Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity

The use of lactic acid bacteria (LAB) as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. The objectives of this work were (1) the in vitro safety assessment of 8 Pediococcus acidilactici strains isolated from rainbow...

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Autores: Dias Araújo, Carlos Alexandre, Muñoz Atienza, Estefanía, Poeta, P., Igrejas, G., Hernández Cruza, Pablo Elpidio, Herranz Sorribes, Carmen, Cintas Izarra, Luis Miguel
Formato: artículo
Fecha de publicación:2016
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/95012
Acesso em linha:https://hdl.handle.net/20.500.14352/95012
Access Level:acceso abierto
Palavra-chave:61
Aquaculture
Oncorhynchus mykiss
Lactic acid bacteria
Anti-fish pathogen activity
Fish probiotics
ERIC-PCR
Ciencias Biomédicas
32 Ciencias Médicas
24 Ciencias de la Vida
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network_acronym_str ES
network_name_str España
repository_id_str
spelling Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicityDias Araújo, Carlos AlexandreMuñoz Atienza, EstefaníaPoeta, P.Igrejas, G.Hernández Cruza, Pablo ElpidioHerranz Sorribes, CarmenCintas Izarra, Luis Miguel61AquacultureOncorhynchus mykissLactic acid bacteriaAnti-fish pathogen activityFish probioticsERIC-PCRCiencias Biomédicas32 Ciencias Médicas24 Ciencias de la VidaThe use of lactic acid bacteria (LAB) as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. The objectives of this work were (1) the in vitro safety assessment of 8 Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae; (2) the evaluation of their genetic relatedness; (3) the study of their antimicrobial/bacteriocin activity against fish pathogens; and (4) the biochemical and genetic characterization of the bacteriocin produced by the strain displaying the greatest antimicrobial activity. Concerning the safety assessment, none of the pediococci showed antibiotic resistance nor produced hemolysin or gelatinase, degraded gastric mucin, or deconjugated bile salts. Four strains (50%) produced tyramine or putrescine, but the corresponding genes were not amplified by PCR. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting allowed clustering of the pediococci into 2 well-defined groups (68% similarity). From the 8 pediococci displaying direct antimicrobial activity against at least 3 out of 9 fish pathogens, 6 strains (75%) were identified as bacteriocin producers. The bacteriocin produced by P. acidilactici L-14 was purified, and mass spectrometry and DNA sequencing revealed its identity to pediocin PA-1 (PedPA-1). Altogether, our results allowed the identification of 4 (50%) putatively safe pediococci, including 2 bacteriocinogenic strains. ERIC-PCR fingerprinting was a valuable tool for genetic profiling of P. acidilactici strains. This work reports for the first time the characterization of a PedPA-1-producing P. acidilactici strain isolated from an aquatic environment (rainbow trout larvae), which shows interesting properties related to its potential use as a probiotic in aquaculture.Universidad Complutense de Madrid20162016-05-0320162016-05-03journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/95012reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/950122026-06-02T12:44:21Z
dc.title.none.fl_str_mv Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
title Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
spellingShingle Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
Dias Araújo, Carlos Alexandre
61
Aquaculture
Oncorhynchus mykiss
Lactic acid bacteria
Anti-fish pathogen activity
Fish probiotics
ERIC-PCR
Ciencias Biomédicas
32 Ciencias Médicas
24 Ciencias de la Vida
title_short Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
title_full Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
title_fullStr Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
title_full_unstemmed Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
title_sort Characterization of Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae: safety, DNA fingerprinting and bacteriocinogenicity
dc.creator.none.fl_str_mv Dias Araújo, Carlos Alexandre
Muñoz Atienza, Estefanía
Poeta, P.
Igrejas, G.
Hernández Cruza, Pablo Elpidio
Herranz Sorribes, Carmen
Cintas Izarra, Luis Miguel
author Dias Araújo, Carlos Alexandre
author_facet Dias Araújo, Carlos Alexandre
Muñoz Atienza, Estefanía
Poeta, P.
Igrejas, G.
Hernández Cruza, Pablo Elpidio
Herranz Sorribes, Carmen
Cintas Izarra, Luis Miguel
author_role author
author2 Muñoz Atienza, Estefanía
Poeta, P.
Igrejas, G.
Hernández Cruza, Pablo Elpidio
Herranz Sorribes, Carmen
Cintas Izarra, Luis Miguel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 61
Aquaculture
Oncorhynchus mykiss
Lactic acid bacteria
Anti-fish pathogen activity
Fish probiotics
ERIC-PCR
Ciencias Biomédicas
32 Ciencias Médicas
24 Ciencias de la Vida
topic 61
Aquaculture
Oncorhynchus mykiss
Lactic acid bacteria
Anti-fish pathogen activity
Fish probiotics
ERIC-PCR
Ciencias Biomédicas
32 Ciencias Médicas
24 Ciencias de la Vida
description The use of lactic acid bacteria (LAB) as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. The objectives of this work were (1) the in vitro safety assessment of 8 Pediococcus acidilactici strains isolated from rainbow trout (Oncorhynchus mykiss, Walbaum) feed and larvae; (2) the evaluation of their genetic relatedness; (3) the study of their antimicrobial/bacteriocin activity against fish pathogens; and (4) the biochemical and genetic characterization of the bacteriocin produced by the strain displaying the greatest antimicrobial activity. Concerning the safety assessment, none of the pediococci showed antibiotic resistance nor produced hemolysin or gelatinase, degraded gastric mucin, or deconjugated bile salts. Four strains (50%) produced tyramine or putrescine, but the corresponding genes were not amplified by PCR. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting allowed clustering of the pediococci into 2 well-defined groups (68% similarity). From the 8 pediococci displaying direct antimicrobial activity against at least 3 out of 9 fish pathogens, 6 strains (75%) were identified as bacteriocin producers. The bacteriocin produced by P. acidilactici L-14 was purified, and mass spectrometry and DNA sequencing revealed its identity to pediocin PA-1 (PedPA-1). Altogether, our results allowed the identification of 4 (50%) putatively safe pediococci, including 2 bacteriocinogenic strains. ERIC-PCR fingerprinting was a valuable tool for genetic profiling of P. acidilactici strains. This work reports for the first time the characterization of a PedPA-1-producing P. acidilactici strain isolated from an aquatic environment (rainbow trout larvae), which shows interesting properties related to its potential use as a probiotic in aquaculture.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-05-03
2016
2016-05-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/95012
url https://hdl.handle.net/20.500.14352/95012
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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