Why not glycine electrochemical biosensors?

Glycine monitoring is gaining importance as a biomarker in clinical analysis due to its involvement in multiple physiological functions, which results in glycine being one of the most analyzed biomolecules for diagnostics. This growing demand requires faster and more reliable, while affordable, anal...

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Autores: Pérez Ràfols, Clara, Liu, Yujie, Wang, Qianyu, Cuartero, María, Crespo, Gastón A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/173834
Acceso en línea:https://hdl.handle.net/2445/173834
Access Level:acceso abierto
Palabra clave:Electroquímica
Biosensors
Assistència sanitària
Electrochemistry
Medical care
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spelling Why not glycine electrochemical biosensors?Pérez Ràfols, ClaraLiu, YujieWang, QianyuCuartero, MaríaCrespo, Gastón A.ElectroquímicaBiosensorsAssistència sanitàriaElectrochemistryBiosensorsMedical careGlycine monitoring is gaining importance as a biomarker in clinical analysis due to its involvement in multiple physiological functions, which results in glycine being one of the most analyzed biomolecules for diagnostics. This growing demand requires faster and more reliable, while affordable, analytical methods that can replace the current gold standard for glycine detection, which is based on sample extraction with subsequent use of liquid chromatography or fluorometric kits for its quantification in centralized laboratories. This work discusses electrochemical sensors and biosensors as an alternative option, focusing on their potential application for glycine determination in blood, urine, and cerebrospinal fluid, the three most widely used matrices for glycine analysis with clinical meaning. For electrochemical sensors, voltammetry/amperometry is the preferred readout (10 of the 13 papers collected in this review) and metal-based redox mediator modification is the predominant approach for electrode fabrication (11 of the 13 papers). However, none of the reported electrochemical sensors fulfill the requirements for direct analysis of biological fluids, most of them lacking appropriate selectivity, linear range of response, and/or capability of measuring at physiological conditions. Enhanced selectivity has been recently reported using biosensors (with an enzyme element in the electrode design), although this is still a very incipient approach. Currently, despite the benefits of electrochemistry, only optical biosensors have been successfully reported for glycine detection and, from all the inspected works, it is clear that bioengineering efforts will play a key role in the embellishment of selectivity and storage stability of the sensing element in the sensor.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/173834Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/s20144049Sensors, 2020, vol. 20, num. 14, p. 4049https://doi.org/10.3390/s20144049cc-by (c) Pérez Ràfols, Clara et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1738342026-05-27T06:46:51Z
dc.title.none.fl_str_mv Why not glycine electrochemical biosensors?
title Why not glycine electrochemical biosensors?
spellingShingle Why not glycine electrochemical biosensors?
Pérez Ràfols, Clara
Electroquímica
Biosensors
Assistència sanitària
Electrochemistry
Biosensors
Medical care
title_short Why not glycine electrochemical biosensors?
title_full Why not glycine electrochemical biosensors?
title_fullStr Why not glycine electrochemical biosensors?
title_full_unstemmed Why not glycine electrochemical biosensors?
title_sort Why not glycine electrochemical biosensors?
dc.creator.none.fl_str_mv Pérez Ràfols, Clara
Liu, Yujie
Wang, Qianyu
Cuartero, María
Crespo, Gastón A.
author Pérez Ràfols, Clara
author_facet Pérez Ràfols, Clara
Liu, Yujie
Wang, Qianyu
Cuartero, María
Crespo, Gastón A.
author_role author
author2 Liu, Yujie
Wang, Qianyu
Cuartero, María
Crespo, Gastón A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Electroquímica
Biosensors
Assistència sanitària
Electrochemistry
Biosensors
Medical care
topic Electroquímica
Biosensors
Assistència sanitària
Electrochemistry
Biosensors
Medical care
description Glycine monitoring is gaining importance as a biomarker in clinical analysis due to its involvement in multiple physiological functions, which results in glycine being one of the most analyzed biomolecules for diagnostics. This growing demand requires faster and more reliable, while affordable, analytical methods that can replace the current gold standard for glycine detection, which is based on sample extraction with subsequent use of liquid chromatography or fluorometric kits for its quantification in centralized laboratories. This work discusses electrochemical sensors and biosensors as an alternative option, focusing on their potential application for glycine determination in blood, urine, and cerebrospinal fluid, the three most widely used matrices for glycine analysis with clinical meaning. For electrochemical sensors, voltammetry/amperometry is the preferred readout (10 of the 13 papers collected in this review) and metal-based redox mediator modification is the predominant approach for electrode fabrication (11 of the 13 papers). However, none of the reported electrochemical sensors fulfill the requirements for direct analysis of biological fluids, most of them lacking appropriate selectivity, linear range of response, and/or capability of measuring at physiological conditions. Enhanced selectivity has been recently reported using biosensors (with an enzyme element in the electrode design), although this is still a very incipient approach. Currently, despite the benefits of electrochemistry, only optical biosensors have been successfully reported for glycine detection and, from all the inspected works, it is clear that bioengineering efforts will play a key role in the embellishment of selectivity and storage stability of the sensing element in the sensor.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/173834
url https://hdl.handle.net/2445/173834
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/s20144049
Sensors, 2020, vol. 20, num. 14, p. 4049
https://doi.org/10.3390/s20144049
dc.rights.none.fl_str_mv cc-by (c) Pérez Ràfols, Clara et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Pérez Ràfols, Clara et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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