyes, Pedro de los
Circadian clock
CONSTANS
Photoperiod
title_short CONSTANS alters the circadian clock in Arabidopsis thaliana
title_full CONSTANS alters the circadian clock in Arabidopsis thaliana
title_fullStr CONSTANS alters the circadian clock in Arabidopsis thaliana
title_full_unstemmed CONSTANS alters the circadian clock in Arabidopsis thaliana
title_sort CONSTANS alters the circadian clock in Arabidopsis thaliana
dc.creator.none.fl_str_mv Reyes, Pedro de los
Serrano Bueno, Gloria
Romero Campero, Francisco José
Gao, He
Romero Rodríguez, José María
Valverde, Federico
author Reyes, Pedro de los
author_facet Reyes, Pedro de los
Serrano Bueno, Gloria
Romero Campero, Francisco José
Gao, He
Romero Rodríguez, José María
Valverde, Federico
author_role author
author2 Serrano Bueno, Gloria
Romero Campero, Francisco José
Gao, He
Romero Rodríguez, José María
Valverde, Federico
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Ciencias de la Computación e Inteligencia Artificial
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
dc.subject.none.fl_str_mv Circadian clock
CONSTANS
Photoperiod
topic Circadian clock
CONSTANS
Photoperiod
description Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic pathway, which controls plant response to day length, is one of the most important pathways controlling flowering. In Arabidopsis photoperiodic flowering, CONSTANS (CO) is the central gene activating the expression of the florigen FLOWERING LOCUS T (FT) in the leaves at the end of a long day. The circadian clock strongly regulates CO expression. However, to date, no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock. Using transcriptional networks, we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway. Gene expression, chromatin immunoprecipitation experiments, and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock. Plants with altered CO expression showed a different internal clock period, measured by daily leaf rhythmic movements. We showed that CO upregulates the expression of key genes related to the circadian clock, such as CCA1, LHY, PRR5, and GI, at the end of a long day by binding to specific sites on their promoters. Moreover, a high number of PRR5-repressed target genes are upregulated by CO, and this could explain the phase transition promoted by CO. The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes. Taken together, our results indicate that there may be a feedback loop in which CO communicates back to the circadian clock, providing seasonal information to the circadian system.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/164966
https://doi.org/10.1016/j.molp.2024.06.006
url https://hdl.handle.net/11441/164966
https://doi.org/10.1016/j.molp.2024.06.006
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Plant, 17 (8), 1204-1220.
PID2020-117018RB-I00
https://doi.org/10.1016/j.molp.2024.06.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
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collection idUS. Depósito de Investigación de la Universidad de Sevilla
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