High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation

Additive manufacturing technology is an emerging method for rapid prototyping, which enables the creation of complex geometries by one-step fabrication processes through a layer-by-layer approach. The simplified fabrication achieved with this methodology opens the way towards a more efficient indust...

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Autores: Garcia-Rey, Sandra, Nielsen, Jacob B., Nordin, Gregory, Woolley, Adam, Basabe Desmonts, Lourdes, Benito López, Fernando
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/57249
Acceso en línea:http://hdl.handle.net/10810/57249
Access Level:acceso abierto
Palabra clave:3D printing
stereolithography
high resolution
fabrication
whole blood
plasma separation
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spelling High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma SeparationGarcia-Rey, SandraNielsen, Jacob B.Nordin, GregoryWoolley, AdamBasabe Desmonts, LourdesBenito López, Fernando3D printingstereolithographyhigh resolutionfabricationwhole bloodplasma separationAdditive manufacturing technology is an emerging method for rapid prototyping, which enables the creation of complex geometries by one-step fabrication processes through a layer-by-layer approach. The simplified fabrication achieved with this methodology opens the way towards a more efficient industrial production, with applications in a great number of fields such as biomedical devices. In biomedicine, blood is the gold-standard biofluid for clinical analysis. However, blood cells generate analytical interferences in many test procedures; hence, it is important to separate plasma from blood cells before analytical testing of blood samples. In this research, a custom-made resin formulation combined with a high-resolution 3D printing methodology were used to achieve a methodology for the fast prototype optimization of an operative plasma separation modular device. Through an iterative process, 17 different prototypes were designed and fabricated with printing times ranging from 5 to 12 min. The final device was evaluated through colorimetric analysis, validating this fabrication approach for the qualitative assessment of plasma separation from whole blood. The 3D printing method used here demonstrates the great contribution that this microfluidic technology will bring to the plasma separation biomedical devices market.This research was funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 778001 (DNASurf), “Ministerio de Ciencia y Educación de España” under grant PID2020-120313GB-I00/AIE/10.13039/501100011033, the Basque Government (Grant IT1271-19) and the US National Institutes of Health (R01 EB027096 and R15 GM123405-02).MDPIEuropean Commission2022202220222022info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/57249reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/EC/H2020/778001info:eu-repo/grantAgreement/MICINN/PID2020-120313GB-I00/https://www.mdpi.com/2073-4360/14/13/2537info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/© 2022 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).oai:addi.ehu.eus:10810/572492026-06-18T09:23:17Z
dc.title.none.fl_str_mv High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
title High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
spellingShingle High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
Garcia-Rey, Sandra
3D printing
stereolithography
high resolution
fabrication
whole blood
plasma separation
title_short High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
title_full High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
title_fullStr High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
title_full_unstemmed High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
title_sort High-Resolution 3D Printing Fabrication of a Microfluidic Platform for Blood Plasma Separation
dc.creator.none.fl_str_mv Garcia-Rey, Sandra
Nielsen, Jacob B.
Nordin, Gregory
Woolley, Adam
Basabe Desmonts, Lourdes
Benito López, Fernando
author Garcia-Rey, Sandra
author_facet Garcia-Rey, Sandra
Nielsen, Jacob B.
Nordin, Gregory
Woolley, Adam
Basabe Desmonts, Lourdes
Benito López, Fernando
author_role author
author2 Nielsen, Jacob B.
Nordin, Gregory
Woolley, Adam
Basabe Desmonts, Lourdes
Benito López, Fernando
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
dc.subject.none.fl_str_mv 3D printing
stereolithography
high resolution
fabrication
whole blood
plasma separation
topic 3D printing
stereolithography
high resolution
fabrication
whole blood
plasma separation
description Additive manufacturing technology is an emerging method for rapid prototyping, which enables the creation of complex geometries by one-step fabrication processes through a layer-by-layer approach. The simplified fabrication achieved with this methodology opens the way towards a more efficient industrial production, with applications in a great number of fields such as biomedical devices. In biomedicine, blood is the gold-standard biofluid for clinical analysis. However, blood cells generate analytical interferences in many test procedures; hence, it is important to separate plasma from blood cells before analytical testing of blood samples. In this research, a custom-made resin formulation combined with a high-resolution 3D printing methodology were used to achieve a methodology for the fast prototype optimization of an operative plasma separation modular device. Through an iterative process, 17 different prototypes were designed and fabricated with printing times ranging from 5 to 12 min. The final device was evaluated through colorimetric analysis, validating this fabrication approach for the qualitative assessment of plasma separation from whole blood. The 3D printing method used here demonstrates the great contribution that this microfluidic technology will bring to the plasma separation biomedical devices market.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/57249
url http://hdl.handle.net/10810/57249
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/EC/H2020/778001
info:eu-repo/grantAgreement/MICINN/PID2020-120313GB-I00/
https://www.mdpi.com/2073-4360/14/13/2537
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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