A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging

In vitro analysis requires cell proliferation in conditions close to physiological ones. Lab-on-a-chip (LoC) devices simplify, miniaturize and automate traditional protocols, with the advantages of being less expensive and faster due to their shorter diffusion distances. The main limitation of curre...

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Detalles Bibliográficos
Autores: Giménez-Gómez, Pablo, Rodríguez-Rodríguez, Rosalía, Ríos, Juan Manuel, Pérez-Montero, Marta, González, Estrella, Gutiérrez-Capitán, Manuel, Plaza, José Antonio, Muñoz-Berbel, Xavier, Jiménez-Jorquera, Cecilia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376932
Acceso en línea:http://hdl.handle.net/10261/376932
https://api.elsevier.com/content/abstract/scopus_id/85079775439
Access Level:acceso abierto
Palabra clave:Biofouling
Cell proliferation
Cost effectiveness
Electrochemical biosensors
Electrochemical electrodes
Lab-on-a-chip
Redox reactions
Risk assessment
Transducers
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spelling A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imagingGiménez-Gómez, PabloRodríguez-Rodríguez, RosalíaRíos, Juan ManuelPérez-Montero, MartaGonzález, EstrellaGutiérrez-Capitán, ManuelPlaza, José AntonioMuñoz-Berbel, XavierJiménez-Jorquera, CeciliaBiofoulingCell proliferationCost effectivenessElectrochemical biosensorsElectrochemical electrodesLab-on-a-chipRedox reactionsRisk assessmentTransducersIn vitro analysis requires cell proliferation in conditions close to physiological ones. Lab-on-a-chip (LoC) devices simplify, miniaturize and automate traditional protocols, with the advantages of being less expensive and faster due to their shorter diffusion distances. The main limitation of current LoCs is still the control of the culture conditions. Most LoCs employ off-chip equipment to determine cell culture activity, which confers limited monitoring capacity. The few systems integrating transducers on-chip present important functional problems mostly associated with the attachment of biomolecules to the transducer surface (i.e., biofouling) and the impossibility of re-calibrating the sensors during cell culturing. This limitation is addressed in the present LoC containing a network of micro-channels and micro-chambers, which allows (i) cell seeding and cultivation, avoiding biofouling risk, (ii) multiplexed analysis of cell culture, reactivation and recalibration of the (bio)sensors without compromising cell viability, (iii) cell imaging and (iv) reference electrode compartmentalization to guarantee stability. The activity of the culture is monitored with four independent electrochemical micro-electrodes for glucose, hydrogen peroxide, conductivity and oxidation reduction potential. Electrochemical analysis is complemented with high-resolution confocal microscopy analysis. This paper demonstrates the suitability of the current configuration for cell culture monitoring and future applications in drug screening or organ-on-a-chip development.This work was supported by the Spanish R & D National Program (MEC Project TEC2014-54449-C3-1-R and RTI2018-100773-B-C31).Peer reviewedRoyal Society of Chemistry (UK)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/376932https://api.elsevier.com/content/abstract/scopus_id/85079775439reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100773-B-C31info:eu-repo/grantAgreement/MINECO//TEC2014-54449-C3-1-RLab on a chiphttps://pubs.rsc.org/en/content/articlepdf/2020/lc/c9lc01051cSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3769322026-05-22T06:33:51Z
dc.title.none.fl_str_mv A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
title A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
spellingShingle A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
Giménez-Gómez, Pablo
Biofouling
Cell proliferation
Cost effectiveness
Electrochemical biosensors
Electrochemical electrodes
Lab-on-a-chip
Redox reactions
Risk assessment
Transducers
title_short A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
title_full A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
title_fullStr A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
title_full_unstemmed A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
title_sort A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
dc.creator.none.fl_str_mv Giménez-Gómez, Pablo
Rodríguez-Rodríguez, Rosalía
Ríos, Juan Manuel
Pérez-Montero, Marta
González, Estrella
Gutiérrez-Capitán, Manuel
Plaza, José Antonio
Muñoz-Berbel, Xavier
Jiménez-Jorquera, Cecilia
author Giménez-Gómez, Pablo
author_facet Giménez-Gómez, Pablo
Rodríguez-Rodríguez, Rosalía
Ríos, Juan Manuel
Pérez-Montero, Marta
González, Estrella
Gutiérrez-Capitán, Manuel
Plaza, José Antonio
Muñoz-Berbel, Xavier
Jiménez-Jorquera, Cecilia
author_role author
author2 Rodríguez-Rodríguez, Rosalía
Ríos, Juan Manuel
Pérez-Montero, Marta
González, Estrella
Gutiérrez-Capitán, Manuel
Plaza, José Antonio
Muñoz-Berbel, Xavier
Jiménez-Jorquera, Cecilia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biofouling
Cell proliferation
Cost effectiveness
Electrochemical biosensors
Electrochemical electrodes
Lab-on-a-chip
Redox reactions
Risk assessment
Transducers
topic Biofouling
Cell proliferation
Cost effectiveness
Electrochemical biosensors
Electrochemical electrodes
Lab-on-a-chip
Redox reactions
Risk assessment
Transducers
description In vitro analysis requires cell proliferation in conditions close to physiological ones. Lab-on-a-chip (LoC) devices simplify, miniaturize and automate traditional protocols, with the advantages of being less expensive and faster due to their shorter diffusion distances. The main limitation of current LoCs is still the control of the culture conditions. Most LoCs employ off-chip equipment to determine cell culture activity, which confers limited monitoring capacity. The few systems integrating transducers on-chip present important functional problems mostly associated with the attachment of biomolecules to the transducer surface (i.e., biofouling) and the impossibility of re-calibrating the sensors during cell culturing. This limitation is addressed in the present LoC containing a network of micro-channels and micro-chambers, which allows (i) cell seeding and cultivation, avoiding biofouling risk, (ii) multiplexed analysis of cell culture, reactivation and recalibration of the (bio)sensors without compromising cell viability, (iii) cell imaging and (iv) reference electrode compartmentalization to guarantee stability. The activity of the culture is monitored with four independent electrochemical micro-electrodes for glucose, hydrogen peroxide, conductivity and oxidation reduction potential. Electrochemical analysis is complemented with high-resolution confocal microscopy analysis. This paper demonstrates the suitability of the current configuration for cell culture monitoring and future applications in drug screening or organ-on-a-chip development.
publishDate 2020
dc.date.none.fl_str_mv 2020
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376932
https://api.elsevier.com/content/abstract/scopus_id/85079775439
url http://hdl.handle.net/10261/376932
https://api.elsevier.com/content/abstract/scopus_id/85079775439
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100773-B-C31
info:eu-repo/grantAgreement/MINECO//TEC2014-54449-C3-1-R
Lab on a chip
https://pubs.rsc.org/en/content/articlepdf/2020/lc/c9lc01051c
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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