Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification

The application of oxygen-dependent enzymes is limited by the low oxygen solubility, a fact that hinders the full operational exploitation of the enzyme activity. This oxygen limitation also creates a difficulty for understanding the intrinsic enzyme kinetics, a critical aspect for the process imple...

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Detalles Bibliográficos
Autores: Lorente Arevalo, Álvaro, Ladero Galán, Miguel, Bolívar Bolívar, Juan Manuel
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/116612
Acceso en línea:https://hdl.handle.net/20.500.14352/116612
Access Level:acceso abierto
Palabra clave:66.0
Ingeniería química
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
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spelling Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensificationLorente Arevalo, ÁlvaroLadero Galán, MiguelBolívar Bolívar, Juan Manuel66.0Ingeniería química3302 Tecnología Bioquímica3303 Ingeniería y Tecnología QuímicasThe application of oxygen-dependent enzymes is limited by the low oxygen solubility, a fact that hinders the full operational exploitation of the enzyme activity. This oxygen limitation also creates a difficulty for understanding the intrinsic enzyme kinetics, a critical aspect for the process implementation of oxidative enzymes. Kinetic analysis of O2-dependent enzymes is a case of ping-pong bi-substrate reaction kinetics but with the added feature of a fixed low concentration of oxygen dissolved in the liquid medium. We propose an analysis framework based on a combination of differential methods (based on initial reaction rates-concentration plots) to analyze the main substrate dependency, while the subsequent integral method (consumption time courses of oxygen dissolved) serves to analyze the oxygen dependency. The methodology is applicable by using the oxygen initially dissolved and only working with liquid suspensions. The analysis was applied to paradigmatic case studies with importance in modern green biooxidations. The modeling framework was validated and applied in scale-up reactions in an instrumented aerated stirred tank reactor.Universidad Complutense de Madrid20212021-08-2120212021-08-21journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/116612reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1166122026-06-02T12:44:21Z
dc.title.none.fl_str_mv Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
title Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
spellingShingle Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
Lorente Arevalo, Álvaro
66.0
Ingeniería química
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
title_short Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
title_full Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
title_fullStr Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
title_full_unstemmed Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
title_sort Suplementary information: framework of the kinetic analysis of O2 dependent oxidative biocatalysts for reaction intensification
dc.creator.none.fl_str_mv Lorente Arevalo, Álvaro
Ladero Galán, Miguel
Bolívar Bolívar, Juan Manuel
author Lorente Arevalo, Álvaro
author_facet Lorente Arevalo, Álvaro
Ladero Galán, Miguel
Bolívar Bolívar, Juan Manuel
author_role author
author2 Ladero Galán, Miguel
Bolívar Bolívar, Juan Manuel
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 66.0
Ingeniería química
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
topic 66.0
Ingeniería química
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
description The application of oxygen-dependent enzymes is limited by the low oxygen solubility, a fact that hinders the full operational exploitation of the enzyme activity. This oxygen limitation also creates a difficulty for understanding the intrinsic enzyme kinetics, a critical aspect for the process implementation of oxidative enzymes. Kinetic analysis of O2-dependent enzymes is a case of ping-pong bi-substrate reaction kinetics but with the added feature of a fixed low concentration of oxygen dissolved in the liquid medium. We propose an analysis framework based on a combination of differential methods (based on initial reaction rates-concentration plots) to analyze the main substrate dependency, while the subsequent integral method (consumption time courses of oxygen dissolved) serves to analyze the oxygen dependency. The methodology is applicable by using the oxygen initially dissolved and only working with liquid suspensions. The analysis was applied to paradigmatic case studies with importance in modern green biooxidations. The modeling framework was validated and applied in scale-up reactions in an instrumented aerated stirred tank reactor.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-08-21
2021
2021-08-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/116612
url https://hdl.handle.net/20.500.14352/116612
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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